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Identification of an intestine-specific promoter and inducible expression of bacterial α-galactosidase in mammalian cells by a lac operon system

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Figure

Table 1 Primers and regulatory element sequences used in this paper
Figure 1 Schematic structure of the intestine-specific promoters and operator. (A) Promoter/enhancer fusion constructs
Figure 2A shows that the HMUC2 promoter has thehighest activity among the intestine-specific promoterstested, which was about 2 to 3 times higher than that ofthe other three promoters (P < 0.05)
Figure 3 Effects of IPTG and α-lactose on the transient expression of luciferase and green fluorescent protein (GFP) activity aftertransfection of SW480 cells with luciferase-containing and GFP-containing plasmids, respectively
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