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Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing based quantification

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Figure

Fig. 1 REcount enables accurate and precise measurements of plasmid pools.Analogous measurements of the same plasmid pool shown in panelexpected values (5% per construct) when the plasmid pool is measured using REcount, and varying cycles of PCR amplificat
Fig. 2 Multiplexing of REcount measurements using orthogonal restriction enzymes. a Plasmids containing REcount constructs flanked byorthogonal restriction enzyme cut sites
Fig. 3 Illumina size standards allow measurement of sequencer-specific size biases.run variability of multiple MiSeq runs ( a Design of REcount-based Illumina size standard constructs.Each standard construct contains a normalization barcode, as well as a b
Fig. 4 Instrument-specific size biases have minimal effect on RNA-sequencing data. a Fragment size distributions for an RNA-Seq librarysequenced on the NovaSeq and the NextSeq
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