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Normal development and neoplasia: the imprinting connection

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Figure

Fig. 1. A schematic view of thetranscriptionalpaireddifferentphicpromoter-specificprimed3IGF2unitwiththefourpromotersandpolymor-(C-Alnrepeatindicated.ThecDNAtranscriptsindicatedin the Figure were obtainedbyreversetranscribingtotalRNAatthe3'-endofthe(C-AJnrepeat.Each promoter-specificcDNAwas then singled out by PCR amplifi-cationusingpromoter-specificprimers with a common primer at the '-end of the (C-A)n repeat.
Figure 3 shows the allelewas heterozygous
Fig. 3. lGF2 is imprintedin the index patientof a familialform of theBeckwith-Wiedemannsyndromeand in first trimesterplacentaandembryo.RNA and DNA depict RNase protectionanalysis of total ceflu-Jar RNA and the PCR-amplifiedpolymorphic(C-AJnrepeat region wi
Fig. 4. Schematicsummaryof thepromoter-specificIGF2 alleleusage duringhuman liver development.Open or filledboxes depictopposite parental alleles, respectivelv.>,,

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