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Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications

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Figure

Figure 1 Principles ofcence signals generally occurs at alonger wavelength. The shift be-tween excitation and emissionwavelengths is termed Stokesshift
Figure 2uorophores, thereby am-porter FPs) or fused in-frame with the geneticsequence of a protein-coding gene to create atagged version of the target protein (immunocally encoded avenue for the visualization ofcellular components
Figure 3 Numerical Aperture (NA),(A) Fluorophores are considered aspoint sources of light that emit pho-tons
Figure 4 Fluorescence microscopy technologies. Fluorescence imaging technologies can be classipoint detectors (PMTs)
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