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Behavior of endodermal "button cells" during metamorphosis of ascidian larvae

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Figure

Fig. 1. Lateral view !leh)demonstratingsues of an 8-cellembryoof Phallusiamamillatatheorientationof blastomeresanddescendanttis-(fromdata of Orrolani.1957; Reverberiet al.
Fig. 3. Swimminglarvaeof Phal/usiamamillatastainedby FITC-conjugatedanti-tubulinantibodyandobservedundertheconfocalmicroscope.(A) The button cells (BO are alignedventrallyto the pharynx, betweenendodermand epidermis.(8) Higher magnificationof the line of r
Fig. 4. Metamorphosinglarvaeof Phallusiamamillatasectionsstainedby Nile blue sulphate.(A) Metachromaticbutton cells (arrows) mixed withnon-metachromatic.smaller mesenchymecells
Fig. 5. Metamorphosinglarvae stainedby FITC-conjugatedanti-tubulinantibody.(AI The button cells are scatteredand approachingthe epidermis.(B-C) Thebasesof the pseudopodsshow a bright anti-tubulin reaction

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