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Role of Alström syndrome 1 in the regulation of blood pressure and renal function

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Figure

Figure 1. Interaction of ALMS1 with C2-NKCC2. (A) Representation of C2-NKCC2 domain (pink) and unique peptides corresponding to ALMS1 (red) picked up by liquid chromatography–mass spectrometry in glutathione-s-transferase (GST) pull-down assay using GST-ca
Figure 2. Expression and colocalization of ALMS1 and NKCC2 in rat TAL. (A) Representative immunoblot of ALMS1 indicating ALMS1 protein expression in rat thick ascending limb (TAL) corresponding to 230 kDa protein; n = 4
Figure 3. Generation of ALMS1-KO rats and their genotyping. (A) Scheme showing deletion of 17-bp sequence from exon 1 of ALMS1 gene by zinc finger nucleases leading to premature stop codon in exon 2, resulting in KO of ALMS1 in rats
Table 1. Measurement of physiological parameters in 6–12 week old WT and ALMS1-KO rats
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