Activity and Account Report 2012

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Activity and Account

Report 2012

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Index

1. Executive Summary... 4

2. Corporate Bodies... 7

3. Affiliates ... 8

4. Objectives and Strategy... 10

5. Operating Resources ... 13

5.1. Human Resources ... 13

5.2. Financial resources ... 13

5.3. Material Resources ... 13

6. Research Activities and Experimental Development (R&D) ... 14

6.1. Anchor Projects ... 14

6.1.1. Climate Change ... 14

6.1.1.1 Impact of Climate Change on Viticulture in the Douro Region ... 14

6.1.1.2 Short-term strategies for climate change mitigation in viticulture (ClimeVINESafe) ... 15

6.1.1.3 Modelling the Evolution of RDD Quality Wine ... 16

6.1.1.4 Management of water and heat stress on the grapevine ... 17

6.1.2. Eligible areas of grapevine crop potential ... 19

6.1.3.Functional Biodiversity in Viticulture ... 19

6.1.3.1 Biodivine (Demonstration of functional biodiversity at landscape wine - LIFE +) ... 19

6.1.3.2 ECOVITIS – Maximising ecosystem services in viticulture ... 22

6.1.4. Assessing the oenological potential of grapes ... 25

6.1.5. Preservation of grapevine genetic variability ... 26

6.1.6. Sustained production in viticulture ... 26

6.1.7. Rationalisation of the hillside vineyard ... 27

6.1.7.1 Economic Observatory ... 27

6.1.8. Skills development - Training and Dissemination ... 27

6. 2. Complementary R&D Projects ... 29

6.2.1. Evaluating the effectiveness of treatments to combat Oidium in grapevines at early stages in the season ... 29

7. Communication and Dissemination ... 31

7.1. Production of informative support material ... 31

7.2. Knowledge acquisition ... 33

7.2.1. Event participation ... 33

7.2.2. Training ... 35

7.3. Knowledge Transfer ... 35

7.3.1 ADVID page on the Internet - www.advid.pt ... 35

7.3.2 Publicity events ... 35

“Touriga Franca” workshop. ... 36

“Strategies for Weed Control in Organic Viticulture" workshop ... 37

"Climate Change in the Production of Wine. Overview and Assessment of the Situation in the Douro Region” seminar 38 “Analysis of the Evolution of Vegetative Cycle - Bud Burst” workshop ... 40

Open Day at the University of Porto ... 40

Workshop “Wine Spoilage Yeast - Brettanomyces-Dekkera” ... 41

XIX Annual proof of vintage ... 41

Biodiversity Week ... 42

“Bird watching at Quinta das Carvalhas” Session ... 42

Workshop “Valuation of Ecosystem Services in Viticulture” ... 43

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Institutional Module: "Biodiversity - Added Value in Wine?" In Infowine.fórum2012 ... 44

"Sustainability of Wine and Grapevine Production - Wine and Olive Oil Tastings" conference ... 44

“2012 Forecast Vintage Production” conference ... 45

”The Importance of Tracking Grape Ripening” workshop ... 46

"The Biodivine Project" publicity ... 46

Open Day at the University of Porto ... 47

“Installation of Hedges in Viticulture Ecosystem” workshop ... 47

“2012 Wine Balance” workshop ... 48

7.3.3. Papers Presented and/or Published ... 49

7.3.4. 2012 ADVID Award ... 51

7.3.5. Activation of Complementary Projects with Insertion into the Cluster ... 52

7.3.6. Media ... 53

8. Support Service for Good Agricultural Practices ... 55

8.1. Services financed by shares ... 55

8.1.1. Technical Support for Integrated Vineyard Production ... 55

8.1.2. Support for application process preparation of agro-environmental measures ... 56

8.1.3 Grape moth control by mating disruption ... 57

8.1.4 Forecast potential vintage by pollen method ... 57

8.1.5 Evaluation of grape variety and rootstock behaviour ... 58

8.2. Invoiced services ... 58

8.2.1. Viticulture ... 58

8.2.1.1 Support for organic production ... 58

8.2.1.2 Evaluation of water potential for irrigation needs ... 58

8.2.2. Oenology... 59

8.2.2.1 Maturation control ... 59

8.2.2.2 Portal of tannins (ADVID/AWRI) ... 59

9. Administrative and accounting support ... 59

10. Institutional Relations ... 60

11. Glossary ... 62

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1.

EXECUTIVE SUMMARY

In 2012, we continued working on the Action Plan for the 2009-2012 period after recognising ADVID as the operator of the wine cluster from the Douro Region. We developed the anchor projects that had been identified during recognition of the cluster and developed communication and dissemination skills, strengthened networks, promoted the transfer of knowledge from the science and technology sector to wine companies and improved technical support to Members.

Of all our 2012 activities, the following stand out:

The seminar Climate Change in Wine Production: A Global Overview and Assessment of the Situation of the Douro Region at the Luso-American Foundation (Fundação Luso-Americana) in Lisbon, and at Porto Customhouse, on 12 and 13 April.

In this seminar, findings from a study on the impact of climate change on viticulture in the RDD (Região Demarcada do Douro) were presented by Prof. Gregory Jones of the Department of Environmental Studies at Southern Oregon University (USA) in collaboration with ADVID. In addition, the effect of climate change in Portugal and Europe were presented by Prof. Pedro Miranda (Nova de Lisboa University) and Prof. João Santos (Trás-os-Montes and Alto Douro University), as well as the company strategy of Sogrape Vinhos SA, which was presented by Mr. George Sandeman, and Symington Family Estates, as represented by the engineer Charles Symington.

The Grapevine and Wine Institute, the Douro and Porto Wine Institute, ViniPortugal and the Luso-American Foundation joined up to support this event, in addition to the Minister of Agriculture, Sea, Environment and Spatial Planning, Prof. Assunção Cristas, who chaired the seminar opening in Lisbon.

Biodiversity Week (28-31 May) included the participation of Prof. Steve Wratten (Bio-Protection Research Centre of New Zealand), Dr. Howard Thistlewood (Pacific Agri-Food Research Centre of Canada), Prof. Laura Torres and Prof. Guilhermina Marques (UTAD) and the companies Real Companhia Velha, Sogevinus Fine Wines and Duorum Vinhos, represented by Mr. Rui Soares, Mr. José Manso and Mr. José Maria Soares Franco, respectively. In addition, the "Associação Portuguesa para a Diversidade da Videira" (Portuguese Association for Grapevine Diversity) participated, as represented by Dr. António Graça. Several activities were developed as follows:

 Bird watching session at Quinta das Carvalhas, Pinhão

 "Valuation of Ecosystem Services in Viticulture" workshop, Quinta do Tedo, Tabuaço.

 Birds and their role in biodiversity. Example of "Black Wheatear" (Bird of the Port Wine), Quinta de Castelo Melhor, Castelo Melhor.

 "Biodiversity - Added Value in Wine?" institutional module in Infowine.fórum2012, Vila Real.

UNESCO mission reception, as an interlocutor entity, by invitation of the Minister of Agriculture, Sea, Environment and Spatial Planning. The mission was to analyse the condition of the Douro World Heritage site and the impact of

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the Foz-Tua dam. This involvement culminated with an opinion about the "Impact of Hydroelectric Foz-Tua on Wine Production", as requested by the mission head for UNESCO.

Raising awareness in the Secretary of State for Rural Development on the issue of Flavescence dorée phytoplasma, which culminated with the drafting of the National Action Plan to Combat Flavescence Dorée Phytoplasma by the Working Group formed for this purpose, with which ADVID became integrated.

Meeting with the University of Porto in order to involve the scientific community of the largest Portuguese University in solving the winemaking problems of the Douro Region. This action resulted in a visit by 30 researchers from various colleges and research Centres from the UP in the Douro Region on 4 May and 8 October. Researchers covered issues relating to viticulture needs (mechanisation, erosion and landscape management) and oenological needs (maturity, harvest, grape-wine transfer quality, wine making and environmental management). As result, the first working theme was identified, namely, mechanisation.

The ADVID 2012 award to Mr. Cincinnato Costa for his work "General phenological model to characterise the timing of flowering and veraison of Vitis vinifera L." as developed by Amber K. Parker and C. Van Leeuwen - Enita, Université de Bordeaux and I. G. de Cortázar-Atauri from the I. Chuine Fonctionelle du Centre d'Ecologie et Evolutive in Montpellier. The ceremony took place at the General Assembly of 13 December 2013 at Quinta do Vallado and was attended by the honouree's granddaughter, Mrs. D. Maria Eduarda Costa Macedo, who presented the award to work coordinator Prof. van Leeuwen.

We highlight two factors relevant to the development of the Viticulture of the Douro Region program from 2012:

 The publication of the book "A Climate Assessment for the Douro Wine Region: An Examination for the Past, Present and Future Conditions for Wine Production", which resulted from work developed within the anchor project called "Climate Changes".

 Knowledge transmission to RDD grapevine growers by workshops/conferences (18), training/classes (6), informational brochures (15), technical books, technical notes (1), newsletters (10) and lists of plant protection products (7).

 Knowledge produced and disclosed in talks/seminars and technical-scientific papers (28);

 A new, more functional website that provides all available information To satisfy the members' needs, the following services were implemented:

• Measurements of grapevine water relations, by assessing predawn leaf water potential and the Sarmento hydraulic potential;

• Technical support for organic viticulture as presented by 3 technicians with appropriate training and a workshop on "strategies for weed control."

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the competitiveness of the wine sector and encourages entrepreneurship and innovation.

2.

CORPORATE BODIES

G

ENERAL ASSEMBLY

PRESIDENT: Adriano Ramos Pinto Vinhos S.A.

Represented by: João Rosas Nicolau de Almeida VICE-PRESIDENT: Niepoort (Vinhos), S.A.

Represented by: José Teles Dias da Silva MEMBER: Rozès, S.A.

Represented by: António Fernando da Cunha Saraiva

A

UDIT COMMITTEE

PRESIDENT: Cª Geral da Agricultura das Vinhas do Alto Douro Represented by: Pedro Silva Reis

MEMBER: Churchill Graham, Lda.

Represented by: John Graham MEMBER: Manuel Maria Gonçalves Mota

B

OARD

PRESIDENT: Sogevinus Fine Wines SA

Represented by: José Manuel Castro, Silva Meneres Manso

MEMBER: Sogrape Vinhos SA

Represented by: António da Rocha Graça

MEMBER: W & J Graham & CO SA

Represented by: Carlos Alberto Soares Caldeira

MEMBER: Quinta do Vallado - Sociedade Agrícola, Lda. (Agricultural Society) Represented by: Francisco Spratley Ferreira

MEMBER: Duorum Vinhos S.A*

Represented by: José Luís Moreira da Silva

* In a board meeting on 13 December, 2012, the inclusion of Associate Duorum Vinhos S.A. as a member of the Board, as represented by Dr. José Luís Moreira da Silva, was unanimously approved by cooptation among peers (based on paragraph 4 of Article #10 of the articles of association), to replace associate Lemos & Van Zeller.*

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3.

AFFILIATES

ACTIVE MEMBERS Quinta do Sairrão, Sociedade Agrícola, S.A. Quinta do Vallado - Sociedade Agrícola, Lda. Adriano Ramos Pinto - Vinhos, S.A. Quinta dos Avidagos, Lda.

C.ª Geral da Agricultura das Vinhas do Alto Douro Quinta Geração – Vinhos, Lda.

Churchill Graham, Lda. Quinta Nova de Nossa Senhora do Carmo, S.A. Niepoort (Vinhos), S.A. Rumo, Sociedade Agrícola, Lda.

Quinta do Noval - Vinhos, S.A. Sapec Agro, S.A.

Rozès, S.A. Saraiva & Filhos, Lda.

Sociedade Vinícola Terras de Valdigem, S.A. Silva & Cosens, Lda. Sogevinus Fine Wines, S.A. Sinergiae - Ambiente, Lda.

Sogrape Vinhos, S.A. Sociedade Agrícola da Quinta do Alvito, Lda.

W. & J. Graham, Ca. Sociedade Agrícola da Quinta do Vale de Malhadas, Lda. Sociedade Agrícola da Quinta do Vesúvio, Lda.

COLLECTIVE MEMBERS Sociedade Agrícola de Vila Velha, Lda. Sociedade Agrícola dos Canais, Lda.

A. Monteiro & Pôncio, Lda. Sociedade Agrícola José Mesquita Guimarães, Lda. Bayer CropScience Portugal, Lda. Sociedade Agrícola Quinta da Sequeira Nova, Lda. Beloxisto - Turismo Rural e Agricultura, Lda. Sociedade Agrícola Quinta da Teixeira Velha, Lda. Biosani - Agricultura Biológica e Protecção Integrada, Lda. Sociedade Agrícola Quinta do Beato, Lda. Cabanas - Sociedade Vitivinícola, Lda. Sociedade Agrícola Quinta do Todão, Lda. Casa Agrícola Horta Osório, S.A. Sociedade Agrícola Quinta Seara D'Ordens, Lda. Casa de Vilarelhos, Sociedade Agrícola, Lda. Sociedade Agroturistica da Casa dos Barros, Lda. Cockburn & Ca., S.A. Sogevinus Quintas, S.A.

Coimbra de Mattos, Lda. Symington Vinhos, S.A.

Duorum Vinhos, S.A. Syngenta Crop Protection, Lda. E.I. Empreendimentos e Investimentos Agrícolas do Douro, S.A. VCC, Unipessoal, Lda.

Esmero – Sociedade de Vinhos, Lda. Veredas do Douro - Sociedade Agrícola, Lda. F. Albuquerque e Filhos - Sociedade Agrícola, S.A. Warre & Companhia, SA.

F. Olazabal & Filhos, Lda. Wine & Soul, Lda. Fundação da Casa de Mateus

In Vino, Sociedade Agrícola, Lda. INDIVIDUALS

João Brito e Cunha, Lda.

João Nicolau de Almeida & Filhos, Lda. Afonso do Vale Coelho Pereira Cabral, Herdeiros

José Maria Pires – Qta. Vale de Locaia, Soc. Unipessoal, Lda. Alfredo Fernandes Teixeira Constantino, Cabeça de Casal da Herança de José Viseu Carvalho & Filhos, Lda. Álvaro Martinho Dias Lopes

Lemos & Van Zeller, Lda. Ana Maria Lencastre Sousa Soares Freitas Montez Champalimaud, Lda. Ana Paula Moreira Filipe de Castro Néctar da Sabedoria - Vinhos e Enoturismo, Lda. Ana Rita Forjaz Teixeira Leal Fráguas Pacheco & Irmãos, Lda. André Correia Cigarro Brás

Pagamo Meco, S.A. Antónia Maria Ponce Leão Bettencourt Mesquita de Araújo Prats & Symington, Lda. António Caetano Sousa Faria Girão

Quinta da Carvalhosa, Sociedade Agrícola, Lda. António Carlos Sobral Pinto Ribeiro Quinta da Jusã - Soc. Imobiliária e Turística, S.A. António da Cunha Carvalho

Quinta da Rosa - Vinhos, S.A. António dos Santos Cigarro, Cabeça de Casal da Herança de Quinta da Foz do Pinhão - Sociedade Agrícola, Lda. António José Silva Fonseca Gonçalves Mendes

Quinta do Grifo - Sociedade Agrícola, S.A. António Manuel da Costa Lima Acciaiuoli Dória Quinta da Xandica - Sociedade Unipessoal, Lda. António Manuel Rodrigues de Queiróz Quinta das Apegadas, Sociedade Agrícola, Lda. António Manuel Vicente Almeida Quinta de D. Matilde - Vinhos, Lda. António Manuel Vilhena Andrêz Quinta do Crasto, S.A. António Rodrigues de Carvalho

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Quinta do Pessegueiro - Sociedade Agrícola e Comercial, Lda. Bernardo Maria Freire Albuquerque Nápoles de Carvalho Quinta do Querindelo, Lda. Bertilde Botelho Elias

INDIVIDUALS (continuation) Maria Luísa da Graça Paulo Ferreira da Rocha Maria Manuela Matos Silva Fonseca

Carlos Alberto Sousa Sampaio Magalhães Maria Manuela Pizarro Montenegro Seixas Fego César Augusto Correia de Sequeira Maria Manuela Vasques Osório de Amorim Charles Andrew Nunes Symington Maria Natália Lameirão Monteiro de González

Domingos Guilhermino dos Reis Alves de Sousa Maria Paula Carmona de Abreu de Azeredo Malheiro Girão Eduardo Francisco Bessa da Costa Seixas Maria Virgínia Borges Gonçalves Costa Mendes Eunice Luís Silva Fonseca Gonçalves Mendes Mário Joaquim da Rocha Braga, Herdeiros Fernando de Sousa Botelho Albuquerque Natália Neusa Correia Cigarro Miranda Brás Francisco Júlio Marinho Oliveira Passos Paul Douglas Symington

Inês Sofia Gomes de Sousa Botelho Albuquerque Pedro Pinto da Cunha Rola Isabel Maria da Costa Fevereiro Peter Ronald Symington João Baptista de Castro Girão de Azeredo Leme Pompeu Barros Viseu João Henrique Pereira da Silva e Sousa Pessanha Martins Moreira Pôncio Martins Ribeiro

João Manuel Araújo dos Santos Tomás Guedes de Almeida Holtreman Roquette

João Pereira Rebelo Vicent Bouchard

Joaquim Norberto Campos Rodrigues dos Santos

John Andrew Douglas Symington HONORARY MEMBERS

Jorge Bernardo Lacerda de Queirós

Jorge Botelho Elias Acácio Manuel Poças Maia

Jorge Rosas Vinhos Unipessoal, Lda. Alexandre José Pina de Carvalho José Agostinho Fernandes Lacerda António Rocha Pinto

José Alberto Pinto de Azevedo António de Vasconcelos Maia

José António Teixeira Martins António J. Albuquerque de Oliveira Quinta José Arnaldo Coutinho António J.S. de Oliveira Bessa José Carlos de Morais Calheiros Cruz, Herdeiros António Jorge Ferreira Filipe José Correia Barrigas de Azevedo Charles Andrew Nunes Symington José Luís Matos Rodrigues de Figueiredo Christian Seely

José Manuel Morais Barata Cristiano José Seabra Van Zeller José Maria Ramos, Herdeiros Fernando Bianchi de Aguiar

José Ramos da Fonseca Fernando Luís Van Zeller

Laura Maria Osório de Meneses Braga Coutinho Albuquerque Nápoles Francisco Barata Tovar Laura Maria Valente Barreto Nogueira Regueiro George T.D. Sandeman Luís Baptista Pinto de Figueiredo, Cabeça de Casal João Manuel M. de Almeida Barros Macário de Castro da Fonseca Pereira Coutinho João Pedro Larangeiro Ramalho Manuel Cândido Pinto de Oliveira John Gordon Guimarães Manuel da Costa Pinto Hespanhol José Alfredo Pinto Gaspar

Manuel Fernandes Lebres José Manuel Froés Burguete de Sousa Soares Manuel Joaquim Freire D'Almeida Gouveia José Maria d’Orey Soares Franco

Manuel Mouzinho de Albuquerque de Mascarenhas Gaivão Manuel Ângelo Oliveira de Almeida Barros Maria Adelaide Pinto dos Santos Miguel Côrte-Real da Silva Gomes Maria Alcina Fortes de Carvalho Nuno d’Orey Cancela de Abreu Maria Amélia Branco Xavier de Araújo Nuno Pizarro Magalhães Maria Amélia Cyrne Correia Pacheco Lobato Faria Pedro Miguel Cunha de Sá Maria Antónia de Nazaret Bernardo Azevedo Narciso Peter Ronald Symington Maria Beirão Costa Pinto Almeida Silva

Maria Celina Gomes Parente do Patrocínio Maria da Graça Almeida Ferreira de Sousa Pizarro Maria da Graça Lacerda de Queirós

Maria de Fátima Pimentel Teixeira Correia Tavares de Figueiroa Rego Maria Emília Miranda de Sousa Leite Lobo D'Ávila

Maria Etelvina Ferreira Trigo Pereira Carneiro Maria Henriqueta Janeiro Pinto da Silva Maria Isabel Junqueiro Sarmento Gomes Mota

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4.

OBJECTIVES AND STRATEGY

The three-year-old wine cluster of the Douro Region has the mission of “streamlining and consolidating the wine production in the Douro region through a technological and sustainable strategy applied to their stakeholders”, whose vision points to an "environmental, economic and socially sustainable sector, excited by a culture of network cooperation to reduce threats and optimise opportunities".

To pursue the mission, Cluster Wines of RDD presents the following strategic objectives:

 Increase the number of associates and, consequently, the resources to develop and boost the Cluster;

 Promote and increase investment in R&D and company innovation through action projects to add value to grapevine-wine activities;

 Increase the level of technical and economical skills for the sector as a competitive advantage for the companies in Portugal and worldwide;

 Optimise, increase and transmit knowledge within the region as a way to improve viticultural practices, increasing the value of local human capital;

 Development of methods to favour the operating efficiency of grapevine-wine production;

 Raising public and private funding for the Region;

 Establish platforms of scientific knowledge and support initiatives for economic agents to innovate processes and products;

 Creation of services to support companies.

To meet these goals, a set of initiatives was defined and integrated into an action plan focusing on the innovation, qualification and modernisation of companies in the sector, which will encourage the emergence of agglomeration economies through cooperation and networking.

The action plan has a mobilising nature that potentially generates positive externalities for the cluster agents and the wine and grapevine goals include:

Anchor Projects and Strategic Lines of Development, with a high degree of partner participation, both from the consortia and groups with common interests;

Complementary Projects, intended for individual actions or companies in cooperation, for which incentive lines are available with positive outcomes for integrated actions within the cluster objectives;

Webcasts of meetings and workshops with cluster partners and counterparts.

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To monitor and evaluate the achievement of objectives, targets and indicators were defined, and the 2012 results are shown in the tables below.

Table 1 – Indicators and respective targets defined for 2012

Result indicators Unit Expected Achieved

Variation in the number of affiliates % 2 -3.9

Training actions and promotion events Number 50 125 Dissemination of technical information and publications

available to public domain Number 200 181

Hours spent on technical training Number 500 948

Technical services provided by the Association Number 10 7

Reference parcels on ADVID network Number 15 22

New production processes developed Number 8 7

Preservation of grape genetic variability varieties grape 50 50

Funded R&D performed Number 15 11

Projects with intervention of SCT entities Number 15 9 Increased spending on R&D by the cluster % 20 - Increased number of ADVID researchers and main partners Number 5 4 Participation in international R&D programs Number of

projects 3 4

Projects approved with public funding Number 14 11

Impact indicators Unit Expected Achieved

Environmental awareness actions Number 10 74

Increase RDD attractiveness – Number of visitors % 5 -

Increased RDD exports % 1 5.2

Change in income (increased turnover) % 1 3.8

Reduction of production costs % 5 -

Production units subject to modernisation Number 500 >500 Creating skilled jobs - Level IV or higher Number 10 4

Variation in workforce skill levels % 15 14

Number of companies that changed from SMEs to large

companies Number - -

Demonstration sessions and dissemination of results Number 50 125 Technical information disseminated and publications placed

in the public domain

Number

200 181 Projects with dissemination at national-international levels Number 6 11

For some indicators, the targets achieved at the end of three CEE Cluster Wines of the Douro Region years fell short compared to the forecasts, namely:

 Variation in the number of affiliates

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 New production processes developed

 Funded R&D performed

 Projects with SCT entity intervention

 Participation in international R&D programs

 Projects approved with public funding

 Creating skilled jobs - Level IV or higher

 Variation in workforce skill levels

With respect to the negative variation in the number of affiliates, the reason is essentially related to a reduction in the number of individual members. However, there was an increase in the value generated by quotas due to a significant increase in the number of collective members, as shown in graph I.

Graph 1 – Evolution of Affiliation Structure – 2012

With respect to other indicators, deviation occurred as a consequence of the current situation and lack of tender for public financing for the Strategic Lines of Development (anchor projects), which has implications for the various planned activities.

We choose not to consider the indicators “Increased spending on R&D in the Cluster”, “Increase the RDD

2002 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 Individual members 122 137 151 129 128 123 115 112 107 96 92 Collective members 40 46 51 47 51 56 55 60 63 69 69 Effective members 11 13 13 11 11 9 9 9 10 11 10 Fixed shares (€‘000) 106,44 123,84 128,52 111,48 113,76 104,16 102,60 105,24 107,46 118,34 113,22 Variable shares (€‘000) 202,77 219,18 237,76 234,23 206,61 174,57 140,14 108,02 118,43 124,41 116,97 Total shares (€‘000) 309,21 343,02 366,28 345,71 320,37 278,73 242,74 213,26 225,89 242,75 230,19 0 50 100 150 200 250 300 350 400

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also because of the current situation.

5.

OPERATING RESOURCES

5.1.

Human Resources

The ADVID team is made up of one General Director, one Technical Director, 6 technicians and one manager. Two IEFP trainees were hired in addition to one technician in order to support the projects, and one trainee was hired by the Maximising Ecosystem Services and Biodivine projects, respectively.

5.2.

Financial Resources

2011 2012 Effective members 11 10 Collective members 69 69 Individual members 96 92 Fixed shares ('000€) 110.00 11322 Variable shares ('000€) 112.74 116.97 Total shares ('000€) 222.74 230.19

5.3. Material Resources

Material Number Laptops 10 Desktops 3 Notebooks 1 GPS 1

Multifunction Printer / Fax 2

Colour Ink Jet Printer 1

Multifunction Copier 1 Multimedia Projector 1 Camera 3 Cars 5 Spectral Radiometer 1 Pressure chamber 4 Centrifuge 1 Titrator 1 Blender 2 Potentiometer 1 UV / Vis spectrophotometer 1 Glass washer 1 Benchtop refractometer 1

Pocket refractometer (portable) 1

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Material Number

Surveillance cameras 9

Soil moisture probes 2

RAC 1

Server 1

Data loggers 4

Climate control 5

Binocular magnifying lens 2

Magnifying lens 1

Stove 1

Microvinification vats 10

Minipress 1

6.

RESEARCH AND EXPERIMENTAL DEVELOPMENT ACTIVITIES (R&D)

6.1.

Anchor Projects

6.1.1.

Climate Changes

The development of this anchor project was based on three complementary actions: “Evaluation of the climate of the Douro Region – by analysing the past, present and future climate behaviour to produce wine” as well as by using the definitions of efficient adaptation and by forecasting strategy influences on wine quality. These actions, which were promoted and sponsored by the Douro Region Wine Cluster, have integrated various important players from the “ClimeVineSafe” projects, who had the task of developing short-term measures to mitigate the effects of climate change and support the “Evolution Modelling of Wine Quality in the RDD” project.

6.1.1.1 Impact of Climate Change on Viticulture in the Douro Region

To carry out this anchor project component dedicated to understanding the climate in the Douro Region, the ADVID collaborated with prestigious American scientist Professor. Dr. Gregory Jones (Southern Oregon University), an expert on the effects of climate change on viticulture. The work consisted in the analysis, stabilisation and credibility of existing climate databases and structuring new approaches to interpret the regional climate, contributing to a better understanding, which is an essential condition for projecting future scenarios. The main 2012 results of this collaboration were the following activities:

 Conclusion of tasks contracted in collaboration with Professor Gregory Jones (Southern Oregon University, Oregon USA), relative to the component "Climate Assessment in the Douro Region" as well as the support for complementary studies on atmospheric circulation effects in the development of grapevine and local production, support for the development and calibration of a reference index (benchmark) to the vintage quality, product development to improve the spatial resolution of climate data for the region and the

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development and calibration of a zoning model for local production variability and phenology mapping of the Douro Region.

 Project result publication in the book "A Climate Assessment for the Douro Wine Region: An Examination for the Past, Present and Future Conditions for Wine Production", ADVID, 98 pages.

 Presentation of the main results in the seminar "Alterações Climáticas na Produção de Vinho. Visão global e avaliação da situação na Região do Douro [Climate Change in Wine Production. Global Overview and assessment of the situation of the Douro Region]" at the Luso-American Foundation in Lisbon, and at Porto Customhouse, on 12 and 13 April.

 Publication of the work "Impacts of Climate Change on Wine Production: A Global Overview and Regional Assessment in the Douro Valley of Portugal" in the “International Journal on Global Warming (IJGW)”. Gregory V. Jones and Fernando Alves, Vol. 4, No. 3/4, 2012.

 Participation in the IX Congrés International Terroirs Vitivinicoles, on 25 and 29 June at Dijon (Burgundy) and Reims (Champagne), with analysis and characterisation of climate conditions on Portuguese vines presented in "Spatial analysis of climate in wine grape growing regions in Portugal".

 Compilation and structure of phenological information to develop phenological mapping of the main RDD grapes and climate influence on the definition of different vegetative cycle phases.

6.1.1.2 Short-term strategies for climate change mitigation in viticulture (ClimeVINE

Safe)

Preparation of field tests, choice of plots and procedures to deploy in order to investigate the behaviour both in pots and in commercial grapevine situations.

Launch and selection process for a FCT scholarship to support experimental trials and data collection for the project.

Conducting field tests in plots at Touriga Nacional and Touriga Franca, Quinta do Vallado.

Preparing scenarios for the RDD together with earlier simulations of climate change by using high-resolution Ensemble downscaling. As a result of this work, the following study was concluded: "Ensemble projections for wine production in the Douro Valley of Portugal". J. A. Santos & S. D. Grätsch & M. K. Karremann & G. V. Jones & J. G. Pinto. Journal “Climatic Change”. Online Publication on 13 July 2012.

In addition to project development, the following work was prepared and submitted for publication in the journal Agriculture, Ecosystems and Environment: "Very high resolution bioclimatic zoning of Portuguese wine regions: present and future scenarios" Fraga, H.; Malheiro, A. C.; Moutinho-Pereira, J.; Jones, G. V.; Alves, F.; Pinto, J. G. 4,5; Santos, J. A.

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6.1.1.3 Modelling the Evolution of RDD Wine Quality

Supporting benchmark development to assess the quality of the harvest was achieved with the participation of Professor Gregory Jones by following the collaboration agreement with ADVID.

Following the activity in question, the following work was prepared:

 Progress report of the PhD project (FEUP): "Modelação da qualidade do vinho na Região do Douro [Modelling the quality of the wine in the Douro Region]".

 “A new method to obtain a consensus ranking of a region’s vintages' quality”. José Borges, António C. Real, J. Sarsfield Cabral and Gregory V. Jones. Published in the Journal of Wine Economics, Volume 7, Issue 01, May 2012.

The XIX Prova Anual de Vinhos de Vindima (XIX Annual Vintage Wine Tasting) on 15, 18, 22 and 23 May 2012, at the Escola Superior de Biotecnologia (ESB) (School of Biotechnology), Porto. Sixty-six wines were tasted, 31 were "generous" and 35 fit in the Douro region.

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Fig. 1 -Distribution of the tasted samples, by type of wine and grape variety. (QM - Mean quality; QR - Representative Quality; TR – “Tinta Roriz”, TN – “Touriga Nacional”, TF – “Touriga Franca”, TA – “Tinta Amarela”, SO – “Sousão”, TC – “Tinto Cão”

6.1.1.4 Management of grapevine water and heat stress

This project aimed to explore the effects of different water regimes on the viticultural and oenological behaviour of the Touriga Nacional grape variety. This project started in 2012, and the Quinta dos Aciprestes, Quinta do Noval and Quintas da Canameira e Grifo were used as reference plots in the high Douro region.

With the extension of the spatial monitoring of grapevine water status for the high Douro region, we sought to assess the basis for services development for members, which started in 2012. Three farms have acceded to this process (see Point 8 - Services). In brief, the objectives and work performed were completed in the Quinta dos Aciprestes (Soutelo do Douro).

In this experiment, the vine water status had been monitored since 2002 and was subject to three drop-by-drop irrigation depths (0%, 30% and 60% of ETc - Corrected Potential Evapotranspiration). Predawn leaf water potential follow-up was made between June and the end of September.

We observed the maturation evolution through the analysis and summary of the phenolic components. The productive parameter behaviour of the different modalities was observed in grapevines.

This project included the work "Influence of climate and deficit irrigation on grapevine physiology, yield

and quality attributes of the cv Touriga Nacional at Douro Region" presented at the IX Congrés

International Terroirs Vitivinicoles (Dijon/Reims, France).

Fig. 2 – Evolution of the predawn leaf water potential values (2002-2012) in the reference plot at Soutelo do Douro (T. Nacional x 196-17), values of non-watered repetitions (n = 24)

-1,4 -1,2 -1,0 -0,8 -0,6 -0,4 -0,2 0,0 15/6 22/6 29/6 6/7 13/7 20/7 27/7 3/8 10/8 17/8 24/8 31/8 7/9 14/9 21/9 2002 2003 2004 2005 2006 2007 2008 Pred awn leaf water po tent ial Ѱp d (MPa ) 2009 2010 2011 2012

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Quinta do Noval (Pinhão)

In this test plot, which was installed in a vineyard organised into micro levels in rows, two modes (0% and 30% of the ETc) with three replications were investigated. The irrigation amount was lower than those of the other test sites because there was a smaller need for watering as identified by monitoring and the observation of predawn leaf water potential. We evaluated productive and qualitative parameters, and prepared a report on the results of the study at Quinta do Noval.

Quinta da Canameira / Grifo (Barca d´Alva)

To investigate the response of the Touriga Nacional and Touriga Franca varieties in an environment with more water-stress, new observation plots were defined in 2011 in the high Douro for monitoring the predawn leaf water potential, maturation and production. In 2012, we continued observing the evolution of predawn water potential and the response of productive and qualitative parameters in terms of water stress in a study predefined by ETPc and monitored by the predawn water potential. Result reports were made and discussed with the Rozès Vinhos SA.

6.1.2.

Eligible areas of grapevine crop potential

The preparation and development of contacts and meetings with partners and companies with expertise in the area of remote sensing were carried out to support zoning processes and precision viticulture.

The mapping of spatial analysis for physiographic parameters of the region were developed to classify the plots. Preparatory work for the implementation of a web mapping platform (transfer of information) was done to support the quality potential management of the vineyards.

6.1.3.

Functional Biodiversity in Viticulture

Increasing functional biodiversity in vineyards (value creation) was accomplished, which valued the establishment of ecological corridors and reduced pesticide inputs. The anchor project "Functional Biodiversity in Viticulture" is supported by three projects sponsored by different financing sources, namely "The green infrastructure in conservation biological protection against pests in the vineyard - IEE", "ECOVITIS - Maximising ecosystem services in the grapevine business," and "BioDiVine - Demonstration of functional biodiversity in the landscape". We will summarise the main 2012 achievements. These actions converge to one main goal as follows: to promote the maintenance of productive agricultural system sustainability and create value through ecosystem services. Today, the actions expected for the IEE project are part of the ECOVITIS project.

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6.1.3.1 BIODIVINE (Demonstration of functional biodiversity in landscape wine - LIFE

+)

Monitoring Actions

Monitoring arthropods

The sampling location was changed; during the first year, it was located in five distinct habitats, and in the second year, it was a grapevine habitat assessment according to the composition of the landscape. For this study, the 25 sampling points were located at a distance of 1 km to permit the analysis of a buffer per sampling point of 500 m, for a total of 2000 hectares for an analysis of the landscape composition associated with the sampling point.

Similar to the experiments from 2011, arthropods were monitored by using combination traps (to catch flying arthropods) and pitfall traps (for capturing terrestrial arthropods) from 25 plots located in the Cima Corgo sub region (RDD). The captured material was classified by RBA method, recorded, photographed (to create a photo library) and saved. This work was performed by the Master's student Nathan Pigeon and resulted in the preparation of his thesis, which was disclosed to members.

Monitoring flora

Organisation of the flora monitoring protocol and training of the Master's student, who was responsible for surveys intended for all European partners.

In each of the 25 sites, florasurveys were conducted during the month of March by a Master's student who was hired for the project and developed a thesis called "Characterisation of the flora from six vineyards of Europe" on the basis of a vegetation cover comparison observed at six project demonstration sites, which includes the Douro. This work was performed by the MSc student Miren Pédehontaa, resulting in the preparation of her thesis, which was also disclosed to members.

Monitoring bird life

During the month of October, bird monitoring was conducted in each of the 25 plots under observation by the Laboratory of Applied Ecology team of UTAD, which was hired by ADVID for this purpose. This monitoring included visual observations and listening to the birds present in the vineyard ecosystem for approximately 10 minutes per site. Forty species of birds were observed in the vineyards, among which we highlight the following in order of decreasing abundance: the European robin, the blackbird, the warbler, the European serin, the great tit, the starling, the redstart and finches.

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Ground cover plays an important role in soil protection (e.g., through the reduction of erosion, promotion of organic matter and filtration of water resources), and it provides other ecosystem services such as the biological control of pests and diseases, soil aeration and soil nutrient recycling. To monitor the biological activity of soil (presence of invertebrates), two portions were delimited at the top, one with and one without cover crops located in proximity to one another at Quinta do Seixo. For the monitoring, we used the easy "bait-blades" tool, which was made from a plastic stick with 16 holes containing cellulose powder plus bran and activated charcoal flakes, which, when eaten by soil invertebrates, provided an indication of biological activity in the soil. Within a given time interval, the more holes that were empty, the more “bio-active” the soil was. The soil analysis (routine and microbial biomass) has not yet been completed for result interpretation.

Monitoring mammals

The BioDivine project aims to monitor mammals in the grapevine ecosystem by using infrared (IR) cameras located along the border area in three habitats of the study (1 - riparian gallery, 2 – woods, 3 - olive/vineyards with cover crops). The impact of the presence of such infrastructure in the vineyard proximity was evaluated. At the end of the first 12 weeks of monitoring, between December 2012 and February 2013, a brief assessment of collected material was done with short films recorded when mammals crossed the area by using IR cameras.

In descending order of frequency records, we highlight the presence of the following mammals: 1st rabbit (164 records), 2nd fox (68 records), 3rd boar (29 records), 4th mouse (20 records), 5th ferret (6 records) and 6th hedgehog (4 records).

We found that all three habitats located in the monitored vineyards captured a large number of videos. The highest frequency of records was observed in one of the olive tree orchards with ground cover, most likely because this orchard is connected to a large wooded area.

Implementation of conservation actions

Demonstration actions were prepared (in relation to ground cover, redefinition of headlands, hedge installation and mating disruption for grape moths).

In December 2012, ADVID launched a bid for its members to take advantage of funding (50%) to implement 5 conservation actions for the promotion of ecosystem services: C1-ground cover, C2-installation of hedges, C3-promoting biodiversity, C4-use of non-chemical control methods (pheromones for mating disruption), C5-reorganisation of headlands. Three of these five actions were adopted to a greater extent by members, namely the C1-ground cover (expected 12.5 hectares of green cover), C2-installation of hedges (planned to install 2600 metres

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Dissemination of actions

 Development of the project webpage available at www.biodivine.eu

 At the Conference OILB "Landscape management for functional biodiversity" held on 7 and 10 May 2012 in Lleida (Spain), the paper “Monitoring arthropod diversity in Douro wine region vineyards” was presented.

 Measures to disseminate the preliminary results on 3 September and 25 October for ADVID members.

 Workshop "Instalação de sebes no ecossistema vitícola" (Installation of hedgerows in the grapevine ecosystem), 29 October, Pinhão.

 Management and monitoring meeting in Villafranca de Penedes (Catalonia - Spain) on 10 and 11 May.

 Follow-up and project management meeting in Limoux (France) on 17 and 18 October.

6.1.3.2 EcoVitis - Maximising ecosystem services in viticulture

Monitoring the impact of ecological infrastructures with chromotropic traps

To assess the impact of woods on the presence of arthropods in the vineyard and thereby explain its importance in the infrastructure of the project, we placed yellow sticky traps in two plots of Quinta das Carvalhas and in one plot of Quinta de Arnozelo in early May. In the Quinta das Carvalhas plots, there were three modes, each of which had four replicates (i.e., traps), according to how distant they were from the woods: 0, 25 m and 50 m. In the Quinta do Arnozelo, the same distances were used although only one trap was set at 0 m as a consequence of logistical issues. Three traps were set at each of the other two distances. The traps were observed weekly for a total of 24 weeks to record the captured arthropods. These arthropods totalled 13,286 individuals, which were distributed along the different distances/replicates that were screened to identify their order or, when possible, their family. We also separated them by trophic groups, i.e., parasitoids, predators, phytophagous and unknown, as we proceeded with the material sampled in 2011.

Impact assessment of ecological infrastructure in the presence of grape moth parasitoids

At two different time intervals (May-June and September-October), which represents the 1st and 3rd generations of the grape moth, and in various locations of the farms that comprise the project, caterpillars were collected to be grown under lab conditions in order to obtain parasitoids and evaluate their importance. Collection was not made during the 2nd generation because of their low incidence during that year of analysis. In total, 743 moth specimens were collected, of which 41.7% were parasitised. A total of 91.4% of the parasitoids were Elachertus affinis. The remaining specimens were sent to the Natural History Museum of Paris to be identified by entomologist Dr. Claire

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Villemant. The UTAD researcher responsible for flora, António Crespí, together with the ADVID team, made floristic inventories of the plant communities analysed in our activities.

Prospecting parasitoids of grapevine mealybugs

In the parasitoid evaluation, observations were made on the complex associated with grapevine mealybugs (life cycle, natural enemies and evaluation of the impact of the landscape on pest occurrence and action of natural limiting factors). These observations were made for the September/October vintage by the UTAD team together with the ADVID team. The number of grape clusters attacked by either coccidian or pseudo-coccidian mealy bugs was recorded. A total of 68 plots were observed, of which 57.4% were attacked by mealybugs (44.1% pseudo-coccidian and 25% pseudo-coccidian). The projects were restarted in early December in order to analyse the population dynamics of mealybugs during the winter. To obtain in depth information, observations were made at two project sites and were extended to other two plots that were not previously included in the project because there was a greater incidence observed there.

Application of grape moth mating disruption in the Douro region

Pheromones for mating disruption were applied with the same resources used in 2011 (ISONET - LTT, each containing 300 mg (E, Z)-7,9-dodecadienyl acetate - sex pheromone from the grape moth Lobesia botrana), in the entirety of those 5 sites planned for the project. The applied 173 ha area was larger than that from 2011. It was not possible to make observations because the action a) of phase 1 of the project was not concluded. Diffusers based on the use of GIS had not yet been developed. The results were described in a preliminary report made by technicians at the sites following method efficiency evaluation.

Evaluating the presence of other grape moths (Ephestia sp.)

Over the course of the project's development, we observed the occurrence, in significant numbers, of "other moths" (i.e., larvae from other Lepidopteran species causing damage similar to that generated by the species commonly called grape moth), in addition to L. botrana, at Quintas das Carvalhas, S. Luiz and Arnozelo. In response to the importance of these insects (mostly individuals of the genus Ephestia) and the possibility of their interference in the use of mating disruption to combat L. botrana, we decided to devote attention to these pests within this project. Therefore, we contacted Italian researchers who had experienced this identical situation in Italy. From this contact, samples of collected material were sent to Dr. Andrea Lucchi Coltivazione and Difesa delle Specie Legnose at the Department of Rural Entomology in Pisa, Italy for identification. In parallel, we sought to explain the occurrence of such insects, which allowed us to establish positive correlations between their populations and the dehydration of clusters, the occurrence of blossom drop and Glomerella cingulata (Stonemam) attack. These

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to combat L. Botrana.

Isolation and analysis of entomopathogenic fungi and their genetic diversity as associated with the cultivation of vineyards in the Douro Region

The use of entomopathogenic fungi is an important tool in pest biological control, whether in conventional agriculture, organic farming or integrated production. The development of methodologies to identify or unequivocally characterise the species of fungi found in the Douro region is therefore one of the priorities of this area of research. The methodology, which employed Tenebrio molitor larvae as a trap, was adequate for the isolation of entomopathogenic fungi from the soil. Studies conducted in 2012 showed the occurrence of entomopathogenic fungi from the genera Beauveria, Metarhizium and Lecanicillium. There was also the emergence of a new species, located in different parts of the vineyard, which is undergoing sequencing and molecular identification. The genetic work in progress will optimise the biotic potential of natural entomopathogenic fungi populations to combat pests.

Dissemination actions

"Biodiversity Week" was promoted in May, with several activities and the presence of Professor Steve Wratten (Lincoln University):

"Valuing Ecosystem Services in Viticulture Activity"

Professor Steve Wratten (Lincoln University), one of the leading experts in functional biodiversity in the viticulture field, attended this workshop to share his experience in this area. The following communications were presented:

o "Conservation Biological Protection in the Alto Douro vineyards. Associated ecosystem services" (Laura Torres, CITAB-UTAD)

o "Functional biodiversity of the soil: What is the role of entomopathogenic fungi?" (Guilhermina Marques, UTAD)

o "Biodiversity of plants and arthropods associated with RDD vintages " (Cristina Carlos, ADVID)

o "Biodiversity in viticulture - Essays in Sogevinus and Real Companhia Velha" (José Manso, SOGEVINUS, Rui Soares, REAL COMPANHIA VELHA)

o "Conservation of Genetic Resources of the Grapevine in Portugal. A challenge to the sustainability of the wine sector" (António Graça, SOGRAPE)

o "Biodiversity in vineyards - Worth the bother", (Steve Wratten, Lincoln University, NZ) o Poster presentation "Flora Arthropods of the Douro Region" (Cristina Carlos et al.)

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“Biodiversity - Value added in Wine?” (Infowine forum 2012)

For the Infowine.forum 2012, the Institutional Module "Biodiversity - Value added in Wine?" was organised under the following program:

o Biodiversity and ecosystem services in the landscape of the Alto Douro wine region. Laura Torres (CITAB- UTAD).

o Functional biodiversity in vineyards of the Douro Region - environmental benefits and value creation.

Cristina Carlos (ADVID).

o Conservation of genetic resources of the grapevine in Portugal. A challenge to the sustainability of the wine sector. António Graça (Sogrape Vinhos S.A.).

o Green-valued products (green) in the external market. Nuno Vilela (Sinergiae Ambiente) and José Maria Soares Franco (Duorum Vinhos S.A.).

o Functional biodiversity in the vineyard of the Douro region, management and promotion. Personal experience of sogevinus fine wines, S.A. Mr. José Manso (Sogevinus, Quintas S.A).

o Functional biodiversity in the vineyards of the Douro region, management and promotion. Personal experience of Real Companhia Velha. Mr. Rui Soares (Real Companhia Velha).

“Bird watching at Quinta das Carvalhas” Session

Birds and their role in biodiversity. Example of the Black Wheatear (Bird Port) – Bird watching session.

The following works were also presented:

"Biodiversity of plants and arthropods in key ecological structures of vineyards of the Alto Douro

region", at the OILB Conference “Landscape management for functional biodiversity” on 7 and 10 May

2012, at Lleida (Spain).

 "Report biodiversity/sustainability and added value" in the workshop “Viticulture and biodiversity, good agricultural practices and value added”. State Agriculture Department, the Palace Square, Lisbon, 20 November.

"The importance of diversity in the perspective of the industry". Seminar: "Grapevine diversity, sustainability factor of the wine sector and of the territory" held in Palmela, 22 November.

 "Maximising Grapevine Ecosystem Services in the Douro Region". Day of innovation in UTAD. UTAD, Vila Real, 28 November.

"Biodiversity in landscape management and supporting plant protection (functional)". VINIPORTUGAL annual forum with the theme "Sustainability applied to wine." Palácio da Bolsa, Porto, 28 November.

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6.1.4 Assessing the oenological potential of grapes

We held meetings and made contact with members and partner organisations of the Cluster to carry out the following:

 Redefine the specifications for project development;

 List activities to develop, elect representative parameters and choose the application process and technology to implement.

 Developing contacts with organisations and companies that hold prototypes and equipment in the validation phase;

 Submission of grant application to SAESCTN in co-promotion with the University of Porto (School of Pharmacy - laboratory-associated REQUIMTE) and College of Engineering, for the project "iGrape: definition and establishment of quality indices for wine grapes”. This project, which is a collaboration between ICETA, ADVID and INESC, aims to assess the current situation in terms of producer grape valuation and developing a strategy to integrate the available information by some indices that can be used in the future as credible indicators of grape quality, and to implement one analytical technique for in-situ maturation measurement of grapes in the vineyard during the ripening period (infrared spectroscopy);

 Validation of methodologies (already initiated);

 Developing contacts with organisations and companies holding prototypes and equipment in the validation phase.

6.1.5 Preservation of grape vine genetic variability

In continuing the 2011 projects, we kept exploring and harvesting plant material from 23 vineyards with 74 different grape varieties and 395 plants, for a total of 25 plots/winemakers.

In line with this 2012 activity (diversity preservation and study of plant material characteristics), we also conducted the following:

 Monitoring of clonal selection fields in Quinta da Granja in Alijó - property of member "Companhia Geral da Agricultura do Norte" during grafting and data collection for the vintage.

 Collection of field rods for clone selection in the RDD to transfer to the experimental hub of Pegões.

 Implementation and monitoring of two Tinta Roriz variety selection fields using grafting technology in order to establish a more expeditious method of obtaining results. Clone material was collected and the experimental design was elaborated.

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6.1.6 Sustained Viticultural Production

The action and promotion plans were developed by ADVID and Vinideas, Ltd. Materials were supplied by ADVID for the VINIDEAS platform;

Information collection on reference plots according to the model prepared by ADVID, following the plot structure by utilisation levels according to the execution model discussed with associate members, who agreed with the information collection;

Information collection and processing to support research activity and experimentation, decision-making by winemakers, plant protection strategies for vineyards, soil conservation and vegetation maintenance.

Predictions of the harvest potential in the Douro Region were made, which were based on the pollen project results in collaboration with the IVDP and College of Sciences, University of Porto.

The study "Effects of rootstock and environment on Portuguese grape varieties in the Douro region" was developed and a paper was presented at the IX Congrés International Terroirs Vitivinicoles (Dijon/Reims).

The communication "Prediction method of harvest in Douro" was presented during the seminar "Technological innovations in grapevines and wine", which was included in the II Wine Meetings-Vinho Verde, as organised by the Order of Engineers - College of Agricultural Engineering.

"Production Forecast for 2012 vintage" workshop, Régua, 12 July. "Importance of controlling ripening grapes" workshop. Régua, 25 July.

"Viticultural Year 2012 - Balance" workshop. Solar do IVDP, Régua, 3 December.

6.1.7 Rationalisation of the hillside vineyard

Following approval of the application submitted to the SIAC called "Rationalisation of hillside vineyards - Mapping of landslides", the main objective of which is based on the elaboration of mapping to support risk identification and support investment decisions by enterprises, information-gathering devices were initiated.

In addition to the compilation and processing of climate information, an event log and GIS information were prepared to support the project.

Meetings were held with the Department of Physical Geography, University of Porto, to refine the methodologies for mapping the susceptibility to slope movements.

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6.1.7.1 Economic Observatory

This study was used to establish the Economic Observatory for the School of Economics at the University of Porto, and the necessary methodology was defined, with particular attention to the "Development of a Composite Model for Determining the Value of the Grapevine" and "Web Deployment Model of Valuation of the Grapevine.”

6.1.8. Skills Development - Training and Dissemination

“Ampelography - Course II” (56 h)

In response to the success of the ampelography courses provided in 2010 and 2011, ADVID conducted "Ampelography - Course II" in 2012.

This course focused on the ampelographic identification and characterisation of the grape varieties recommended for the RDD. This course used the Ampelographic Collection of Quinta de Santa Barbara, visits to the National Ampelographic Collection in Dois Portos (INRB) and the Experimental Centre for Conservation of Native Variability of Vines by PORVID (Portuguese Association for Grapevine Diversity), in Pegões.

Mr. Mário Cardoso (DRAP-N) was invited to instruct the course, which also had the support of the IVDP classifiers.

Photo 1 – Field session to characterise ampelographic grape varieties. Photo 2 – The course instructor, Mr. Mario Cardoso (DRAP-N)

Training courses for winegrowers

Application to POPH, Measure 2.3. - Certified Modular Training with training in Agricultural and Animal Production, was submitted in late 2011, and it was approved in the second half of 2012, with a total of 14250 training hours. It began in July 2012, and various courses had already been completed in 2012. The three courses are described below.

Table 2 –POPH courses, conducted in 2012

Course Period Length Number of

graduates Pruning and grafting in viticulture 11-07 to 27-12-2012 50 h 16 Plant protection product application 23-11 to 7-11-2012 65 h 17 Regulation, operation and maintenance of machinery and equipment 13-11 to 6-12-2012 50 h 19

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Training Courses for Technicians

For PRODER, we promoted courses called "Integrated Production Vineyard" (168 h) and "General Organic Agriculture" (140 h) to target technicians from the viticulture sector.

The campaign "Building a Capacity Network for Promoting Employability - Douro” was submitted to “Promotion and Institutional Capacity Building - Institutional Networks 2010 - Axis V", which had the objective of promoting employability in the Douro region in 2012. The organisations promoting the project, namely DREN and IEFP, were restructured and they left both the campaign and the project.

We are still waiting for a decision on the application for ON2 funding, which was submitted in Feb. 2011 and entitled "Continuous Assessment of the Technological Status of Viticulture in the Douro Region".

Photo 3 - Graduates of the Course in Organic Agriculture. Photo 4 - Graduates of Integrated Production in the Vineyard.

6.2. Complementary R&D Projects

6.2.1. Evaluating the effectiveness of treatments to combat grapevine

Oidium

at

early stages of the vegetative cycle.

We continued to test a plot located in the Cima Corgo with the Tinta Roriz grape variety. The goal was to evaluate the treatment efficacy during early phases of the vegetative cycle. Treatments were positioned between bud burst and pre-flowering (14 days) to control the eventual primary infections during a powdered sulphur shortage that resulted from low temperatures (low efficiency of sulphur powder). Four treatments were applied: 1 - Control; 2 - Application of 3 treatments with wettable sulphur before pre-flowering; 3 - Foliar treatments during the vegetative phase and another during pre-flowering; 4 - Only one treatment in the pre-flowering stage with an IBE. The attack intensity within the vintage was evaluated by recording the incidence and severity of damaged bunches

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Table 3 - Current Status of Anchor Projects

Flagship Projects Typology Current situation Observations

P ro po sed funding 163,582.00 P ro po sed funding 28,138.00

Eligible funding 163,582.00 Eligible funding 28,138.00

Financial Suppo rt 163,582.00 Financial Suppo rt 28,138.00

P ro po sed funding 50,000.00 P ro po sed funding 50,000.00

Eligible funding 37,500.00 Eligible funding 37,500.00

Financial Suppo rt 37,500.00 Financial Suppo rt 37,500.00

M o deling the Evo lutio n o f RDD Wine Quality. P hD pro ject fro m FEUP .

PhD f rom FEUP. M odelling t he evolut ion of RDD wine qualit y.

Ongoing, A DV ID

f unding __ 9,000.00

__

9,000.00

2. Zo ning o f Viticultural P o tential o f

the Do uro Demarcated Regio n P RODER In preparat ion. Submit in 2013.

Ongoing, A DV ID

f unding __

__

P ro po sed funding 1,951,043.00 P ro po sed funding 109,602.00

Eligible funding 1,951,043.00 Eligible funding 109,602.00

Financial Suppo rt 959,523.00 Financial Suppo rt 54,801.00

9,000.00 9,000.00

P ro po sed funding 1,042,618.18 P ro po sed funding 206,544.97

Eligible funding 647,248.64 Eligible funding 132,920.01

Financial Suppo rt 420,712.35 Financial Suppo rt 86,398.01

4. B io diversity P reservatio n o f Grapevine Varieties

P RODER – A ctio n 2.3.1 Co nservatio n and Impro vement o f Genetic Reso urces

Approved. PA no. 18661. Leader ent ity dropped out and t he PA was annulled.

Ongoing. A DV ID f unding

P ro po sed funding 388,662.06 P ro po sed funding 189,678.12

Eligible funding 293,552.02 Eligible funding 139,088.26

Financial Suppo rt 220,566.27 Financial Suppo rt 104,316.2

Submit ted. Under analysis Submit ted. Under analysis

P ro po sed funding 149,401.48 P ro po sed funding 149,401.48

Approved SIAC 23300 Eligible funding 119,650.62 Eligible funding 119,650.62

Financial Suppo rt 83,755.43 Financial Suppo rt 83,755.43

SIA C Transferred from Regional to Nat ional

SIA C in 2013

P ro po sed funding 128,524.35 P ro po sed funding 128,524.35

Eligible funding 110,444.65 Eligible funding 110,444.65

Financial Suppo rt 110,444.65 Financial Suppo rt 110,444.65

ON2 – M easure – “ Institutio nal P ro mo tio n and Capacity B uilding – Institutio nal Netwo rks 2010 – A xis V. “ Institutio nal Capacity B uilding Netwo rk fo r P ro mo ting Emplo yability - Do uro

Approved

Lead organisat ion wit hdrew and t he PA was cancelled

7º P Q -Intelligent Service Oriented M anagement System fo r P recisio n Viticulture (IM P ROVE). A pplicatio n Sto re

fo r SM ES 7º P Q Intelligent Service Oriented M anagem ent System fo r P recisio n Viticulture (VEGSTORE) - A pplicatio n Sto re fo r GM ES Vegetatio n Services

___

M aximising Vineyard Eco system Services. P RODER, M ed 4.1-Co o peração para a Ino vação

Approved PA 24042 Ongoing, PRODER f unding

8. Co mpetency Develo pment – Training and Disseminatio n

P OP H, M easure 2.3. – Certified M o dular

Fo rmatio ns Approved. PA no. 075638/ 2012/ 23 Ongoing

6. Sustainable Viniculture P ro ductio n

P RODER, A ctio n 4.2.2 – Info rmatio n and

Disseminatio n Them atic Netwo rks Approved. PA no. 14368

Ongoing. PRODER f unding

7. Rasio nalisatio n o f

Implementatio n o f Hillside Vineyard SIA C Ongoing. COM PETE f unding

5. A ssessment o f Oeno lo gical

A ptitude o f Grapes SA ESCTN (FCT) iGrape Submit ted. Under analysis

3. . Functio nal B io diversity in Viticulture

B io DiVine (Life+ Nature and B io diversity 2009)

Ongoing. Project LIFE9 NA T/ FR/ 000584.

Part nership with 7 ent it ies f rom 3 count ries. Lif e+ f unding ADVID f unding

Eco lo gical infrastructures in the bio lo gical pro tectio n o f co nservatio n against vineyard pests. P hD pro ject fro m A DVID/UTA D.

Ongoing. PhD f rom UTAD ADVID f unding

Total funding

___

ADVID funding

1. 1. Impact o f Climate Change o n Viticulture o f the Do uro Demarcated Regio n. M anagement o f water relatio ns.

SA ESCTN – Sho rt-term climate change mitigatio n strategies in M editerranean viticulture (ClimVineSafe);

Approved. Project no. º PTDC/A

GR-ALI/ 110877/ 20 Ongoing. FCT f unding

Climate assessment in the RDD (SIA C); Parcial support SIA C - 7607 Concluded, COM PETE +

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7.

DISSEMINATION, COMMUNICATION AND TRAINING

7.1.

Production of informational support material

The following informational support material was produced in 2012:

7 lists of plant protection products approved for integrated production

15 newsletters (bulletins)

10 leaflets about phytosanitary monitoring and other information

Table 4 – Lists, newsletters and leaflets produced in 2012.

List of Plant Protection Products Approved for Integrated Protection Date

List of approved herbicides for IP - 2012 19-Jan

List of anti-Phomopsis fungicides approved for IP - 2012 16-Mar List of anti-mildew fungicides approved for IP- 2012 4-Apr List of anti-powdery mildew fungicides approved for IP - 2012 4-Apr List of insecticides and acaricides approved for IP - 2012 19-Jun List of black rot fungicides approved for IP - 2012 19-Jun  List of Botrytis bunch rot fungicides - 2012 10-Jul

Newsletters Data

Newsletter 01 - Training Balance 12-Jan

Newsletter 02 - Herbicides - Support Elements for Use in IP 24-Feb

Newsletter 03 – Conditionality Rules March 2012

Newsletter 04 - Grapevine Integrated Production - Technical Guidelines March 2012 Newsletter 05 - Integrated Production Mode (IPM) March 2012

Newsletter 06 – Organic Production Mode (BPM) March 2012

Newsletter 07- Integrated Territorial Intervention in the Douro Wine Region - ITI DV March 2012 Newsletter 08- Single Payment Scheme (SPS) - General Principles March 2012 Newsletter 09- Maintenance of Agricultural Activity in Less Favoured Areas - MLFA March 2012

Newsletter 10 - Help with Wine Distillation March 2012

Newsletter 11 - Supplements for Olive Oil and Table Olives March 2012

Newsletter 12 - Analyses of Soil and Plants 22-May

Newsletter 13 - Black Rot - Support Elements for Identification and Control 31-May Newsletter 14 - Viticultural Year 2012- Forecast Harvest Potential 12-Jul

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Pamphlets about phytosanitary monitoring and other information Date Pamphlet 01-12 - Evolution of Drought - Harvest Year 2011-2012 1-Mar Pamphlet 02-12 - Evolution of Mildew and Powdery Mildew 24-Apr Pamphlet 03-12 - Evolution of Mildew and Powdery Mildew in the RDD 8-May Pamphlet 04-12 - A New Period of Empty Container Collection 14-May Pamphlet 05-12 - Evolution of Mildew and Powdery Mildew in the RDD 22-May Pamphlet 06-12 - Evolution of Powdery Mildew, Black Rot and Mildew. Foliar Analysis IP 5-Jun Pamphlet 07-12 - Powdery Mildew, Black Rot, Moths and Leafhoppers 18-Jun Pamphlet 08-12 - Powdery Mildew, Mildew, Grapevine Moth and Leafhopper 2-Jul

Pamphlet 09-12 - Maturation Analyses 23-Jul

Pamphlet 10-12 - Grapevine Moth and Leafhopper 14-Aug

ADVID also produced the following:

 Brochures to disseminate information about the BIODIVINE product (Life+) - Demonstration of functional biodiversity in vineyard landscapes.

 The book "A Climate Assessment for the Douro Wine Region: An Examination of the Past, Present and Future Conditions for Wine Production", which presents the results of the project “Climate changes and wine production”.

 Technical Bulletin No. 5 - Powdery Mildew of the Grapevine

Figure

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References

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