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xCELLigence system for real-time label-free monitoring of growth and viability of cell lines from hematological malignancies

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Figure

Figure 1 Fibronectin pre-coating significantly increases Jurkat, K562, and KMH2 cell attachment to the bottom of the culture well.Notes: (A) Fibronectin induced a rapid B-ci increase (,2 hours) in Jurkat cells
Figure 2 increases in the number of cells seeded into the wells, correlated with increases in ci.Notes: (A) We seeded different numbers of Jurkat cells (40,000, 80,000, 120,000, and 240,000 cells/well) in the presence (F+) or absence (F–) of fibronectin
Figure 3 Real-time monitoring of Jurkat/L1236 growth/attachment (leukemia).Notes: We seeded 120,000 cells (Jurkat/L1236) in duplicate in the presence (F+)/absence (F–) of fibronectin
Figure 4 Drug cytotoxicity monitoring of Jurkat/L1236 cells in the presence/absence of Fibronectin: RTCA vs MTT assay.decreases of the B-ci correlated with the presence of a large number of apoptotic cells as observed by microscopy (data not shown)
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