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In vitro Evaluation of Natural Marine Sponge Collagen as a Scaffold for Bone Tissue Engineering

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Figure

Table 1. Sequences of primers used for Real-time PCR
Figure 1: (A) Photograph of sponge samples that were collected, scale bar = 5cm; (B) SEM micrographs of sponge skeletons at low magnification showing the regular fibre network and irregularly arranged fibres of the sponge at low magnification; (C) high mag
Figure 3: Confocal microscopy showing F-actin fluorescent staining of cells (red) and nucleus (purple) on sponge skeletons (green autofluorescense) after 14 days culture
Figure 5. Light micrographs showing alkaline phosphatase staining. (A) Sponge skeletons with-out cells; (B) Sponge-cell constructs after 7days in vitro culture; (C) Sponge-cell constructs after 14 days in vitro culture; (D) Sponge-cell constructs after 21
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