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Characterization of background noise in capture based targeted sequencing data

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Figure

Fig. 1 Base call quality in targeted deep sequencing data.bases.box-plotted for PBL ( a The density plot visualizes Phred base quality score distribution of background and total b, c After the removal of bases with a quality score <30, the average base quality scores (i.e., total and for each of the four nucleotides) wasb) and plasma (c) DNA samples
Fig. 2 Characterization of background errors in targeted deep sequencing data. a The background allele frequencies from plasma and PBL DNAsamples were box-plotted (n = 19 for each group)
Fig. 3 The effect of DNA fragmentation on the background allele frequency.generated using genomic input DNA fragmented under various conditions
Fig. 4 Characterization of the DNA break point. a Fold changes in error rates across all substitution classes were compared between PBL andplasma DNA samples
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