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The nuclear receptor ERβ engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading

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Figure

Fig. 1 Effects of unliganded ERthreshold.Ct-ERdifferentially expressed in BC cells expressing either Ct-ERinclusion/exclusion cut-off |0.1|).β on the BC cell transcriptome and alternative RNA splicing
Fig. 2 Unliganded ERER7 cells (most enriched within ERcell genome. The heatmap shows read density in the 10-kb regions centered on each binding site in Ct-ERβ binding site identification and annotation in BC cells
Fig. 3 ERβ-binding proteins identified by interaction proteomics. a The experimental workflow
Fig. 4 Validation of ERcontent are reported. ESR2 (bait) and AGO2 are highlighted inmeasured in three biological replicates.AGO1 co-immunoprecipitation with ERand EIF6 from nuclear extracts of a Tet-inducible MCF-7 cell clone expressing Myc-Flag-ERvalue no
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