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Accurate Detection of Methicillin Resistant Staphylococcus aureus in Mixtures by Use of Single Bacterium Duplex Droplet Digital PCR

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Figure

FIG 1 Two-dimensional (2D) plots (A), calibration curves (B), and the average MRSA index ratio (MIR) of MRSA (C) of duplex ddPCR for detection of MRSA N315per ml, and thefluorescence amplitude corresponding to the HEX fluorophore (fluorophore (positive; green
FIG 2 The average MRSA index ratios (MIRs) (A) and two-dimensional (2D) plots (B) of duplex ddPCR for detection of the mixture of MSSA 91118 and MR-CoNSWH01 under different concentrations (a, 1 � 106 CFU/ml each; b, 1 � 105 CFU/ml each; c, 1 � 104CFU/ml ea
TABLE 2 Sensitivity and specificity of the qPCR and ddPCR methods for detection ofMRSA in 104 nasal specimens
FIG 3 PCR amplicons of theN315 and MSSA strain 91118 were used as the controls. Lanes 38, 39, and 40 were isolated from specimen 38; lanes 41, 42, and 43 were isolated from specimen MREJ region (A) using the primer pair rjmec and ORFX1r and mecA (B) for co

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