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The deubiquitinating enzyme USP17 is essential for GTPase subcellular localization and cell motility

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Figure

Figure 1 | USP17 expression is required for chemotaxis. Human PBmCs (a,b) or HeLa cells (c,d) were stimulated with CXCL12 (100 ng ml − 1) for the indicated times
Figure 2 | USP17 depletion inhibits chemokinesis. Hut78 T cells were cotransfected with either scrambled shRnA or usP17 shRnA1 together with pmAX-GFP to determine transfected cells
Figure 3 | USP17 shRNA alters cell morphology. HeLa cells transfected with scrambled shRnA were left unstimulated (a) or stimulated with CXCL12 (100 ng ml − 1) (b)
Figure 5 | Knockdown of USP17 inhibits GTPase localization and activation. HeLa cells were transfected with scrambled shRnA or usP17 shRnA1 along with GFP-tagged Cdc42 (a), Rac1 (b) and RhoA (c)
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