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Role of microtubules in the rapid regulation of renal phosphate transport in response to acute alterations in dietary phosphate content

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Academic year: 2020

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Figure

Figure 1. Effect of acute (2 h)
Figure 3. Immunohistochemistry, demonstrating the effect of colchicine on rapid upregulation of apical NaPi-2 protein and on microtubule in-tegrity
Figure 4. Histochemical detection of F-actin with rhodamine-conjugated phalloidin, revealing no effect of colchicine on the microfilamentous cytoskeleton
Figure 5. Effect of acute (2 h) low phosphate (0.1% Pi) diet on renal cortical BBM sodium gradient–dependent phosphate transport (Na-Pi cotransport) activity and type II renal Na-Pi cotransporter (NaPi-2) protein abundance in (top) vehicle (saline) treated rats and (bottom) colchi-cine-treated rats (1 mg/kg body wt, given intraperitoneally 4 h before acute low Pi diet).
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