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Control of microtubule nucleating activity in the cytoplasm of maturing mouse oocytes

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Academic year: 2020

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Figure

Fig. 2. Doublefluorescencestainingof a taxol-treatedoocytefor (A)chromosomes,and(B) cytasters.withHoechstdye33258andantialphatubulinrespectively.Bar
Fig. 3. Ultrastructuralanalysis:(A! Control cortex.IB! Tightbundlesof rnlcrotubules(arrows)areradrating in the cortexof taxo/-treatedoocytes.while oocytestreatedwirhIBMX+taxolIC! showloosebundlesof microtubules(arrows),parallelto theplasmamembrane,butwithoutasterorganization.Bar,1,um.
Fig. 4, Anti alpha-tubulinimmunofluorescencestainingof mouseoocytenucleate (A,S,C,O) and anucleare IA',S',C',O'}fragmemscultured for 16h in taxa/-containingmediumIA,A',S,S')and in taxol+/BMX (C,C') or ta'w/+ TPA- (O,O'! containing media

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