Transient expression of SPARC in the dorsal axis of early Xenopus embryos: correlation with calcium dependent adhesion and electrical coupling

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Figure

Fig. 1. Whole-mountin situ localizationof SPARCRNAduringearly Xenopusdevelopment.A 460 bp EcoRI fragmentof rhe 5' regionof XenopusSPARCwassubclonedmtopGEM-4Z,anddlgo\ogenin-Iabeledtranscnptsweregeneraredusingtheflankmgviral promotersandcognateRNApolymerase
Fig. 1. Whole-mountin situ localizationof SPARCRNAduringearly Xenopusdevelopment.A 460 bp EcoRI fragmentof rhe 5' regionof XenopusSPARCwassubclonedmtopGEM-4Z,anddlgo\ogenin-Iabeledtranscnptsweregeneraredusingtheflankmgviral promotersandcognateRNApolymerase p.3
Fig. 3. Mid-trunkcross-sectionalanalysisof SPARC RNA and proteinlocalization.To more closelye-.;aminethe distribution of SPARC RNA andprotein within the embryo, mid-trunk cross section were performed.IAI Atstage18 SPARCmessagewasfoundto be abundantin the n
Fig. 3. Mid-trunkcross-sectionalanalysisof SPARC RNA and proteinlocalization.To more closelye-.;aminethe distribution of SPARC RNA andprotein within the embryo, mid-trunk cross section were performed.IAI Atstage18 SPARCmessagewasfoundto be abundantin the n p.5

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