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One-Step RT-qPCR assay for detection and quantification of equine infectious anemia virus in-vitro

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Academic year: 2020

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Figure

Figure 1:  After the midiprep process, the material was quantified using a Low DNA Mass ladder (2)
Figure 2:  Preparation of the template DNA to RNA synthesis. The band that ran the slowest corresponds to the linearized plasmid (2 and 3) and the band that ran fastest corresponds to the plasmid in its circular form (1)
Figure 4:  Construction of the control curve. Representation the amplification curves of the standard plasmid DNA curve
Figure 5:  Schematic representation of the standard curve containing the log10 dilution points of plasmid DNA (blue) and tested dilution points of the two synthetic EIAV RNAs (red), which amplified within the linearity of the standard curve

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