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Stereo-selective oxidations of terpinolene by cytochrome P450 monooxygenases in the microsomal fraction of Cupressus lusitanica cultured cells

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Figure

Fig. 1 Results of the chiral GC/MS of chemical synthesized IMEsmass spectrum of the peak at Rtmass spectrum of the peak at Rtthe peak at retention time (Rt)and enzymatic produced IME
Fig. 2 Determination of absolute configuration of the IME hydroxylSome protons inMosher’s method [group that appeared at Rt = 40.8 min in Fig
Fig. 3 GC/MS analysis of EMO produced by the microsomalspectrum of standard EMO.Sreaction with enantio-pure IME
Fig. 4 Chiral GC/MS analysis of EMO produced by the microsomalsimilar mass spectrum as EMO, indicating production of a singlemicrosomal cytochrome P450 frompeak than RtLower linepurity and configuration had been confirmed by Matsunaga et al

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