Expression. Labeling. Crystallization. Purification. 伯 森 生 物 科 技 股 份 限 公 司 Blossom Biotechnologies Inc. P. 8 P. 3 P. 5 P. 6 P. 6 P. 2 P. 7 P.

伯森蛋白質研究產品專刊

伯森蛋白質研究產品專刊

伯森蛋白質研究產品專刊

Protein

Research

Product

Catalog

Protein

Research

Product

Catalog

Protein

Research

Product

Catalog

Expression

LEXSY Protein

Expression System

P. 2

Purification

Affinity

Size Exclusion

Ion Exchange

FPLC Accessories

P. 3

P. 5

P. 6

P. 6

Crystallization

Screening Kit

Crystallography

Starter Kit

Detergents

P. 7

P. 7

P. 7

Labeling

FluoroSpin Protein

Labeling and

Purification Kit

P. 8

Cultured Leishmania tarentolae cells expressing green uorescent protein.

JB-EGE-1300 JB-EGE-1410 JB-EGE-1420 JB-EGE-2002-15

LEXSYcon2 Expression Kit, for constitutive protein expression

LEXSinduce3 Expression Kit, for inducible expression from integrative constructs LEXSinduce4 Expression Kit, for inducible expression from episomal constructs LEXSY in vitro Translation Kit, for plasmid based cell-free protein synthesis Cat. No. Product Name

LEXSY Protein Expression System

LEXSY

是德商

Jena Bioscience

的獨家

蛋白質表現系統，以單細胞原蟲為宿

主，兼具了真核生物系統以及大腸桿菌

系統的優點，是您大量表現蛋白的最佳

選擇！

Bacteria Yeast

e.g. Pichia e.g. Sf9/21Insect cells LEXSY Mammaliancells

LEXSY performs mammalian type of glycosylation

(Breitling et al. 2002)

LEXSY strains grow higher densities

than insect and mammalian cells

(Fritsche et al. 2007)

LEXSY grows faster

than insect and mammalian cells

LEXSY

完美克服其他蛋⽩質表現系統常⾒問題

Yeast

Bacteria Insect &Mammalian cells

§⽣產蛋⽩質無後修飾 §無法摺疊成正確的構形 §具包涵體 §後修飾與哺乳⽣物差異⼤ §細胞不易破壞,抽取蛋⽩質困難 §表現量低 §⽣⾧速率緩慢 §培養液價格昂貴

LEXSY特點：

þ不具包涵體(inclusionbody) þ後修飾(post-translationalmodification)近似哺乳⽣物細胞 þ操作流程條件穩定 þ表現量⾼，產量⼤ þ不具⽣物毒性(Biosafetylevel1)

Purification

FPLC

純化膠體與管柱

伯森公司擁有完整的

FPLC

純化商品，包含親和性層析、膠體過濾、

離子交換層析等，提供來自德國、瑞典與日本的優良產品。各式產品

均擁有極高性價比，歡迎進一步索取相關資料及免費試用服務！

Affinity

親和性層析

His-tag 重組蛋白純化

His-tag 重組蛋白偵測

膠體 重⼒流 磁珠 預注管柱 ABT-6BCL-NTANi-10 ABT-6BCL-NTANi-100 ABT-6BCL-NTANi-500 CU-31103 CU-31105 ABT-6BCL-QHNi-C8 ABT-6BCL-QHNi-C5 MF-HIS-3000-2ML MF-HIS-3000-5ML BW-45-655-103 BW-45-655-104 BW-45-655-107 BW-45-655-108 ABT-AF6NI-CTG5-2 ABT-AF6NI-CTG5-5

NICKEL NTA Agarose Resin, 10 ml NICKEL NTA Agarose Resin, 100 ml NICKEL NTA Agarose Resin, 500ml PureCube Ni-NTA Agarose, 10 ml PureCube Ni-NTA Agarose, 50 ml

His-COLUMN HIGH Density NICKEL (8x1 ml) HisXL-COLUMN HIGH Density NICKEL (5x5 ml) Qbeads-NTA-Ni (2x1 ml) Qbeads-NTA-Ni (5x1 ml) BabyBio Ni-NTA (5x1 ml) BabyBio Ni-NTA (10x1 ml) BabyBio Ni-NTA (5x5 ml) BabyBio Ni-NTA (10x5 ml)

NICKEL Afnity Cartridges 5ml (2x5 ml) NICKEL Afnity Cartridges 5ml (5x5 ml)

NI Sepharose 6FF NI Sepharose 6FF NI Sepharose 6FF NI Sepharose HP NI Sepharose HP

His Mag Sepharose His Mag Sepharose Histrap HP Histrap HP Histrap HP Histrap HP Histrap FF Histrap FF

Type Cat. No. Product Name GE 對應商品

JB-PS110 His Detection Kit

Detection of poly-histidine tagged proteins, ready-to-use solution Up to 3 x 3 reactions

Cat. No. Product Name

His Detection Kit

l

快速偵測

His Tag

重組蛋白純化結果

l

不需

Blocking, Washing

等步驟

l

偵測極限可達

0.1 pmole

l

肉眼即可觀察，也可再使用儀器定量

1 hr Transfer 1-15 hr Block 30' Wash 1-2 hr 1st Ab 30' Wash 1-2 hr 2nd Ab 30' Wash 5' Develop 1 hr Fix Gel TOTAL 6-21 hr 20' Wash 1 hr Stain 20' Wash TOTAL 3 hr 1 hr 30'-90' In-Gel Stain His Western Blot >5XFasterThanWesternBlot! TOTAL

GST-tag 重組蛋白純化

抗體純化

其它親和性層析純化

膠體 磁珠 預注管柱 Tag切除 ABT-4B-GLU-10 ABT-4B-GLU-100 CU-32103 CU-32105 CU-32205 CU-32303 CU-32305 CU-32307 BML-SE363-1000

Glutathione Agarose Resin, 10 ml Glutathione Agarose Resin, 100ml PureCube Glutathione Agarose, 10 ml PureCube Glutathione Agarose, 50 ml PureCube Glutathione MagBeads (5 ml) PureCube Glutathione Cartridge (5x1 ml) PureCube Glutathione Cartridge (1x5 ml) PureCube Glutathione Cartridge (5x5 ml) Thrombin

Type Cat. No. Product Name

Glutathione Sepharose 4B Glutathione Sepharose 4B Glutathione Sepharose HP Glutathione Sepharose HP GSTrap HP GSTrap HP GSTrap HP GE 對應商品 膠體 磁珠 預注管柱 BB-02-01PA-01 BB-02-01PG-01 ABT-4BCLPL-R5 MF-PRA-3000-2ML MF-PRA-3000-5ML MF-PRA-3000-10ML MF-PRG-3000-2ML MF-PRG-3000-5ML MF-PRG-3000-10ML BW-45-605-102 BW-45-605-103 BW-45-605-106 BW-45-605-107 Protein A agarose, 10 ml Protein G agarose, 10 ml Protein L agarose, 5ml Qbeads-Protein A, 2ml Qbeads-Protein A, 5ml Qbeads-Protein A, 10ml Qbeads-Protein G, 2ml Qbeads-Protein G, 5ml Qbeads-Protein G, 10ml BabyBio proteinA (5x1 ml) BabyBio proteinA (10x1 ml) BabyBio proteinA (5x5 ml) BabyBio proteinA (10x5 ml)

Type Cat. No. Product Name

nProtein A Sepharose Protein G Sepharose Protein L sepharose

Protein A Mag Sepharose Xtra 5 Protein A Mag Sepharose Xtra 5 Protein A Mag Sepharose Xtra 5 Protein G Mag Sepharose Xtra 5 Protein G Mag Sepharose Xtra 5 Protein G Mag Sepharose Xtra 5 Hitrap protein A HP Hitrap protein A HP Hitrap protein A HP Hitrap protein A HP GE 對應商品 膠體 磁珠 ABT-6RRF-STV-5 CHI-676943324 CU-50303 CU-33102 MF-STA-3000-5ML MF-STA-3000-10ML MF-DEX-3000-5ML MF-COO-3000-5ML MF-NHH-3000-5ML Streptavidin Agarose, 5ml Cellune sulfate

PureCube NHS Activated Agarose, 10ml PureCube Rho1D4 Agarose, 5ml Qbeads-Streptavidin

Qbeads-Streptavidin Qbeads-Hydroxyl Qbeads-Carboxyl Qbeads-Amine

Type Cat. No. Product Name

Streptavidin Sepharose Heparin Sepharose NHS Sepharose

Streptavidin Mag Sepharose Streptavidin Mag Sepharose GE 對應商品

Purification

Size Exclusion

膠體過濾

去鹽與置換緩衝液

Gel

Filtration

膠體過濾

離⼼ 膠體 重⼒流 CP-0201-Z025.0-001 CP-0201-Z100.0-001 CP-0205-Z025.0-001 CP-0205-Z100.0-001 CP-0202-Z025.0-001 CP-0202-Z100.0-001 TM-0102-E100.0-001 TM-0102-E500.0-001 TM-0105-E100.0-001 TM-0105-E500.0-001 TM-0106-E100.0-001 CP-0109-Z050.0-001 CP-0104-Z050.0-001 CP-0105-Z025.0-001

Z-50 MINI Spin Columns, 25pk Z-50 MINI Spin Columns, 100pk Z-25 MINI Spin Columns, 25pk Z-25 MINI Spin Columns, 100pk SEQ Z-50 MINI Spin Columns, 25pk SEQ Z-50 MINI Spin Columns, 100pk Zetadex-25 Fine, 100g Zetadex-25 Fine, 500g Zetadex-50 Fine, 100g Zetadex-50 Fine, 500g Zetadex-50, 100g N2 Gravity Columns (1-200 µl), 50pk N10 Gravity Columns (0.5-1 ml), 50pk N25 Gravity Columns (1-2.5 ml), 25pk

Type Cat. No. Product Name

Microspin G-50 columns Microspin G-50 columns SpinTrap G-25 SpinTrap G-25 AUTOSEQ G-50 AUTOSEQ G-50 Sephadex G-25 ne Sephadex G-25 ne Sephadex G-50 ne Sephadex G-50 ne Sephadex G-50 DNA Grade MiniTrap G-25 PD-10 Desalting Columns PD-10 Desalting Columns GE 對應商品 膠體 EMP-TM-0501-E100.0-001 EMP-TM-0501-E500.0-001 ABT-A-1020S-1000 ABT-A-1040S-1000 ABT-A-1060S-1000 ABT-A-1021S-1000 ABT-A-1041S-1000 ABT-A-1061S-1000 Zetadex 20-LH, 100g Zetadex 20-LH, 500g 2% B Agarose Bead 4% B Agarose Bead 6% B Agarose Bead 2% BCL Agarose Bead 4% BCL Agarose Bead 6% BCL Agarose Bead

Type Cat. No. Product Name

Sephadex LH-20 Sephadex LH-20 Sepharose 2B Sepharose 4B Sepharose 6B Sepharose CL-2B Sepharose CL-4B Sepharose CL-6B GE 對應商品

Ion Exchange

離子交換

膠體 預注管柱 CHI-675982327 CHI-2120051 CHI-675980327 CHI-675983327 CHI-1990751 CHI-2120051 CHI-1980551 CHI-1980051 BW-45-100-107 BW-45-200-103 BW-45-200-107 BW-45-150-103 BW-45-150-107 Cellune Q, 100ml Cellune S, 100ml Cellune A, 100ml Cellune C, 100ml Cellune Q (5x1 ml) Cellune S (5x1 ml) Cellune A (5x1 ml) Cellune C (5x1 ml) BabyBio Q (5x5 ml) BabyBio S (5x1 ml) BabyBio S (5x5 ml) BabyBio DEAE (5x1 ml) BabyBio DEAE (5x5 ml)

Type Cat. No. Product Name

Q Sepharose SP Sepharose DEAE Sepharose CM Sepharose Hitrap Q FF Hitrap SP FF Hitrap DEAE FF Hitrap CM FF Hitrap Q HP Hitrap S HP Hitrap S HP Hitrap DEAE HP Hitrap DEAE HP GE 對應商品

FPLC Accessories

純化配件

空管柱 磁座 C-50 CXL-50 MC-25 MC-100 ABT-FPLC8-3 ABT-FPLC30-2 ABT-FPLC45-2 ABT-FPLC80-1 O-MAGRACK PLASTIC columns, 12ml PLASTIC XL columns, 35ml PLASTIC MINI columns, 25pk PLASTIC MINI columns, 100pk EMPTY FPLC 8ml Columns, 3pk EMPTY FPLC 30ml Columns, 2pk EMPTY FPLC 45ml Columns, 2pk EMPTY FPLC 80ml Columns, 1pk Mag Rack (1.5 ml x 8 well)

Type Cat. No. Product Name

COLUMN PD-10,EMPTY

Microspin columns Microspin columns GE 對應商品

Crystallization

Screening Kit

蛋白質結晶條件多如繁星，

JBScreen

系列商品幫助實驗者快速篩選出最適宜的結晶條件！

提供 240 種最常見的緩衝液與沉澱劑之組合 整理出學者研究最容易成功的 96 種條件, 廣泛的 pH 範圍、多種沉澱劑 由 JCSG 機構發表，以稀疏矩陣為原理的篩選組合 針對 Protein Kinase 的初期結晶所設計，涵蓋了 96 種常見結晶條件 對於 PEG 3350 與 PEG 5000 MME 與 48 種鹽類組合的條件篩選

JBScreen Classic

JBScreen PEG/Salt

JBScreen Basic

JBScreen JCSG++

JBScreen Kinase

請前往下列網址瀏覽完整產品項目，或使用 QR code 以獲得進一步資訊

www.jenabioscience.com/cms/en/1/browse/632_crystal_screens.html

™

Crystallography Starter Kit

Detergents

l

包含一系列實用工具，讓使用者以省時省力的方式獲取結晶

l

MicroMounts™

具獨家光圈設計，減少取結晶時多餘液體

l

相容於常見

X

光設備，並具極低

X

光背景值

l

堅固耐用，即使在低溫條件下也不變形

JB-CSK-2 Crystallography Starter Kit, with everything to mount and collect X-ray data from your crystals Cat. No. Product Name

Each kit contains 20 Dual-Thickness MicroMounts™ (M2-L18SP-A2), with 5 each of 75, 100, 150 and 200 µm apertures. CU-16003 CU-16007 CU-16011 CU-16015 CU-16019 CU-16043 CU-16023 CU-16031 CU-16039 CU-16092 CU-16093 Octylglucopyranoside (OG) (5x5 g)

N,N-Dimethyl-n-dodecylamine N-oxide (LDAO) (5x5 g) n-Decyl-beta-maltoside (DM) (5x5 g) n-Dodecyl-beta-maltoside (DDM) (5x5 g) n-Octyl-1-thio-beta-D-glucopyranoside (OTG) (5x5 g) n-Decylphosphocholine (Fos-Choline™ 10) (5x5 g) n-Dodecyl-phosphocholine (Fos-Choline™ 12) (5x5 g) n-Tetradecylphosphocholine (Fos-Choline™ 14) (5x5 g) n-Hexadecylphosphocholine (Fos-Choline™ 16) (5x5 g)

Detergent Screening Set Classic (1 g each of OG, LDAO, DM, DDM, OTG)

Detergent Screening Set Phosphocholines 1 (1 g each of Fos-12, Fos-14, and Fos-16)

Features Cat. No. Product Name

純度 >

99.5%

專供結晶學使用

FluoroSpin

Protein Labeling and Purification Kit

FluoroSpin

能將廣泛的螢光物質與大於

25 kDa

的抗體、酵素與其它蛋白質快速地接合與純化。

其特點如下：

l

快速 – 標定加上純化，

10

分鐘就完成

l

簡單 – 只要三步驟：

Mix, Incubate, Purify

l

精確 – 可精確控制蛋白質標定的程度

l

方便 – 產品內已包含

Spin Columns

市⾯上最快速的ProteinLabelingKit!