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Straightforward thiol-mediated protein labelling with DTPA: Synthesis of a highly active 111In-annexin A5-DTPA tracer

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Figure

Figure 1 Binding affinity of anxA5-DTPA (6). Competitionexperiments demonstrate the inhibition of anxA5-FITC binding toapoptotic Jurkat T cells by active anxA5-DTPA, wildtype anxA5, andlack of inhibition by inactive M1234-anxA5-DTPA
Figure 2 Synthesis of anxA5-DTPA 6. 1) Excess of cyclic DTPA anhydride 1 was reacte d with N-(2-aminoethyl) maleimide trifluoroacetate 2 inhalf-saturated sodium bicabonate solution
Figure 4 Model of In-anxA5-DTPA. The blue spheres are Ca2+ inthe binding pockets at the PS-binding side of anxA5

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