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Regeneration of TiO2 Nanotube Arrays after Long-Term Cell and Tissue Culture for Multiple Use – an Environmental Scanning Electron Microscopy (ESEM) Survey of Adult Pig Retina and beyond

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Figure

Fig. 1 ESEM images of L929 mouse fibroblast cultured on top of customized nanotube arrays with a tube diameter of (32 ± 3) nm for differentcultivation times: 1 day (a), 4 days (b) and 7 days (c)
Fig. 2 Structure of a TiO2 nanotube array with about 10 μm TiO2 nanotubes at the top and the bottom of the Ti-foil (left) and a schematic imageof the tissue culture setup (right), where a retina explant (black) lies on top of nanotube array (grey) which is
Fig. 3 ESEM images of PFA/GA-fixed retinal tissue residues on top of nanotube arrays after 14 days in culture: (a) overall view for retinal residues(b) microvilli from the RPE and (c) out-migrating cells which collect at the edges of the scaffold
Fig. 5 ESEM image of O30 min (2-plasma treated cell residues after 5 min (a) and 30 min (b) and an EDX-analysis of remaining filament-like residues afterc) which shows that the debris are made of phosphorous (P) and oxygen (O) laying directly on top of the TiO2-nanotube surface (Ti + O)
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