• No results found

One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system

N/A
N/A
Protected

Academic year: 2020

Share "One-step genetic correction of hemoglobin E/beta-thalassemia patient-derived iPSCs by the CRISPR/Cas9 system"

Copied!
11
0
0

Loading.... (view fulltext now)

Full text

Loading

Figure

Fig. 1 Strategy to target HbE mutation in Eoligo donor (ssODN) template to repair the mutation.β-iPSCs using CRISPR/Cas9 to induce a double-stranded break at the HBB locus and a single-stranded a Multiplex PCR analysis for HbE mutation
Fig. 2 Genetic correction of HbE mutation of thegenerated by gRNA1.spanning the gRNA target site in HbE-negative clones with HDR in the corrected clones and indels in others
Fig. 3 Hematopoietic differentiation of iPSCs using the feeder-free system.BMP-4 bone morphogenetic protein 4, VEGF vascular endothelial growth factor, KOSR knockout serum replacement, bFGF basic fibroblast growthfactor, BSA bovine serum albumin, SCF stem
Fig. 4 Hematopoietic differentiation of iPSCs using the OP9 coculture system and erythroid liquid culture.pared to peripheral blood CD34OP9 coculture (scale bar = 500transcription factors at day 19 of differentiation (equivalent to day 13 of erythroid liqu

References

Related documents

The depressed patient cohort consists of all patients over 18 years of age who meet criteria (explained below) ensuring that they: (i) indeed have depression, (ii) have a

National Conference on Technical Vocational Education, Training and Skills Development: A Roadmap for Empowerment (Dec. 2008): Ministry of Human Resource Development, Department

In earlier days classical inventory model like Harris [1] assumes that the depletion of inventory is due to a constant demand rate. But subsequently, it was noticed that depletion

Briefly, the other documents convey similar purposes: the Northern Territory‟s (2006) vision is that “all students make a successful transition from school to work, training

A significant decline in the concentrations of renal reduced glutathione in comparison with the control group (C) were observed in the trained and untrained rats intoxicated with

The LSA revealed that WAEE and CAEE have different effects on the life span of male and female adult flies in the long-term supplementation of extracts. WAEE has neutral

19% serve a county. Fourteen per cent of the centers provide service for adjoining states in addition to the states in which they are located; usually these adjoining states have

The example shows both, separate mathematical expressions (equation environment) and mathematics embedded in the text (enclosed by dollar-signs).. There is no difference in the