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Genetic toxicity assessment of engineered nanoparticles using a 3D in vitro skin model (EpiDerm™)

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Figure

Fig. 1 BASF Levasil® silicon dioxide nanoparticle primary characterisation: Bright field TEM micrographs of (a) 16 nm-SiO2, and (b) 85 nm-SiO2,allowed primary particle size, shape and morphology to be assessed
Table 1 Levasil® nanoparticle primary characterisation: TEM in conjunction with EDX spectroscopy was used to determine primary size distributions, shape / morphology,crystallinity, composition and purity
Fig. 2 The reconstructed skin micronucleus assay optimised for nanomaterial test articles: (a) Six-well plate containing MatTek Corporation’s 3Depidermis (EpiDermTM) tissue models in trans-well inserts
Table 2 2D/3D dose normalisation and alternative metrics: Dose between 2D and 3D in vitro test systems was standardised inexposures (3D Topical) are presented in total mass (terms of total nanoparticle mass to total cell number at time of dosing (Table Lef
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