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Human papillomavirus type 16 E6 and E7 oncoproteins interact with the nuclear p53 binding protein 1 in an in vitro reconstructed 3D epithelium: new insights for the virus induced DNA damage response

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Academic year: 2020

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Figure

Fig. 1 H&E staining.III. Bar scales in 20x fields = 100keratinocytes. A) Control in vitro reconstructed 3D epithelial culture made up with normal keratinocytes and B) E6E7 HPV16 transduced C) In vivo normal epithelia obtained from tumor-free mucosa margins
Fig. 2 In vitro 3D epithelial cultures. HPV16 DNA (FISH) staining and immunofluorescent detection of BrdU, E6 and E7 of control normal and E6E7HPV16 infected reconstructed in vitro 3D epithelial cultures
Fig. 3 DDR characterization of the 3D in vitro reconstructed E6E7 HPV16 infected epithelia
Fig. 4 Double immunofluorescent analysis of E6 and E7 with cyclin E2 and B1. E6 and E7 HPV16 pushes a subset of differentiating cells into G1/Sand differentiated layers
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