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GUCY2C lysosomotropic endocytosis delivers immunotoxin therapy to metastatic colorectal cancer

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Academic year: 2020

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Figure

Figure 1: Intracellular localization of GUCY2C in intestinal epithelial and colorectal cancer cells
Figure 2: Lysosomotropic endocytosis of GUCY2C. (A) GUCY2C immunofluorescence (green) co-localized (yellow) with lysosomes (red) in colons from GUCY2C+/+, but not GUCY2C-/-, mice (60x magnification)
Figure 3: Lysosomotropic endocytosis of GUCY2C is clathrin-dependent. (A) Representative single cell images showing internalized GUCY2C (green) detected as in A-C in cells with stably transfected control (I) or clathrin-specific (II) shRNA
Figure 4: GUCY2C-targeted immunotoxin delivers dgRTA specifically to GUCY2C. (A) The design of cleavable ITsmpt conjugates of dgRTA and GUCY2C-specific mAb
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