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Neuronal Development in Caenorhabditis elegans Is Regulated by Inhibition of an MLK MAP Kinase Pathway

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Neuronal Development in

Caenorhabditis elegans

Is

Regulated by Inhibition of an MLK MAP

Kinase Pathway

Scott T. Baker,*,†Shane M. Turgeon,* Erik D. Tulgren,Jeanne Wigant,Omeed Rahimi,

Karla J. Opperman,* and Brock Grill*,1 *Department of Neuroscience, The Scripps Research Institute, Scripps Florida, Jupiter, Florida 33458, and†Department of Pharmacology, University of Minnesota, Minneapolis, Minnesota 55455

ABSTRACTWe show that loss-of-function mutations in kinases of theMLK-1pathway (mlk-1,mek-1, andkgb-1/jnk) function cell-autonomously in neurons to suppress defects in synapse formation and axon termination caused byrpm-1loss of function. Our genetic analysis also suggests that the phosphatasePPM-1, likeRPM-1, is a potential inhibitor of kinases in theMLK-1pathway.

I

NCaenorhabditis elegans, the ubiquitin ligaseRegulator ofPresynapticMorphology 1 (RPM-1) regulates neuro-nal development by inhibiting the DLK-1pathway (com-posed ofDLK-1,MKK-4, andPMK-3) (Nakataet al.2005). RPM-1 and the DLK-1 pathway regulate axon regenera-tion postdevelopmentally (Hammarlundet al. 2009; Yan et al. 2009). The MLK-1 pathway, which includes the kinases MLK-1, MEK-1, and KGB-1/JNK, also regulates axon regeneration (Nix et al. 2011). It remains unclear if RPM-1functions through the MLK-1pathway to regulate development.

ProteinPhosphataseMg/Mn2+-dependent 1 (PPM-1) and PPM-2negatively regulate theDLK-1pathway (Tulgrenet al. 2011; Baker et al.2014).PPM-2is regulated by RPM-1 and dephosphorylates full-lengthDLK-1(DLK-1L).PPM-1is likely to function lower in the DLK-1 pathway. It remains unclear whetherPPM-1andPPM-2regulate other signaling pathways in the neurons ofC. elegans.

Here, we show that mutations inmlk-1,mek-1, andkgb-1/ jnksuppress defects in synapse formation and axon termination caused byrpm-1loss of function (lf). These results suggest thatRPM-1might negatively regulate the MLK-1 pathway, which is consistent with our observation that transgenic

overexpression ofMLK-1orKGB-1caused axon termination defects. Furthermore, our results are consistent withPPM-1 negatively regulating theMLK-1pathway, in addition to inhib-iting theDLK-1pathway. In contrast, ourfindings suggest that PPM-2acts only on DLK-1L.

Results

Loss-of-function mutations in kinases of the MLK-1 pathway suppress defects in synapse formation caused by rpm-1 (lf)

The fly ortholog of RPM-1, called Highwire, functions through JNK to regulate synapse formation (Collins et al. 2006). It is unclear if RPM-1 functions through JNK to regulate synapse formation in worms, but studies on axon regeneration suggested that this might be a possi-bility (Nix et al. 2011). Hence, we assessed the genetic relationship between kinases of theMLK-1 pathway, in-cluding kgb-1/jnk and rpm-1 in the context of synapse formation.

Consistent with previous studies, the GABAergic motor neurons ofrpm-12/2mutants had synapse formation defects that were strongly, but incompletely, suppressed inrpm-12/2; dlk-12/2double mutants (Figure 1, A and B) (Nakataet al. 2005). In double mutants ofrpm-1withmlk-1,mek-1, orkgb-1, significant but modest suppression occurred (Figure 1, A and B).rpm-12/2mlk-12/2;kgb-12/2triple mutants did not show increased suppression, demonstrating that mlk-1

and kgb-1 function in the same genetic pathway (Figure Copyright © 2015 by the Genetics Society of America

doi: 10.1534/genetics.114.170589

Manuscript received September 5, 2014; accepted for publication October 21, 2014; published Early Online October 22, 2014.

Supporting information is available online athttp://www.genetics.org/lookup/suppl/ doi:10.1534/genetics.114.170589/-/DC1.

1Corresponding author: The Scripps Research Institute, 130 Scripps Way, Jupiter, FL

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1B). rpm-12/2 mlk-12/2; dlk-1 triple mutants showed a small, but significant, increase in suppression, consistent withmlk-1anddlk-1functioning in partially redundant path-ways (Figure 1B).

These results are consistent withRPM-1regulating syn-apse formation by inhibiting both theDLK-1and theMLK-1 pathways. Notably, thesefindings do not rule out the possi-bility that kinases in theMLK-1pathway function in a paral-lel genetic pathway torpm-1.

Mutations in kinases of the MLK-1 pathway suppress axon termination defects caused by rpm-1 (lf)

Two types of axon termination defects are present in the PLM mechanosensory neurons ofrpm-12/2mutants (Schaefer et al.2000; Grillet al.2007; Tulgrenet al.2011): (1) severe, highly penetrant defects in which an axon overextends and hooks toward the ventral cord, referred to as“hook defects”

(Figure 2, A and B) and (2) rarely observed, milder defects in which an axon overextends but fails to hook ventrally, referred to as “overextension defects.” Hook defects were strongly suppressed inrpm-12/2;dlk-12/2double mutants (Figure 2B). In double mutants ofrpm-1withmlk-1,mek-1, orkgb-1, the frequency of hook defects was moderately sup-pressed, while the expressivity of less severe overextension defects was increased (Figure 2, A and B). These effects were not increased in rpm-12/2 mlk-12/2; kgb-12/2

triple mutants (Figure 2B). Transgenic expression of MLK-1specifically in the mechanosensory neurons rescued the suppression in rpm-12/2 mlk-12/2 double mutants (Figure 2C).

These results show thatmlk-1,mek-1, andkgb-1function cell-autonomously in the same genetic pathway to suppress

rpm-1 (lf). These findings are also consistent with RPM-1 negatively regulating theMLK-1pathway.

Figure 1 Loss-of-function mutations in kinases of the MLK-1 pathway sup-press synapse formation defects in rpm-1mutants. (A) Defects in synapse formation in the GABAergic motor neurons were analyzed using a trans-gene, juIs1 (Punc-25SNB-1::GFP), and

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Figure 2 Axon termination defects inrpm-1mutants are suppressed by loss of function inmlk-1,mek-1, orkgb-1. (A) A schematic of the mechanosensory neurons ofC. elegans(adapted from Bakeret al.2014). Axon termination of the PLM mechanosensory neurons was analyzed using a transgene,muIs32 (Pmec-7GFP), and epifluorescent microscopy under340 magnification. Shown are representative images of a more severe axon termination defect in

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fsn-1 (lf) is suppressed by mutations in kinases of the DLK-1 pathway, but not in kinases of the MLK-1 pathway

RPM-1functions as a complex with the F-box proteinFSN-1 to regulate PLM axon termination (Liao et al. 2004; Grill et al.2007). Axon termination defects infsn-12/2mutants were suppressed in fsn-12/2; dlk-12/2 and fsn-12/2; pmk-32/2 double mutants (Figure 2D) (Bakeret al. 2014). In contrast, the frequency of axon termination defects remained unchanged infsn-12/2;mlk-12/2orfsn-12/2;kgb-12/2

double mutants (Figure 2D). These findings are consistent withFSN-1inhibiting theDLK-1pathway, but not theMLK-1 pathway.

Excess MLK-1 pathway function impairs axon termination

One explanation for whyrpm-1(lf) is suppressed by mutations in the MLK-1 pathway is that rpm-1 mutants have excess, unchecked MLK-1 pathway function. To test this hypothesis, we generated transgenic animals with extrachromsomal arrays that expressed different kinases in theDLK-1andMLK-1 path-ways. To assess the range of defects that might be caused by kinase overexpression, we generated arrays with varying levels of DNA encoding different kinases. ForMLK-1andKGB-1, we observed primarily less severe overextension defects and very low, but significant, levels of hook defects at higher concentra-tions (Figure 2, E and F). Similar results were observed with

overexpression of PMK-3 (Figure 2, E and F). In contrast, overexpression of DLK-1L caused more severe hook defects, which occurred with increasing frequency as the concentra-tion of DLK-1L increased (Figure 2, E and F) (Tulgrenet al. 2011; Baker et al. 2014). We did not analyze MKK-4, but previous work showed that MKK-4 overexpression causes an intermediate frequency of hooks (Bakeret al.2014). These results provide further support for the model that RPM-1 negatively regulates theMLK-1pathway.

Analysis of PPM-1 and PPM-2 function on the MLK-1 pathway

The phosphatasesPPM-1andPPM-2negatively regulate the DLK-1 pathway (Tulgren et al. 2011; Baker et al. 2014). Using a combination of suppressor genetics and transgenics, we tested whetherPPM-1and/orPPM-2affect the function of theMLK-1 pathway.

Previously, we found that ppm-22/2mutants had very low penetrance hook defects, which were completely sup-pressed in ppm-22/2; dlk-12/2 double mutants (Baker et al. 2014). In contrast, hook defects were not suppressed inppm-22/2;mlk-12/2orppm-22/2;kgb-12/2double mutants (Figure 3A). In the case ofppm-12/2mutants that lack hook defects, we utilized a glo-4(lf) sensitizing back-ground that is enhanced byppm-1(lf) (Figure 4A) (Tulgren et al. 2011). The enhanced frequency of hooks present in

ppm-12/2 glo-42/2 double mutants was suppressed in

times larger than other constructs. Transgenic animals were generated by microinjecting a mixture of PCR product encoding the indicated construct, 50 ng/ml of Pttx-3::RFP (coinjection marker) and 50 ng/ml of pBluescript. Injection conditions and genotypes for all transgenes are annotated in supporting information,Table S1. Averages are shown for data collected fromfive to eight independent counts of 20–30 PLM neurons from young adult worms grown at 23°. For transgenic genotypes, averages shown are data-pooled from four or more independent lines. Alleles used includedrpm-1 (ju44),fsn-1 (gk429),dlk-1 (ju476),pmk-3 (ok169),mlk-1 (ok2471),mek-1 (ks54), andkgb-1 (um3). Error bars represent the standard error of the mean, and significance was determined using an unpaired Student’st-test.***P,0.001;**P,0.01;*P,0.05; ns, not significant.

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ppm-12/2glo-42/2mlk-12/2andppm-12/2glo-42/2; kgb-12/2triple mutants (Figure 4A). In contrast, hook defects in glo-42/2 mutants were not suppressed in glo-42/2 mlk-12/2andglo-42/2;kgb-12/2double mutants.

Next, we analyzed whether PPM-1 and PPM-2 regulate theMLK-1pathway in the context of transgenic overexpres-sion experiments. As shown in Figure 3B, transgenic over-expression ofMLK-1orKGB-1resulted in axon termination defects, and coexpression ofPPM-2 in the same transgenic arrays did not affectMLK-1orKGB-1functional efficacy. In

contrast, transgenic coexpression of PPM-1significantly re-duced the defects caused by overexpression of MLK-1 and KGB-1 (Figure 4B). Consistent with the previousfindings on DLK-1L (Tulgrenet al.2011), defects caused by trans-genic overexpression of PMK-3were reduced by transgenic coexpression ofPPM-1(Figure 4B). Importantly, catalytically inactivePPM-1D246Ndid not reduce defects caused by over-expression of MLK-1 or KGB-1. Thus, PPM-1 phosphatase activity regulates excess MLK-1 pathway function, and the reduction caused by coexpression ofPPM-1is not an indirect

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consequence of incorporating a second gene into extrachro-mosomal arrays (Figure 4B).

Collectively, these results are consistent with PPM-1 phosphatase activity inhibiting both the DLK-1 and the MLK-1 pathways. In contrast, these results suggest that PPM-2is more specific for DLK-1L and not capable of reg-ulating the MLK-1pathway.

Discussion

RPM-1 is an important signaling molecule that regulates neuronal development through multiple mechanisms (Grill et al.2007, 2012; Bakeret al. 2014; Tulgrenet al.2014), in-cluding ubiquitination and inhibition of DLK-1L (Nakataet al. 2005). Our genetic suppressor analysis and transgenic results suggest thatRPM-1also negatively regulates theMLK-1 path-way during development. Ourfindings are consistent with a pre-vious study, which showed thatMLK-1levels are increased in the neurons ofrpm-1(lf) mutants (Nixet al.2011). One simple explanation for these findings is that RPM-1 ubiquitinates MLK-1, which results inMLK-1degradation and inhibition of the MLK-1 pathway (Figure 4C). However, an alternative explanation for our results is that theMLK-1pathway functions in parallel to RPM-1. Because mutations in kinases of the DLK-1pathway are stronger suppressors ofrpm-1(lf) than mutations in kinases of theMLK-1pathway, it is likely that RPM-1 functions primarily through the DLK-1 pathway and secondarily through the MLK-1 pathway (Figure 1 and Figure 2).

Our results are consistent with the PPM-1 phosphatase representing a further, conserved negative regulatory mech-anism imposed on the DLK-1and MLK-1pathways (Figure 4C). In contrast, we found no evidence thatPPM-2regulates the MLK-1 pathway. Therefore, taking prior work into account,PPM-2is likely to be a relatively specific mechanism for restraining DLK-1L activity (Bakeret al.2014).

During neuronal development, JNK and p38 MAP kinases mediate the function of DrosophilaHighwire and mammalian Phr1 (Collinset al.2006; Lewcocket al.2007; Huntwork-Rodriguez et al.2013; Klinedinst et al. 2013). Given prior work and ourfindings here, it is increasingly likely that the Pam/Highwire/RPM-1protein family generally regulates two MAP kinase pathways exemplified by theDLK-1 andMLK-1pathways inC. elegans.

Acknowledgments

We thank Michael Bastiani and Paola Nix for providing C. elegansstrains and for helpful discussions. Strains were also provided by theC. elegansGenetics Center. B.G. was funded by National Institutes of Health grant R01 NS072129 and National Science Foundation grant IOS-1121095.

Literature Cited

Baker, S. T., K. J. Opperman, E. D. Tulgren, S. M. Turgeon, W. Bienvenut et al., 2014 RPM-1 uses both ubiquitin ligase and phosphatase-based mechanisms to regulate DLK-1 during neu-ronal development. PLoS Genet. 10: e1004297.

Collins, C. A., Y. P. Wairkar, S. L. Johnson, and A. Diantonio, 2006 Highwire restrains synaptic growth by attenuating a MAP kinase signal. Neuron 51: 57–69.

Grill, B., W. V. Bienvenut, H. M. Brown, B. D. Ackley, M. Quadroni et al., 2007 C. elegans RPM-1 regulates axon termination and synaptogenesis through the Rab GEF GLO-4 and the Rab GTPase GLO-1. Neuron 55: 587–601.

Grill, B., L. Chen, E. D. Tulgren, S. T. Baker, W. Bienvenutet al., 2012 RAE-1, a novel PHR binding protein, is required for axon termination and synapse formation in Caenorhabditis elegans. J. Neurosci. 32: 2628–2636.

Hammarlund, M., P. Nix, L. Hauth, E. M. Jorgensen, and M. Bastiani, 2009 Axon regeneration requires a conserved MAP kinase path-way. Science 323: 802–806.

Huntwork-Rodriguez, S., B. Wang, T. Watkins, A. S. Ghosh, C. D. Pozniak et al., 2013 JNK-mediated phosphorylation of DLK suppresses its ubiquitination to promote neuronal apoptosis. J. Cell Biol. 202: 747763.

Klinedinst, S., X. Wang, X. Xiong, J. M. Haenfler, and C. A. Collins, 2013 Independent pathways downstream of the Wnd/DLK MAPKKK regulate synaptic structure, axonal transport, and in-jury signaling. J. Neurosci. 33: 12764–12778.

Lewcock, J. W., N. Genoud, K. Lettieri, and S. L. Pfaff, 2007 The ubiquitin ligase Phr1 regulates axon outgrowth through modulation of microtubule dynamics. Neuron 56: 604–620.

Liao, E. H., W. Hung, B. Abrams, and M. Zhen, 2004 An SCF-like ubiquitin ligase complex that controls presynaptic differentia-tion. Nature 430: 345–350.

Nakata, K., B. Abrams, B. Grill, A. Goncharov, X. Huang et al., 2005 Regulation of a DLK-1 and p38 MAP kinase pathway by the ubiquitin ligase RPM-1 is required for presynaptic devel-opment. Cell 120: 407420.

Nix, P., N. Hisamoto, K. Matsumoto, and M. Bastiani, 2011 Axon regeneration requires coordinate activation of p38 and JNK MAPK pathways. Proc. Natl. Acad. Sci. USA 108: 10738 10743.

Schaefer, A. M., G. D. Hadwiger, and M. L. Nonet, 2000 rpm-1, a conserved neuronal gene that regulates targeting and synapto-genesis in C. elegans. Neuron 26: 345–356.

Tulgren, E. D., S. T. Baker, L. Rapp, A. M. Gurney, and B. Grill, 2011 PPM-1, a PP2Ca/bphosphatase, regulates axon termi-nation and synapse formation inCaenorhabditis elegans. Genet-ics 189: 1297–1307.

Tulgren, E. D., S. M. Turgeon, K. J. Opperman, and B. Grill, 2014 The Nesprin family member ANC-1 regulates syn-apse formation and axon termination by functioning in a pathway with RPM-1 and beta-catenin. PLoS Genet. 10: e1004481.

Yan, D., Z. Wu, A. D. Chisholm, and Y. Jin, 2009 The DLK-1 kinase promotes mRNA stability and local translation in C. elegans synapses and axon regeneration. Cell 138: 1005 1018.

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GENETICS

Supporting Information http://www.genetics.org/lookup/suppl/doi:10.1534/genetics.114.170589/-/DC1

Neuronal Development in

Caenorhabditis elegans

Is

Regulated by Inhibition of an MLK MAP

Kinase Pathway

Scott T. Baker, Shane M. Turgeon, Erik D. Tulgren, Jeanne Wigant, Omeed Rahimi, Karla J. Opperman, and Brock Grill

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Table S1

* Alleles used are

mlk-1(ok2471)

and

rpm-1(ju44)

^ Plasmids were injected, unless noted otherwise

+ Genomic DNA was used, unless noted otherwise

Figure #

Injection Conditions^+

Genotype*

2C P

mec-7

MLK-1 (pBG-GY554) (5ng/µL)

muIs32; mlk-1 rpm-1

2C P

mec-7

mCherry (pBG-GY258) (5ng/µL)

muIs32; mlk-1 rpm-1

2F P

rgef-1

DLK-1 cDNA (pBG-57) (2.5ng/µL PCR Product)

muIs32

2F P

rgef-1

DLK-1 cDNA (pBG-57) (5ng/µL PCR Product)

muIs32

2F P

rgef-1

DLK-1 cDNA (pBG-57) (10ng/µL PCR Product)

muIs32

2F P

rgef-1

PMK-3 (pBG-GY449) (2.5ng/µL PCR Product)

muIs32

2F P

rgef-1

PMK-3 (pBG-GY449) (5ng/µL PCR Product)

muIs32

2F, 4B

P

rgef-1

PMK-3 (pBG-GY449) (10ng/µL PCR Product)

muIs32

2F P

rgef-1

PMK-3 (pBG-GY449) (20ng/µL PCR Product)

muIs32

2F, 3B, 4B

P

rgef-1

MLK-1 (pBG-GY356) (5ng/µL PCR Product)

muIs32

2F P

rgef-1

MLK-1 (pBG-GY356) (10ng/µL PCR Product)

muIs32

2F P

rgef-1

MLK-1 (pBG-GY356) (20ng/µL PCR Product)

muIs32

2F P

rgef-1

KGB-1 (pBG-GY357) (2.5ng/µL PCR Product)

muIs32

2F, 3B, 4B

P

rgef-1

KGB-1 (pBG-GY357) (5ng/µL PCR Product)

muIs32

2F P

rgef-1

KGB-1 (pBG-GY357) (10ng/µL PCR Product)

muIs32

2F P

rgef-1

KGB-1 (pBG-GY357) (20ng/µL PCR Product)

muIs32

3B P

rgef-1

MLK-1 (pBG-356) (5ng/µL PCR Product)

P

mec-7

PPM-2 (pBG-GY121) (5ng/µL)

muIs32

3B P

rgef-1

KGB-1 (pBG-357) (10ng/µL PCR Product)

P

mec-7

PPM-2 (pBG-GY121) (5ng/µL)

muIs32

4B P

rgef-1

MLK-1 (pBG-356) (5ng/µL PCR Product)

P

mec-7

PPM-1 cDNA (pBG-GY163) (2ng/µL)

muIs32

4B P

rgef-1

MLK-1 (pBG-356) (5ng/µL PCR Product)

P

mec-7

PPM-1

D246N

(pBG-GY202) (2ng/µL)

muIs32

4B P

rgef-1

KGB-1 (pBG-357) (10ng/µL PCR Product)

P

mec-7

PPM-1 cDNA (pBG-GY163) (2ng/µL)

muIs32

4B P

rgef-1

KGB-1 (pBG-357) (10ng/µL PCR Product)

P

mec-7

PPM-1

D246N

cDNA (pBG-GY202) (2ng/µL)

muIs32

4B P

rgef-1

PMK-3 (pBG-GY449) (10ng/µL PCR Product)

P

mec-7

PPM-1 cDNA (pBG-GY163) (2ng/µL)

muIs32

4B P

rgef-1

PMK-3 (pBG-GY449) (10ng/µL PCR Product)

P

mec-7

PPM-1

D246N

cDNA (pBG-GY202) (2ng/µL)

Figure

Figure 1 Loss-of-function mutationsepimagnipressed by loss of function inmek-1titation of synapse formation defects inGABAergic motor neurons for the indi-cated genotypes
Figure 2 Axon termination defects inshown for a PLM neuron from transgenic animals overexpressing the indicated kinases using a pan-neuronal promoter (Pdefect is highlighted with an arrowhead, and less severe overextension defects are highlighted with arro
Figure 3 The PP2C phosphatase PPM-2 does not regulate the MLK-1 pathway. (A) Quantitation of PLM axon termination defects (hook) for the***indicated genotypes
Figure 4 PPM-1 inhibits the MLK-1 and DLK-1 pathways. (A) Quantitation of PLM axon termination defects (hook) for the indicated genotypes
+2

References

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