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Nitric oxide induces heat shock protein 70 expression in vascular smooth muscle cells via activation of heat shock factor 1

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Figure

Figure 1. Western blot analysis of hsp70 protein in SMCs treated with NO donors. SMCs were dissociated from rat aortas by collage-nase, and were cultivated in 199 medium
Figure 4. Specificity of HSE-binding activity in SMCs in response to SNP. Nuclear proteins from SMCs treated with SNP at 37�C for 3 h were incubated with 32P-labeled HSE oligonucleotide in the presence or absence of unlabeled oligonucleotide containing HSE
Figure 6. Western analysis of HSF1 protein in control and NO-treated cells. Nuclear proteins prepared from SMCs treated with heat shock (42�C for 30 min), SNP, or S/NO for the indicated time were analyzed by Western immunoblotting for HSF1 protein
Figure 7. Effects of inhibitors on HSE-binding activity in nuclear proteins of SMCs. (presence (A) SMCs were treated with EGTA or hemoglobin in the �) or absence (�) of SNP at 37�C for 3 h, washed, and harvested with the cold buffer
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