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Evolutionary loss of melanogenesis in the tunicate Molgula occulta

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Figure

Fig. 1 Tyrosinase family evolution in tunicates. Maximum likelihood phylogeny of Tyrosinase members available in databases: The numbers at the branches represent the replicates come out employing ML method
Fig. 2 Expression of Tyrosinase genes during tunicate embryogenesis. The whole-mount in situ hybridization experiments show the localization of Tyrosinase gene expression in pigment cell precursors of three tunicates: Mooccu.Tyrp.a in Molgula occulta hatch
Fig. 3 GFP reporter assay of Molgula oculata Tyr promoter. A plasmid containing GFP has been used to demonstrate the conservation of Moocul.Tyrregulatory activity (1.3 Kb fragment) by electroporation of tunicate embryos
Fig. 4 Tyrosinase family pseudogenization in Molgula occulta. The figure points out the presence of premature stop codons (red) and frameshift insertions (magenta) in genomic sequences of Tyrp.a (a) and Tyr (b)
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