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An efficient in vitro propagation protocol for snowdrop anemone Anemone sylvestris L.)

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Fig. 1. Plant regeneration of snowdrop anemone. (A) bulking of stock material on PGR-free medium (left AS2 clone, right AS4 clone); (B) adventitious shoot regeneration on shoot induction media (MS medium with 0 or 1 µM BA, zeatin or kinetin) of the AS4 clone after three weeks; (C) detail of malformed leaves of the AS4 clone grown on medium with 1 µM BA; (D) adventitious shoot regeneration on shoot induction media (MS medium with 0 or 1 µM BA, zeatin or kinetin) in the AS2 clone after three weeks; (E) rooted shoot of the AS4 clone on PGR-free medium after three weeks; (F) callus induction in an unrooted shoot of the AS4 clone on medium supplemented with NAA after three weeks; (G) rooted shoot of the AS4 clone on medium supplemented with NAA and AC after three weeks; (H) acclimatisation of rooted plantlets in the culture room; (I) young plants in the cold frame
Table 1. Effect of cytokinins (BA, kinetin and zeatin) on adventitious shoot regeneration of two snowdrop anemone clones (AS2 and AS4) after three weeks in culture
Table 2. Effect of different factors (genotype and medium composition) on shoot regeneration of snowdrop anemone
Table 4. Effect of growth regulators (IBA, NAA and BA) and AC on root formation in shoots of snowdrop anemone (AS2 and AS4 clones) after three weeks in culture
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