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Rapid, low input, low bias construction of shotgun fragment libraries by high density in vitro transposition

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Figure

Figure 1 Methods for constructing in vitro fragment libraries. (a) In the conventional protocol, mechanical or endonuclease fragmentation isfollowed by end-polishing, A-tailing, adaptor ligation and PCR
Figure 2 Schematic of steps associated with different library preparation methods. Transposase-catalyzed adaptor insertion significantlyreduces the number of steps and time associated with library construction (green path).
Figure 3 Comparison of coverage bias. (a) Coverage distribution across the E. coli genome with transposase (blue), sonication (red), andendonuclease (green) methods (solid lines) and replicates (dotted lines), normalized for total sequencing depth
Figure 4 Insert size showing steric hindrance. (a) Insert size was generated from libraries spiked into a paired-end 101 bp run resulting in alarge proportion of reads reading into the adaptor sequence
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