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Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007

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Figure

Figure 1 Camphor degradation in Pseudomonas putida NCIMB 10007: In the first step camphor (1) is hydroxylated by the P450 Cam - -monooxygenase (Unger et al
Figure 3 Vector 2,5-DKCMO_pET-28 for expression of recombinant 2,5-DKCMO from P. putida NCIMB 10007 under control of T7 promoter in E
Figure 4 SDS-PAGE analysis of 2,5-DKCMO: lane 1: marker: 150, 130, 100, 70, 55, 35, 25, 15 kDa; lane 2: crude extract (41 μg total protein); lane 3: purified protein (22 μg).
Table 1 Purification of 2,5-DKCMO via nickel-based affinity chromatography and imidazole removal

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