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Real-Time Video Imaging of Protease Expression In Vivo

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Figure

Figure 1. (a) Synthesis of the MMP-Pns. Reagents: a) HSPyU, 2% DIPEA/DMF, NH2-R1; b) 20% piperdine/DMF; c) Cy5.5-NHS ester, 2% DIPEA/DMF; d) TFA/water/TIS (95/2.5/2.5, v/v/v); e) BHQ-3 NHS ester, 2% DIPEA/DMF
Figure 2. (a) Recovery of fluorescence of the MMP-P0 (15 nM) in the presence of various activated MMPs (40 nmol/L) following 80 min incubation at 37 C
Figure 3. Whole-mouse imaging with Maestro 2 (CRI). (a) Representative serial fluorescence images of MMPs-positive SCC7 tumor-bearing mice injected intravenously with the MMP-Ps and MMPSense 680™
Figure 4. Representative serial fluorescence images of MMP-positive SCC7 tumor bearing mice injected intravenously with 150 L of MMPSense 680™ (2 nmol)
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