Special, Immunohistochemistry (IHC), & Chromogenic in situ Hybridization (CISH) Stains

Special, Immunohistochemistry (IHC),

& Chromogenic

in situ

Hybridization (CISH) Stains

Special Stains

Group I for Microorganisms

Special Stains, Group I 88312

Fite

Mycobacteria leprae

PAS/Light Green Counterstain Fungus

Gomori’s Methenamine Silver (GMS) Fungus, Pneumocystis, Bacteria

Warthin-Starry Bacteria, Spirochetes Gram

Gram-Positive & Gram-Negative Bacteria

Ziehl-Neelsen Acid-Fast Bacilli (AFB) Acid-Fast Mycobacterium

Group II - All Other

Special Stains, Group II 88313

Alcian Blue, pH 2.5 Acid Mucopolysaccharides

Luxol Fast Blue Myelin

Alcian Blue/Hyaluronidase

Epithelial and Connective Tissue Mucin Differentiation

Masson Trichrome Collagen

Alcian Blue/Periodic Acid-Schiff (PAS) Acid and Neutral Mucins

Melanin Bleach Eliminates Melanin Bielschowsky

Neurofibrils

Movat

Collagen, Elastic Fibers, Fibrin, Mucin, Muscle Bile, Hall’s Method

Bilirubin Mucicarmine Mucosubstances Colloidal Iron Mucopolysaccharides, Collagen Oil Red O Fat Colloidal Iron/Hyaluronidase

Epithelial and Connective Tissue Mucin Differentiation

Periodic Acid-Schiff (PAS)

Glycogen, Neutral Mucopolysaccharides, Basement Membranes

Congo Red Amyloid

Periodic Acid-Schiff/Amylase (PAS/D) Eliminates Glycogen

Copper (Rhodanine) Copper

Phosphotungstic Acid Haematoxylin (PTAH)

Myelin Crystal Violet

Amyloid

Reticulum Reticulum Fibers Elastic Stain (EVG)

Elastic Fibers, Collagen

Sodium Sulphate-Alcian Blue (SAB) Amyloid

Fontana-Masson

Argentaffin Granules or Melanin

Thioflavin S Amyloid Giemsa (Mast Cell)

Eosinophilic Granules and Mast Cells

Toluidine Blue Mast Cell Granules Iron Stain

Iron

Uric Acid Urate Crystals Jones Methenamine Silver

Basement Membranes, Reticular Fibers

Von Kossa Calcium

Special Stains

Special, Immunohistochemistry (IHC),

& Chromogenic

in situ

Hybridization (CISH) Stains

Special Stains

Histochemical

Enzyme 88319

Stain name & primary demonstration

Acetylcholinesterase (ACE) Nerve Fibers/Hirschsprung’s Disease

Nicotinic Adenine Dinucleotide Dehydrogenase (NADH)

Whorls, Target Fibers, Central Cores, Moth-Eaten Fibers

Acid Phosphatase

Muscle Fibers undergoing Degeneration Alkaline Phosphatase

Basement Membranes, Regenerating Fibers

Phosphofructokinase (PFK) PFK Activity

Adenosine Triphosphatase (ATP): 4.6, 9.8 Muscle Fiber Types

Phosphorylase Phosphorylase Activity Cytochrome C Cytochrome Oxidase Succinic Dehydrogenase (SDH) Mitochondrial Disease

Immunohistochemistry (IHC) Stains

Human Adrenocorticotropic Hormone

Arginase 1 Normal Liver Cells

CA9

Carbonic Anhydrase IX Actin HHF35

Human Muscle Actin

ATRX Glioma

Calcitonin Calcitonin Actin SM

Alpha-Smooth Muscle Isoform, Myoepithelium, etc.

ß-Amyloid

Senile plaques, congofile deposits

Calponin

Myoepithelial Differentiation, Diagnosis of Myoepithelial Neoplasms Calreticulin

Expressed in Megakaryocytes when Calreticulin is mutated Adenovirus Adenovirus ß-Catenin Beta Catenin AFP Alpha Fetoprotein BAP1

Marker for differentiating Mesothelioma from Reactive Mesothelial Proliferations ALK (D5F3)

Anaplastic Lymphoma Kinase, Clone D5F3

BCL2 (124)

Follicular Lymphoma, Apoptosis Inhibin Protein, Clone 124

Calretinin

Calcium-Binding Protein, Mesothelial Cells ALK-1

ALK-1

BCL2 EP36 BCL2, Clone EP36

CAMTA1

Epithelioid Hemangioendothelioma Marker AMACR (p504s)

Prostate Cancer Marker

BCL-6

Burkitt’s Lymphoma,

Follicular and Diffuse Lymphomas, Lymphocyte-Predominant Hodgkin’s Disease

Cathepsin K Renal Angiomyolipoma Amyloid Kappa

Amyloidosis

C3d (Complement 3d) Transplant Rejection Biomarker Amyloid Lambda

Amyloidosis

B72.3 (TAG-72)

Tumor-Associated Glycoprotein

C4d (Complement 4d) Transplant Rejection Biomarker Androgen Receptor

Androgen Receptor

BOB-1

Transcription Coactivator for Oct-1 and Oct-2 CD1a

Cortical Thymocytes-Langerhans Cells Annexin A1

Hairy Cell Leukemia

BRAF V600E Hairy Cell Leukemia

CD2

Lymphocyte Function-Associated Antigen 2

Special, Immunohistochemistry (IHC),

& Chromogenic

in situ

Hybridization (CISH) Stains

Immunohistochemistry (IHC) Stains

T-Cells

CD31

Endothelium (Tumor Angiogenesis)

CD138

Plasma Cell Marker, Syndecan CD4 T-Cells, Helper T CD33 Monocytes CD163 Ber-MAC3 CD5

Pan T-Cells, B-Cell Subset, Thymic Carcinomas

CD34

Endothelium, Stem Cells, Stromal Tumors

CD200 by IHC

Chronic Lymphocytic Leukemia Marker

CD7

Thymocytes, Immature T-Cells, NK Cells

CD35 Dendritic Cells CDX-2 Metastatic Colon CA CD8 T-Cells, Suppressor T CD43

T-Cells, B-Cell Subset, Myeloid, Histiocytes, Plasma Cells (Leukosialin)

CEA (EPCEAR7 Monoclonal) Carcinoembryonic Antigen CD10

Common Acute Lymphoblastic Leukemia

CD44 Phagocytic Glycogen-1 Chromogranin Chromogranin A CD14 Monocytes, Histiocytes CD45

Helper, Inducer T-Cells

Chymotrypsin

Expressed in Cells of Pantcreatic Acinar Origin CD15 (BRA4F1)

BRA4F1

CD56

Natural Killer Cells

CL-NOTCH1 Lymphoma marker CD15 (MMA)

Epithelial, Hodgkin’s, Myeloid

CD57

Neural & Neuroendocrine Marker NK Cells, Stromal Tumors

Clusterin Apoptosis CD19 B-Lymphocyte Antigen CD61 GP111A c-MYC Lymphoma Marker CD20

Human B-Lymphocyte Antigen

CD68 (KP1)

Monocytes / Macrophages

CMV

Cytomegalovirus CD21

Dendritic Cells, B-Cells

CD68 (PG-M1) Sarcomas, Leukemia CXCL-13 B-Lymphocyte Chemoattractant CD22 Mature B-Cells CD71

Erythroid Precursor Marker

Cyclin-D1

Cyclin D1/PRAD-1 Oncogene Product, Mantle Cell

CD23

Activated B-Cells

CD79a B-Cells

Cytokeratin AE1 / AE3 Keratin Cocktail:

40, 48, 50, 50, 6, 58, 65, 5 kD CD25

Activated T-Cells & B-Cells

CD99

Ewing’s Sarcoma / PNET

Cytokeratin CAM5.2 Keratin: 39, 43, 500 kD CD30 (BER-H2)

Activated Band T-cells

CD117 C-Kit

Cytokeratin 5/6

Intermediate Filament Protein CD30 (JCM182)

Activated Band T-cells

CD123

Alpha Receptor for IL3

Cytokeratin 7 Keratin: 54 kD

Special, Immunohistochemistry (IHC),

& Chromogenic

in situ

Hybridization (CISH) Stains

Immunohistochemistry (IHC) Stains

Human Cytokeratin 19 Polypeptide

Fascin

Hodgkin’s Disease, High-Grade Breast Carcinoma

Granzyme-B Serine Protease Cytokeratin 20 Keratin: 54 kD FCRL4 (IRTA1) Lymphoma marker H3.3G34W, Mutant

Marker for Giant Cell Tumor of Bone Cytokeratin KER903 (34BE12)

High-Molecular Weight Cytokeratins

FGFR3

CD333, Fibroblast Growth Factor Receptor 3

H3K27me3, Wild

Malignant Peripheral Nerve Sheath Tumor Marker Cytokeratin OSCAR Cytokeratins 7, 8, 18, 19 FOSB Pseudomyogenic Hemangioepithelioma H3K27M, Mutant Glioma/Astrocytoma D2-40

Podoplanin, Lymphatic Endothelial Marker

FOXL2

Ovarian Sex Cord-Stromal Tumors (SCSST)

HBcAg

Hepatitis B Core Antigen Desmin

Desmin

FSH

Follicle-Stimulating Hormone

HBsAg

Hepatitis B Surface Antigen DOG-1

Gastrointestinal Stromal Tumor (GIST) Marker

Fumarate Hydratase

Hereditary Leiomyoma, Renal Cell Carcinoma

H-Caldesmon

Smooth Muscle Differentiation DUX4

CIC-DUX4 Sarcoma from Ewing Sarcoma

Gastrin

Gastrin-Secreting Tumors, G-Cell Hyperplasia

H.pylori

Helicobacter pylori

EBNA-2

Stains Nuclei of Epstein-Barr Virus (EBV)-Infected Lymphoblastoid Cells

GATA-3

Urothelial Carcinoma Marker

HCG

Human Chorionic Gonadotropin (HCG) in Trophoblastic Elements of Germ Cell Tumors

EBV

Epstein-Barr Virus

GCET1

Germinal Center B-Cell Marker

Hemoglobin-A Hemoglobin-A E-cadherin

Epithelial Marker, EP-CAM

GFAP

Human Glial Fibrillary Acidic Protein

Hepatocyte Hep-par1 EMA

Epithelial Membrane Antigen

GFAP/OLIG2 Double Stain Oligodendroglioma vs. astrocytomas Herpes HSV I & II Enolase (NSE) Neuron-Specific Enolase GH Growth Hormone HER2

Erb-2 Oncoprotein, Clone 4B5 EP4-Ber

Epithelial Antigen, EP-CAM

GLUT1

Glucose Transporter 1

HHV8

Human Herpes Virus 8 ER

Estrogen Receptor

Glutamine Synthetase Hepatocellular Carcinoma Marker

HMB-45

Melanoma-Associated Marker ERG

Vascular, Prostate Malignancy Marker

Glycophorin A

Identification of Cells of Erythroid Lineage HPV

Human Papilloma Virus Factor XIIIa

Fibrohistiocytic Tumors, Dendritic Interstitial Cells

Glypican-3

Hepatocellular Carcinoma Marker

ICOS

Inducible Co-Stimulate CD278

Special, Immunohistochemistry (IHC),

& Chromogenic

in situ

Hybridization (CISH) Stains

Immunohistochemistry (IHC) Stains

Glioma Marker

LMO2

Large B-Cell Lymphoma Marker

Myogenin

Rhabdomyosarcoma Marker IgA

Immunoglobulin A

MAL

Myelin and Lymphocyte Protein expressed in Primary Mediastinal Large B-Cell Lymphoma

Napsin A

Lung Adenocarcinoma Marker IgD

Immunoglobulin D

Mammaglobin

Primary and Metastatic Breast Carcinoma Marker

NeuN

Neuronal Cell Loss in Drug-Resistant Epilepsy IgG Immunoglobulin G MDM2 Liposarcoma Neurofilament (2F11) Neurofilament IgG4 Autoimmune Pancreatitis MEF2B

B cell lymphoma marker

Neurofilament Cocktail

Cocktail of Clones 2F11 and SMI-32 IgM

Immunoglobulin M

Melan A

Malignant Melanoma Marker

NKI/C3

Melanoma Marker, CD63 Inhibin

Granulosa Cell Tumor, Sex Cord Stromal Tumor

MiTF

Melanocyte Development Regulator, Melanoma Oncogene

NKX3.1

Differential Marker for Prostate vs. Urothelial Carcinoma INI1

Malignant Rhabdoid Tumor Marker

MLH-1

Mismatch Repair Gene MLH-1

NUT

Nuclear Protein in Testis INSM1

Neuroendocrine marker

MOC-31

Differentiates Carcinomas from Mesothelial Cells and Mesotheliomas

OCT-2

Octamer Transcription Factor 2 Kappa

Kappa Light Chain

MSH-2

Mismatch Repair Gene MSH-2

OCT3/4

Marker for Undifferentiated Cells Ki67

Cell Proliferation Marker

MSH-6

Mismatch Repair Gene MSH-6

OLIG2 211F1-1 Ki67-Melan A Double Stain

Proliferative Cells and Melanocytes

MUC4

Fibromyxoid Sarcoma

p16

Cervical Cancer Marker Lambda

Lambda Light Chain

MUM-1

Multiple Myeloma Oncogene-1

p40

Pulmonary Squamous Cell Carcinoma Marker Langerin

Langerhans Cell Histiocytosis

Muramidase Muramidase/Lysozyme

p53

Over-Expression of the p53 Oncogene LEF1

Chronic Lymphocytic Leukemia (CLL), Small Lymphocytic Leukemia (SLL)

Myeloperoxidase

Neutrophilic Granulocytes and Monocytes p57

Differential Diagnosis of Complete vs. Partial Hydatidiform Molar Pregnancy LH

Luteinizing Hormone

MyoD1

Rhabdomyosarcoma Marker

p63

Prostate Cancer Marker

Special, Immunohistochemistry (IHC),

& Chromogenic

in situ

Hybridization (CISH) Stains

Immunohistochemistry (IHC) Stains

Double Stain for Prostate Cancer

pSTAT 5

Surrogate Marker of JAK2 V617F Mutation TAU

Neurofibrillary Tangles P. jiroveci

Detection of Pneumocystis jirovecii

PTH

Parathyroid Hormone

Tau/ß-Amyloid DS

Neurofibrillary Tangle and Amyloid in Alzheimer’s Postmortem Specimens PAX-2

Breast, Kidney, Ovary, Prostrate Tumor Marker

S-100 S100 protein

TCL-1A

Mature T-Cell Leukemia PAX-5

B-Cell-Specific Activator Protein

SALL4

Yolk Sac Tumor Marker

TCR ß F1 T-Cells PAX-8

Nephritic Cell Lineage Marker

SATB2 GI Epithelial Marker TCR-Delta T-Cells PD-L1 Clone 22C3 (LDT) SDHA

Succinate Dehydrogenase Subunit A

TdT

Blasts, Immature Lymphocytes PD-L1

Clone SP142 (IVD)

SDHB

Succinate Dehydrogenase Subunit B

Thyroglobulin Thyroglobulin Perforin

T-Cell and NK-Cell Lymphoma

SF-1

Adrenocortical Tumor Marker

TIA1

Cytolytic Effector Cells in Lymphocytic Infiltrates PIN Cocktail (p63/AMACR)

p63 and AMACR

SMARCA4 (BRG1)

Deficient in several uncommon, aggressive tumors

TLE-1 (1F5)

Synovial Sarcoma Marker PLAP

Placental Alkaline Phosphatase, Germ Cell, Adenocarcinoma

SMMS1

Smooth Muscle Myosin

Transthyretin Amyloidosis, Prealbumin PMS2

Mismatch Repair Gene PMS-2

SOX-10

Identifies Melanomas, Nerve Sheath Tumors

Tryptase, Mast Cell Human Mast Cells PR

Progesterone Receptor

SOX-11

Mantle Cell Lymphoma Marker

TSH

Thyrotropic Cells of Pituitary PRAME

Differentiates Melanoma vs. Nevus

SSTR2A

Meningioma vs. Schwannoma

TTF-1

Thyroid, Pulmonary Carcinomas Prolactin

Prolactin in Pituitary

STAT6

Solitary Fibrous Tumor

Tyrosinase Metastatic Melanoma PSA

Prostate-Specific Antigen

SV40

Simian Virus 40, Polyomavirus Family

Vimentin Vimentin PSAP

Normal, Hyperplastic, Neoplastic Prostatic Cells

Synaptophysin Synaptophysin

VS38C Plasma Cell PSMA

Prostate-Specific Membrane Antigen

Syphilis

Treponema pallidum

WT1 (6F-H2) Wilms tumor

Cleveland Clinic Laboratories

Special, Immunohistochemistry (IHC),

& Chromogenic

in situ

Hybridization (CISH) Stains

Chromogenic

in situ

Hybridization (CISH)

CISH EBER

Epstein-Barr Virus (EBV)

CISH Kappa Kappa

CISH Lambda Lambda

CISH HPV – Low Risk (LR6)

Human Papilloma Virus, 6 Low-Risk Subtypes: 6, 11, 40, 42, 43, 44

CISH HPV – High Risk (HR18)

Human Papilloma Virus, 18 High-Risk Subtypes: 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 73, 82