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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

H2020 MSCA-ITN 2016

PATHSENSE

D6.4 Annual completion of Network-wide

workshops & training events

Responsible partner: UR

Authors: Nina L Tuite and Conor O Byrne, National University of Ireland, Galway; Bert Poolman, University of Groningen; Nick Johnson, Nestle.

Coordinator: Conor O Byrne, National University of Ireland, Galway. Project website: www.pathsense.eu

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

Project acronym PATHSENSE

Project full title Training Network to Understand and Exploit Mechanisms of Sensory Perception in Bacteria

Grant Agreement no. 721456

Project start date 1-04-2017

Deliverable Title D6. Annual completion of Network wide workshops & training events yr2

Work Package Number WP 6

Work Package Title TRAINING

Lead beneficiary WP6 UR

Author(s) NUI Galway; RUG; Nestle.

Date Due 31 March 2019

Date of Submission 28 March 2019

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

Table of Contents

Executive Summary ... 4

PATHSENSE Background ... 4

1. Workshop 3 - Scientific Integrity & Ethics ... 5

1.1 INTRODUCTION ... 5

1.2 OVERVIEW OF WORKSHOP ... 6

1.3 TYPES OF MISCONDUCT: ... 6

1.4 CONCLUDING REMARKS ... 7

2. Workshop 4 - The Power of Microscopy: Seeing is Believing. ... 8

2.1 MICROSCOPY MASTERCLASS 1 ... 8

2.2 MICROSCOPY MASTERCLASS 2 ... 9

3. Summer School 1 - Translating Postgenomic Microbiology to Advancing Food Science & Safety .. 11

3.1 DAY 1 – INTERNAL AND EXTERNAL SPEAKERS ... 11

3.2 PART 2, DAY 2 - POPULAR SCIENCE COMMUNICATION – FOOD SAFETY MICROBIOLOGY ... 12

4. International Conference and Symposium. ... 14

4.1 EUROPEAN FEDERATION OF BIOTECHNOLOGY (EFB) 4TH MICROBIAL STRESS MEETING, KINSALE, CORK, IRELAND. 23RD – 25TH APRIL 2018. ... 14

4.2 LIST_MAPS SYMPOSIUM EFB SATELLITE MEETING - INTERNATIONAL SYMPOSIUM FOODBORNE PATHOGENS: FROM FARM TO PHARMACY! KINSALE, CORK, IRELAND. THURSDAY 26TH APRIL 2018. ... 16

5. PATHSENSE Network Review meeting - ESR presentations and discussion session ... 18

6. Feedback from ESRs regarding Workshop 3&4 and Summer School 1 ... 20

7. Conclusions and planning of the next training events ... 22

7.1 CONCLUSIONS ... 22

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

Executive Summary

The purpose of this document is to report on Work Package 6 deliverable 6.4, the Network-wide workshops and training activities during month 13-24 of PATHSENSE Project. The 13 Early Stage Researchers (ESRs) are currently 16 - 19 months into their projects. In that time they have experienced two Network Wide Training events. Described here is Workshop 3 ‘Scientific Integrity & Ethics’; Workshop 4

The Power of Microscopy: Seeing is Believing’; Summer School 1 ‘Translating Postgenomic Microbiology to Advancing Food Science & Safety’, the Network Review meeting and the scheduling of the next Network training events.

PATHSENSE Background

The PATHSENSE (Pathogen Sensing) ETN brings together an interdisciplinary team of world-leading researchers from Europe to tackle a highly ambitious scientific project, focusing on the molecular mechanisms of sensory perception in bacterial pathogens. PATHSENSE has established an innovative doctoral training programme that will deliver 13 PhD graduates and high-impact scientific outputs. The relationship between molecular structures and biological function is central to understanding any living system; however the research methodologies required to unravel these relationships are often complex and fast-changing.

The team participating in this Network has the infrastructure and track-record to train ESRs in these state-of-the art methodologies, including structural biology, proteomics & protein biochemistry, molecular biology, bacterial genetics, food microbiology, mathematical modelling, cell biology, microscopy and comparative genomics. PATHSENSE will investigate the poorly understood structure function relationships that exist within a large multi-protein complex called a “stressosome”, which acts as a sensory organelle in bacteria.

The project will involve extensive inter-sectoral mobility of the ESRs across 7 EU countries to make full use of the complementary skills available at each of the hosting institutions. The inter-sectoral Network comprises 10 leading Universities, 1 public research institution, 4 companies (from spin-off to large multi-national) and 1 governmental agency.

A major objective of this Network will be to exploit the fundamental research to develop novel antimicrobial treatments that have applications in the food and public health sectors. This project will deliver high-impact science, 13 highly-trained innovative researchers and will produce a long-lasting inter-sectoral network of collaborators who will continue to work together to exploit fundamental research for the socio-economic benefit of Europe. See more at www.pathsense.eu

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

1. Workshop 3 - Scientific Integrity & Ethics

Dates: Tuesday, 12th–Wednesday 13th June 2018.

Duration: 1 day (split between 2 days) External Trainers:0

Internal Trainers: 1

1.1

INTRODUCTION

As part of their Network-wide training the PATHSENSE ESRs took part in an interactive workshop on Ethics and Integrity in Science, delivered by Professor Bert Poolman from the University of Groningen in the Netherlands.

The overall aim of this Workshop is to train the PATHSENSE ESRs be responsible and independent researchers. Following a seminar style information session, the ESRs deliberated over a of number case studies and held interesting discussions about how to recognise and avoid scientific mis-conduct, considering issues such as plagiarism, duplication, allocation of credit, falsification of data and conflicts of interest.

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

1.2 OVERVIEW OF WORKSHOP

Trainer: Prof. Bert Poolman

1.3 TYPES OF MISCONDUCT:

The following types of misconduct were highlighted, explained and discussed in detail: - Plagiarism (self-plagiarism)

- Duplication of publication - Allocation of credit

- Fabrication/Falsification of data - Conflicts of Interest/Peer Review

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

1.4 CONCLUDING REMARKS

This workshop was successful in raising awareness of ethics and integrity and the importance of such amongst the ESRs and was thought provoking at all stages. Some feedback from the ESRs is shown in section X. Following this Workshop one of the ESRs wrote a comprehensive blog post entitled “An essential book about scientific integrity for ESR: it’s ‘fantastic’”, this can be read here.

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

2. Workshop 4 - The Power of Microscopy: Seeing is Believing.

Wednesday and Thursday, 14th & 15th June 2018.

Duration: 2 days External trainers: 1 Internal trainers: 2

The PATHSENSE ESRs took part in the Network-wide training Workshop 4 – The power of Microscopy. This workshop took place over two days in June 2018 and was run by Prof Bert Poolman of the University of Groningen, NL. This neatly designed module covered range of topics such as the principals of microscopy methods; use and (dis)advantages of fluorescent proteins and dyes; single molecule analysis etc as outlined below. The ESRs also were tasked with a number of assignments in order to become familiar with various microscopy methods. With these practical sessions, some aspects of the course (theory) were practised on the microscope (imaging of cell, measurements on protein diffusion, bleaching of fluorophores).

The microscopy workshop was broken down into two Masterclasses. Shown below is the topics, learning objectives and literature associated with Masterclass 1 & 2.

2.1 MICROSCOPY MASTERCLASS 1

Fluorescence Microscopy I

- principles of microscopy - principles of fluorescence

- fluorescent proteins, dyes and quantum dots

- conventional fluorescence versus confocal microscopy - fluorescence correlation spectroscopy

Learning objectives:

- Can explain the basics of optical microscopy and fluorescence

- Can explain basic optical microscopy methods: confocal microscopy, super-resolution imaging, single-particle tracking etc.

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”. Literature

- Lakowicz JR (2006) Principles of Fluorescence Spectroscopy, Springer Verlag

- Philips et al (2013) Physical Biology of the Cell (2nd edition), Chapter 13 “A statistical view of Biological Dynamics”

- Alberts et al (2015) Molecular Biology of the Cell (6th edition), Chapter 9 “Visualizing cells”; pp529-553

- Bacia & Schwille (2002) Methods 29, 74

2.2 MICROSCOPY MASTERCLASS 2

Fluorescence Microscopy II

- Why single molecule analysis? - Bleaching step analysis

- Confocal microscopy versus Total Internal Reflection Fluorescence Microscopy (TIRF) - Super-resolution microscopy: PALM, STORM

- Photoswitchable proteins and dyes - Lateral diffusion

- Fluorescence Recovery after Photobleaching (FRAP) - Single-Molecule Tracking (SMT)

Learning objectives:

- Can explain the principles of fluorescence-based microscopy methods: confocal imaging, FRAP, FRET, FCS, SRM, SMT

- Can apply knowledge of fluorescence-based microscopy to analyze the structure and dynamics of the cell

- Can quantitatively analyze diffusion in 3D and 2D, and use this property to characterize the structure of the cytoplasm and membranes

Literature

- Alberts et al (2015) Molecular Biology of the Cell (6th edition), Chapter 9 “Visualizing cells”; pp529-553

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”. - Mika & Poolman (2011) Macromolecule diffusion and confinement in prokaryotic cells. Curr Opin

Biotech 22, 117

- Lakowicz JR (2006) Principles of Fluorescence Spectroscopy, Springer Verlag - Ramadurai et al (2009) J Am Chem Soc 131,12650

- Saffman & Delbrück (1975) PNAS USA 72, 3111

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

3. Summer School 1 - Translating Postgenomic Microbiology to Advancing

Food Science & Safety

Dates: Tuesday, 12th–Wednesday 13th June 2018.

Duration: 2 day External Trainers:8 Internal Trainers: 5

This summer school was reported to the EU in detail in the form of Deliverable D6.3 Summer school 1, shown here is a brief overview. The goal of the PATHSENSE Summer schools is to focus on the latest research and make new research links with leading scientists external to the Network. As part of these summer schools, leading researchers in their fields are invited to give research talks and lead discussion groups with the ESRs. SS1 focussed on how the latest advances in big-data biology can be exploited by food scientists to develop innovative strategies to enhance food safety and quality and improve shelf-life. The aim is for the to enhance the training of ESRs by further developing their critical thinking, networking and communication skills, as well as helping them to develop the bigger-picture view of their research areas.

Summer School 1 took place on June 12th – 13th 2018 as part of the network-wide training in the PATHSENSE Project. Summer School 1 “Translating Postgenomic Microbiology to Advancing Food Science & Safety” was designed as a 2-day course with invited and internal speakers covering topics in food microbiology including problems of food spoilage and food safety, EU regulations, innovative approaches to improving food safety, and developing new antimicrobial preservation regimes. It also included a 1 day session on Popular Science Communication, with an emphasis on Food Safety Microbiology.

3.1 DAY 1 – INTERNAL AND EXTERNAL SPEAKERS

The aim was to introduce the ESRs to the wider applications and technologies of their specific scientific studies (particularly those related to genomics) and that of the PATHSENSE project in general. Apart from imparting knowledge concerned directly to the general stress response, the intention was also to give the ESRs insights and opportunities outside their direct fields associated to academia and microbial stress sensing; i.e. potential for future careers in food authorities, food industry and technology companies, and medicine, where the translation and post-genomic applications have also becoming established as critical tools.

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”. Speaker 1: Dr. Nicholas Johnson Nestlé S.A. (Switzerland) - Introduction

Speaker 2: Tjakko Abee - Wageningen University - Plant-derived antimicrobials: structures and mode of action

Speaker 3: Jan Kok - University of Groningen – The early transcriptome response of Lactococcus

lactis to environmental stresses

Speaker 4: Daniel Hurley (University College Dublin) – Factory ecology and microbiomics

Speaker 5: Jos Boekhorst (NIZO) - Applications of NG Omics and Bioinformatics to identify candidate genes, strains and biomarkers

Speaker 6: John Rossen (Medical Microbiology, UMCG) – Medical aspects of microbial detection and identification of bacteria

Speaker 7: Dr. José Carlos Quintela – (Natac Biotech, Spain) - Botanical based compounds with antimicrobial properties, from the bench to the market.

Speaker 8: Marjon Wells-Bennik (NIZO) – Application of Omics in Hazard Characterisation

Speaker 9: Dr. Herman de Vries (BioClear Earth) – Insight from a Spin-Out in application of Omics in microbial analysis

Speaker 10: Lisa O’Connor (FSAI) - Application of Omics; a regulatory perspective Speaker 11: John Donaghy (Nestlé) - Application of Omics; an industry perspective

Photo from Summer school Day 1, Groningen NL, June 2018.

3.2 PART 2, DAY 2 - POPULAR SCIENCE COMMUNICATION

– FOOD SAFETY MICROBIOLOGY Trainer: DR STEVEN FLIPSE FROM HERTZ TRAINING

Popular Science Communication

The ability to communicate efficiently with non-experts is a very important skill for the 21st century scientist. Through this training session, the PATHSENSE ESRs developed skills on how to communicate with the general public through popular scientific writing. They learned of the need to be concise, to

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”. use well-chosen examples, and to begin with the most important findings of one’s research without the use of jargon. Participants learned how to present their research in a way that is easily understood and absorbed by non-scientists. The layout of this session mixed theory and practice with examples of good popular science communication. Topics covered were (i) Differences between academic and popular writing; (ii) Structure / lay-out; (iii) Use of metaphors and style and (iv) Storytelling. The 13 participants, working in four groups, were assisted to author an article each on a topic around ‘translating omics to food safety and / or health’. Four articles were created, one of which has so far has been published in the November 2018 issue of the University of Dundee student magazine, Magdalen. This is a freely accessible magazine, copies of which are also kept in the university library.

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

4. International Conference and Symposium.

23rd - 26th April 2018.

Duration: 2 days

As part of the PATHSENSE Network-wide training, 12 of the 13 ESRs attended the European Federation of Biotechnology (EFB) 4th Microbial Stress meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018 and the List_Maps Symposium EFB satellite meeting - International Symposium Foodborne pathogens: from farm to pharmacy! Kinsale, Cork, Ireland. Thursday 26th April 2018.

4.1 European Federation of Biotechnology (EFB) 4

th

Microbial Stress meeting, Kinsale,

Cork, Ireland. 23

rd

– 25

th

April 2018.

This conference gathered scientists working in fundamental and applied research who are interested in understanding how microorganisms cope with stress. The meeting welcomed microbiologists, biotechnologists, system biologists, biochemists and molecular biologist from both academia and industrial sectors: http://www.efbiotechnology.org/microbialstress/ . The PATHSENSE Coordinator Conor O’ Byrne was one of the organisers of this Conference.

11 of the individual research projects were presented at this conference in April 2018. Below lists the titles of poster presentations that were presented. ESR8, Ana Oliveira delivered an oral presentation based on her poster. In addition PATHSENSE Principal Investigators Conor O’Byrne and Jorgen Johansson were also speakers at this event.

Photo of Conference attendees including PATHSENSE participants and banner for Microbial Stress

Titles of posters presented at the European Federation of Biotechnology (EFB) 4th Microbial Stress meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018.

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”. Manisha Pandey and Nicola Stanley-Wall. Genetic study of the molecular mechanisms of sensory perception in Bacillus subtilis. European Federation of Biotechnology (EFB) 4th Microbial Stress

meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018.

Charlotte Dessaux, M. Graciela Pucciarelli and Francisco García-del Portillo. Detecting Listeria monocytogenes stressosome proteins in response to stress. European Federation of Biotechnology

(EFB) 4th Microbial Stress meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018.

Laura Cutugno, Maria Conway, Jan Pané-Farré and Aoife Boyd. Genetic characterisation of the Vibrio

vulnificus stressosome and its role in virulence. European Federation of Biotechnology (EFB) 4th

Microbial Stress meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018.

Maria Conway, Kristina Wickes, Laura Cutugno, Sema Ejder, Algirdas Mikšys, Aoife Boyd, Christina Ziegler, Rick Lewis, Jon Marles-Wright, Mareike Fischer and Jan Pané-Farré. Phylogenetic distribution of RsbR orthologs and sequence conservation in stressosome proteins and their putative output modules. European Federation of Biotechnology (EFB) 4th Microbial Stress meeting, Kinsale, Cork,

Ireland. 23rd – 25th April 2018.

Inge van Vilsteren and Nicholas Brian Johnson. Influence of osmotic stress on heat resistance in food products. European Federation of Biotechnology (EFB) 4th Microbial Stress meeting, Kinsale, Cork,

Ireland. 23rd – 25th April 2018.

KahYen C. Yeak, Jos Boekhorst, Tjakko Abee, Marjon H.J. Wells-Bennik. Distribution of Sigma B stressosome and RsbK signalling pathways and predicted regulons in the genus Bacillus. European

Federation of Biotechnology (EFB) 4th Microbial Stress meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018.

E. Ivanova Stojcheva, E. de la Fuente, F. García-del Portilloand J.C. Quintela. Antilisterial activity

of selected plant extracts in unstressed conditions and in combination with sublethal stresses of pH, temperature and NaCl. European Federation of Biotechnology (EFB) 4th Microbial Stress meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018.

Algirdas Miksys, Veronika Heinz, M. Gregor Madej, Susann Kaltwasser, Jan Pané-Farré, Maria Conway, Rick Lewis, Jon Marles-Wright and Christine Ziegler. Investigation of symmetry breaks in the assembly of the stress-sensing stressosome complexes of L. monocytogenes and V. vulfnificus by cryo-EM.

European Federation of Biotechnology (EFB) 4th Microbial Stress meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018.

Sema Ejder, Jon Marles-Wright and Rick Lewis. Structure-function relationships of the stress sensory domains in the Listeria monocytogenes stressosome. European Federation of Biotechnology (EFB) 4th

Microbial Stress meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018.

Ana Oliveira, Teresa Tiensuu, and Jörgen Johansson. The role of RsbX in the stressosome sensory system of Listeria monocytogenes. European Federation of Biotechnology (EFB) 4th Microbial Stress

meeting, Kinsale, Cork, Ireland. 23rd – 25th April 2018.

Duarte Guerreiro and Conor O’Byrne. Genetic and phenotypic characterization of the sensory proteins in the Listeria monocytogenes stressosome. European Federation of Biotechnology (EFB)

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

4.2 List_Maps Symposium EFB satellite meeting - International Symposium Foodborne

pathogens: from farm to pharmacy! Kinsale, Cork, Ireland. Thursday 26th April 2018.

This symposium was organized by the H2020 funded MSCA ITN project List_MAPS GA no. 649184. http://blog.u-bourgogne.fr/list-maps/conference/.

12 of the PATHSENSE Early Stage Researchers presented their research at this event. Below lists the titles of posters that were presented at the List_Maps Symposium. ESRs 4 & 6 also had the opportunity to deliver oral presentations at this symposium (see below). The PATHSENSE ESRs also had a chance to network with PIs and ESRs from a late-stage ITN project and valuable connections were made and information exchanged.

List_Maps Logo and photo showing PATHSENSE ESRs during networking session at List_Maps Symposium

Titles of posters and talks presented at the List_Maps Symposium EFB satellite meeting - International Symposium Foodborne pathogens: from farm to pharmacy! 26th April 2018.

Manisha Pandey and Nicola Stanley-Wall. Genetic study of the molecular mechanisms of sensory perception in Bacillus subtilis. List_Maps Symposium EFB satellite meeting - International Symposium

Foodborne pathogens: from farm to pharmacy! 26th April 2018.

Charlotte Dessaux, M. Graciela Pucciarelli and Francisco García-del Portillo. Detecting Listeria monocytogenes stressosome proteins in response to stress. List_Maps Symposium EFB satellite

meeting - International Symposium Foodborne pathogens: from farm to pharmacy! 26th April 2018. Poster and oral presentation

Laura Cutugno, Maria Conway, Jan Pané-Farré and Aoife Boyd. Genetic characterisation of the Vibrio

vulnificus stressosome and its role in virulence. List_Maps Symposium EFB satellite meeting -

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”. Maria Conway, Kristina Wickes, Laura Cutugno, Sema Ejder, Algirdas Mikšys, Aoife Boyd, Christina Ziegler, Rick Lewis, Jon Marles-Wright, Mareike Fischer and Jan Pané-Farré. Phylogenetic distribution of RsbR orthologs and sequence conservation in stressosome proteins and their putative output modules. List_Maps Symposium EFB satellite meeting - International Symposium Foodborne

pathogens: from farm to pharmacy! 26th April 2018. Poster and oral presentation

Inge van Vilsteren and Nicholas Brian Johnson. Influence of osmotic stress on heat resistance in food products. List_Maps Symposium EFB satellite meeting - International Symposium Foodborne

pathogens: from farm to pharmacy! 26th April 2018.

KahYen C. Yeak, Jos Boekhorst, Tjakko Abee, Marjon H.J. Wells-Bennik. Distribution of Sigma B stressosome and RsbK signalling pathways and predicted regulons in the genus Bacillus. List_Maps

Symposium EFB satellite meeting - International Symposium Foodborne pathogens: from farm to pharmacy! 26th April 2018.

E. Ivanova Stojcheva, E. de la Fuente, F. García-del Portilloand J.C. Quintela. Antilisterial activity

of selected plant extracts in unstressed conditions and in combination with sublethal stresses of pH, temperature and NaCl. List_Maps Symposium EFB satellite meeting - International Symposium Foodborne pathogens: from farm to pharmacy! 26th April 2018.

Algirdas Miksys, Veronika Heinz, M. Gregor Madej, Susann Kaltwasser, Jan Pané-Farré, Maria Conway, Rick Lewis, Jon Marles-Wright and Christine Ziegler. Investigation of symmetry breaks in the assembly of the stress-sensing stressosome complexes of L. monocytogenes and V. vulfnificus by cryo-EM.

List_Maps Symposium EFB satellite meeting - International Symposium Foodborne pathogens: from farm to pharmacy! 26th April 2018.

Sema Ejder, Jon Marles-Wright and Rick Lewis. Structure-function relationships of the stress sensory domains in the Listeria monocytogenes stressosome. List_Maps Symposium EFB satellite meeting -

International Symposium Foodborne pathogens: from farm to pharmacy! 26th April 2018.

Ana Oliveira, Teresa Tiensuu, and Jörgen Johansson. The role of RsbX in the stressosome sensory system of Listeria monocytogenes. List_Maps Symposium EFB satellite meeting - International

Symposium Foodborne pathogens: from farm to pharmacy! 26th April 2018.

Duarte Guerreiro and Conor O’Byrne. Genetic and phenotypic characterization of the sensory proteins in the Listeria monocytogenes stressosome. List_Maps Symposium EFB satellite meeting -

International Symposium Foodborne pathogens: from farm to pharmacy! 26th April 2018.

Torkel Loman and James Locke. Mathematical Modelling as a Tool to investigate environmental stress response in B. Subtilis. List_Maps Symposium EFB satellite meeting - International Symposium

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”. Photos of Networking and Poster sessions at the conference and symposium April 2018.

5. PATHSENSE Network Review meeting - ESR presentations and discussion

session

During the Network review meeting, each of the 13 ESRs delivered a 15 min presentation on their individual research projects to the network. Each presentation was followed by a question and answer session. This session is considered part of their training since the students are experiencing and learning how to effectively communicate their research and engage in useful scientific discussion with an audience of their peers and experts in their field. Useful and fruitful discussions ensue. Important

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”. feedback is provided from Network members, which help the students to refine their project plans at this early stage of the project.

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

6. Feedback from ESRs regarding Workshop 3&4 and Summer School 1

The PATHSENSE ESRs were asked for feedback from Workshop 3 & 4 and Summer School 1. The feedback received in the points below will be taken into consideration for the next training event.

What did they most enjoy about the training week in Groningen?

Some of them expressed more than one preference, I tried to summarize them in a table (each bar represents a preference).

As you can see the Ethics and Microscopy workshop were the most appreciated ones. In general everyone really enjoyed the opportunity to meet and discuss with the other ESRs and PIs.

What did they least enjoy?

In this case someone didn’t mention any activity in particular. Here the table with the feedback.

I would like to explain that in the specific case of the Popular Scientific Writing most of the people found the content of the workshop really interesting and everyone found Steven Flipse to be a really good instructor. The main problem was the lack of time. It was really intense and I would say poorly productive to do everything in 6 hours, especially because we were asked to produce the first draft of an article by the end of the day.

Overall was the level of challenge/difficulty to hard, too easy or just right?

For everyone the level of challenge/difficulty was just right

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”. As I’ve already said most of us found the schedule too intense (only one person didn’t agree on this point). Every workshop/talk/activity was potentially really interesting but we didn’t have enough time to discuss and “metabolize” all the information we got.

I report verbatim some of the comment I received from the ESRs:

“Too much information in a too short time to properly learn something. Really nice topics/workshops. Don't compress the information in to short time”

“So overall, even though I am extremely grateful about the training we received and I very much enjoyed most of it, I believe this whole trip needed to be two more days longer”

“Lesser lectures but intensively discussed can be better. For instance, on the second day, most lecturers were presented in a hurry manner, some lectures were really good but just did not have sufficient time”

“Content of the public writing workshop was good but since we were asked to perform on such a short notice I don't know if we were able to absorb enough”

“It was not too difficult. I think it was generally a good idea to keep a high pace. When we have travelled for the workshop it feels good to ensure we get as much as possible out of it”

All of the feedback received will be carefully considered when planning the next Network-wide training events.

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“This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 721456”.

7. Conclusions and planning of the next training events

7.1 Conclusions

Overall the Network-wide training that was organised and held between Month 13 and Month 24 of the PATHSENSE project has been a success and consisted of two workshops (Workshop 3 &4), a Summer School (SS1), an International Conference and Symposium and a Network review meeting. In addition 7 of the PATHSENSE ESRs have completed their first secondment.

7.2 Schedule of THE NEXT NETWORK-WIDE Training EVENTS

Summer Schools and Workshops – June 2019

We will also be holding the next Network review meeting and Workshop 8 to coincide with the Mid-term Review meeting in April 2019. Our second Summer school and Workshops 5 & 6 are currently being planned for June 2019, in Newcastle, UK.

Network wide Training events scheduled for the coming months

Title Venue Date Responsible

Partner

Type(s) of Skills/Knowledge Work Shop 8: Developing a

Research Proposal: Grant Writing Skills

Nizo, Ede, NL 10th April 2019 UR Grant writing/fund raising

Work Shop 5: Elucidating

Structure & Function in Biology

Newcastle University

24th-28th June 2019 UNew Advanced technical

research skills

Work Shop 6: Proteomics Newcastle University

24th-28th June 2019 UG & CSIC Advanced technical

research skills

Summer school 2:

Virulence & Stress Responses: Two Sides of the Same Coin?

Newcastle University

24th-28th June 2019 UmU (Aided

by NUIG, CSIC)

Understanding the latest advances in a research area.

Work Shop 7: Career

Planning for PhD Graduates

References

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