Microscope Notes 2014

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Parts of the Microscope and Their

Functions

Base Eyepiece Light Source Arm Body Tube Stage Stage Clips Coarse Adjustment Knob Diaphragm

(3)

Parts of the Microscope and Their

Functions

Eyepiece Light Source Arm Body Tube Stage Stage Clips Coarse Adjustment Knob Diaphragm

Revolving Nose piece Fine Adjustment knob Scanning Power

(4)

Parts of the Microscope and Their

Functions

Light Source Arm Body Tube Stage Stage Clips Coarse Adjustment Knob Diaphragm

Low Power High Power

(5)

Parts of the Microscope and Their

Functions

Arm Body Tube Stage Stage Clips Coarse Adjustment Knob Diaphragm

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Parts of the Microscope and Their

Functions

Body Tube Stage Stage Clips Coarse Adjustment Knob Diaphragm

Eyepiece

(7)

Parts of the Microscope and Their

Functions

Stage Stage Clips Coarse Adjustment Knob Diaphragm

(8)

Parts of the Microscope and Their

Functions

Stage Clips Coarse Adjustment Knob Diaphragm

(9)

Parts of the Microscope and Their

Functions

Coarse Adjustment Knob

Diaphragm

(10)

Parts of the Microscope and Their

Functions

Diaphragm

Revolving Nose piece Fine Adjustment knob Scanning Power Low Power High Power Eyepiece Arm Body Tube Stage

(11)

Parts of the Microscope and Their

Functions

Revolving Nose piece Fine Adjustment knob Scanning Power Low Power High Power Eyepiece Arm Body Tube Stage

Stage Clips Coarse Adjustment Knob

(12)

Parts of the Microscope and Their

Functions

Fine Adjustment knob Scanning Power Low Power High Power Eyepiece Arm Body Tube Stage

Diaphragm

(13)

Parts of the Microscope and Their

Functions

Scanning Power Low Power High Power Eyepiece Arm Body Tube Stage

Diaphragm

Revolving Nose piece

(14)

Parts of the Microscope and Their

Functions

Eyepiece Arm Body Tube Stage

Diaphragm

Fine Adjustment knob

Scanning Power

(15)

Parts of the Microscope and Their

Functions

Diaphragm

Scanning Power

High Power

(16)

Parts of the Microscope and Their

Functions

Diaphragm

Scanning Power

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Eyepiece

Arm Body Tube

Stage

Stage Clips Coarse Adjustment _Knob Diaphragm

Scanning Power Low Power

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Magnification

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Magnifying Lens

• Uses a single lens to magnify an object

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Microscopes

• Stereoscope

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Stereoscope

• Used to see larger

objects

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Compound Light Microscope

• Used for very small

specimens that light

can pass through

• Utilizes a glass slide

and coverslip

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Electron Microscope

http://school.discoveryeducation.com/lessonplans/interact/vemwindow.html

• Magnifies much smaller specimens

• Not for live cells or live specimens

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Appearance of Objects

• Inverted and reversed

(upside down and

backwards)

• Only a thin layer of the

specimen is in focus at

any level (depth of

focus)

• An “e” placed on the

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Movement of Objects

• 1. Actual movement is opposite to

appeared direction of movement.

• 2. If it appear to move it is

actually

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Total Magnification – Powers of

10

• Multiply ocular magnification times

objective magnification.

• Ocular = 10X

• Objectives =

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Focusing The Microscope

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Focusing The Microscope

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Focusing The Microscope

• Place Slide on stage

• Adjust diaphragm

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Focusing The Microscope

• Place Slide on stage

• Adjust diaphragm

• Turn revolving nosepiece to scanning power

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Focusing The Microscope

• Place Slide on stage

• Adjust diaphragm

• Turn revolving nosepiece to scanning power

(46)

Focusing The Microscope

• Place Slide on stage

• Adjust diaphragm

• Turn revolving nosepiece to scanning power

• Turn course adjustment knob until image comes into focus • Repeat These Steps for Low Power

• Use only the fine adjustment knob with high power

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