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Intracellular delivery of cell-penetrating peptide-transcriptional factor fusion protein and its role in selective osteogenesis

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Academic year: 2020

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Figure

Figure 1 (A–C) Identification of LMWP-TAZ fusion protein. Notes: (A) Purification of LMWP-TAZ employing Ni(II)-affinity chromatography was detected by Coomassie staining
Figure 2 (A–E) Quantification of LMWP-TAZ fusion protein transduction in hMSCs. Notes: (A) Flow-cytometric analysis of hMSCs treated with TAZ and LWMP-TAZ fusion proteins at a concentration of 100 nm for 20 minutes
Figure 3 (were performed in duplicate, and significant differences are denoted by asterisks (Abbreviations:A–C) Effect of osteogenic differentiation by LMWP-TAZ fusion protein
Figure 4 (A–C) Effect of adipogenic differentiation by LMWP-TAZ fusion protein. Notes: Cells were grown in adipogenic-inducing (AI) media and adipogenic-maintaining (AM) media with fusion proteins, as described in the Materials and methods section
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