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Tissue patterning in the developing mouse limb

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Figure

Fig. 1, A scanningelectronas fingers are now clearlyseparated Scale bars represent:becomesignsmicrographseries of mouseembryostakenat half-day intervalsduringthe period when the fore- and hindlimbsare developing.EachmicrographIS orientedso thattheforelimbi
Fig. 3. AralditehistologVregions.dndepidermisslf1gle cell diametercroached right up to the ridge epidermis.£13.5forelimb revealing digitsthrougha seriesof limbbuds.!A) Transversesectionthroughan E TOembryoar rhelevelofthe forelimb.At this stageno apical ec
Fig. 4. IA-CI Transversetheviewedrefringentcryo-sectionsthrougha seriesof embryos.cut atlevelof theforelimb,withmuscle/myogeniccellsrevealedby MF20immunohistochemistry;(D and EJ E13.5 and E14.5forelimbsrespectively,as wholemountsthroughcrossedpolaroidfilterstorevealbi.musclefibers.(AI El0.5The markedcells appearto have left thedermamyotome(0) adjacentto rhe neural rube (NT) and to be headmgtowardsthe base of the forelimb(arrow},Ventrallycardiacmusculaturehas sta,nedupdarkly.IS) EI J 5 The flTst sign of dorsal and ventralmuscleblocks(arrows)mrhe forelimbISnolV apparent.IC) E12.5 A numberof distinctmuscleblocks arenowapparentmrhepro""malforehmbIDI E13.5Musclefibers are revealedasfdrdisrallyasthe WflstlEI EI45.D,stmctmusclescan be seen In the foredrmwIth somemusclefiberse...rendmg mto rhe fmgers.Scale bars represent:(.4-C! -500Jlm:to and EJ - lmm.

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