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An Irreversible Inhibitor of HSP72 that Unexpectedly Targets Lysine-56.

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Figure

Figure 2. HSP72-NBD Cys17 irreversible inhibitor model generatedusing MOE 2013.0801, only key residues are shown, solvent andhydrogens omitted for clarity, carbon=grey, oxygen=red, nitro-gen=blue, chlorine=green, sulfur-=yellow.
Figure 6. a) Trypsin-digest MS/MS indicating modification of HSP72-NBD with 8 at residue Cys267 (precursor ion 356.47563+, error�0.84 ppm) b) Proposed schematic of fragmentation of 8 upon MS/MS.
Figure 8. a) Extracted ion chromatogram of the MS1 from the trypsin digest of K56A HSP72-NBD with 8

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