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Systematic evaluation of CRISPR Cas systems reveals design principles for genome editing in human cells

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Figure

Fig. 1 Evaluation of various CRISPR-Cas systems in NHEJ-mediated genome editing using perfectly matched spacers or spacers with overlappingseeds
Fig. 2 Relationship of DNA cleavage efficiency with gene expression and target specificity.test)
Fig. 3 Evaluation of various CRISPR-Cas systems in HDR-mediated genome editing using symmetric ssODN donor templates and spacers withTheSpCas9 were comparable to those of AsCpf1 and LbCpf1, as determined by the T7E1 assays
Figure S19). Since the six additional sites are located ingenes of varying expression levels, the higher HDR effi-ology [ciency exhibited by Cpf1 appears to be independent ofthe underlying chromatin architecture
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