Preamble. 1. If it is necessary, are you willing to receive follow up questions to help clarify any answers to the survey questions?

The purpose of this inter­organizational survey is to gather data regarding transplant center practices for preparing cord 

blood units for administration, including characterizing and confirming the identity of cord blood units and thawing, 

diluting, and washing the units. 

 

You will be asked 34 questions; some questions relate to four different scenarios regarding cord blood units. After using 

this version to compile answers to the questions, submit your responses online at 

https://www.surveymonkey.com/s/cbupractices. The time to complete the survey is estimated to be about 20 minutes 

after compiling all information for your transplant center.  

1. If it is necessary, are you willing to receive follow up questions to help clarify any

answers to the survey questions?

Preamble

 

Yes  n m l k j No, I wish to remain anonymous.  n m l k j

2. Name (optional)

 

3. Email (optional)

 

4. Region of the World

5. Transplant Center Size by Number of Cord Blood Transplants per Year (Adult and

Pediatric)

6. Does your center consider non­inherited maternal antigens (NIMA) in cord selection

when the unit is not a 6/6 match?

Contact Information (Optional)

 

North America  n m l k j Europe  n m l k j Asia  n m l k j Australia/New Zealand  n m l k j Central America/South America  n m l k j Africa  n m l k j 0­10  n m l k j 11­20  n m l k j 21­30  n m l k j 31­50  n m l k j < 50  n m l k j Always  n m l k j Most of the time  n m l k j Occasionally  n m l k j Never  n m l k j Comment  5 5 6 6

7. Please indicate below all standard testing performed AT YOUR CENTER after receipt of

the ENTIRE cord blood unit (CBU) and prior to administration. (Check all that apply.)

8. If viability is performed, what method is used?

Testing of the Cord Blood Unit After Receipt and Prior to Infusion

Sample taken prior to infusion Results required prior to infusion TNC gfedc gfedc WBC Differential gfedc gfedc CD34 enumeration gfedc gfedc Viability (any method) gfedc gfedc ABO typing (with or without  Rh) g f e d c gfedc Platelet count gfedc gfedc Hematocrit/Hemoglobin gfedc gfedc HLA gfedc gfedc HLA antibody gfedc gfedc CFU gfedc gfedc None _{gfedc gfedc}

 

Other (please specify)  Trypan blue  g f e d c 7­AAD  g f e d c We do not perform viability  g f e d c Other  5 5 6 6

For questions 9­12, assume that CBU confirmatory typing was performed via the registry or cord blood bank on an 

attached segment prior to shipment to your center. 

9. Which tests are performed after receipt and prior to administration to confirm identity of

the CBU? (Select all that apply.)

10. For the identity tests described in question 9, what source of CBU material does your

center prefer to use (assume all types of materials are available for the CBU)?

11. For pre­administration HLA identity testing, please check all standard loci tested:

12. For pre­administration HLA identity testing, please indicate the typing methodology:

Scenario 1

ABO typing (with or without Rh)  g f e d c HLA  g f e d c None required prior to administration for this scenario  g f e d c Other (please specify)  Attached segment material only  n m l k j Any material (ancillary or attached) associated with the cord blood unit  n m l k j No identity testing performed at my center is required prior to administration for this scenario  n m l k j HLA­A  g f e d c HLA­B  g f e d c HLA­C  g f e d c HLA­DRB1  g f e d c HLA­DQB1  g f e d c HLA­DPB1  g f e d c No HLA identity testing performed prior to infusion for this scenario  g f e d c Serology for all classes of loci  n m l k j Serology for Class I loci, DNA­based for Class II loci  n m l k j DNA­based for all classes of loci  n m l k j RT­PCR  n m l k j No HLA identity testing done prior to administration for this scenario  n m l k j

For questions 13­16, assume that CB confirmatory typing was performed on ancillary material but not on an attached 

segment prior to shipment to your center and an attached segment is available. 

13. Which tests are performed after receipt and prior to administration to confirm identity of

the CBU? (Select all that apply.)

14. For the identity tests described in question 13, what source of CBU material does your

center prefer to use (assume all types of materials are available for the CBU)?

15. For pre­administration HLA identity testing, please check all standard loci tested:

16. For pre­administration HLA identity testing, please indicate the typing methodology:

Scenario 2

 

ABO typing (with or without Rh)  g f e d c HLA  g f e d c None required prior to administration for this scenario  g f e d c Other (please specify)  Attached segment material only  n m l k j Any material (ancillary or attached) associated with the cord blood unit  n m l k j No identity testing performed at my center is required prior to administration for this scenario  n m l k j HLA­A  g f e d c HLA­B  g f e d c HLA­C  g f e d c HLA­DRB1  g f e d c HLA­DQB1  g f e d c HLA­DPB1  g f e d c No HLA identity testing performed prior to administration for this scenario  g f e d c Serology for all classes of loci  n m l k j Serology for Class I loci, DNA­based for Class II loci  n m l k j DNA­based for all classes of loci  n m l k j RT­PCR  n m l k j No HLA identity testing done prior to administration for this scenario  n m l k j

For questions 17­22, assume that extended typing was performed on ancillary material but not on an attached segment 

prior to shipment. Ancillary material is available but NO attached segment is available. 

17. If this CBU was the best HLA match and dose for a patient, would your center accept

it?

Scenario 3

 

Yes  n m l k j No  n m l k j

18. Which tests are performed after receipt and prior to administration to confirm identity of

the CBU? (Select all that apply.)

19. For the identity tests described in question 18, what source of CBU material does your

center prefer to use (assume all types of materials are available for the CBU)?

20. For pre­administration HLA identity testing, please check all standard loci tested:

21. For pre­administration HLA identity testing, please indicate the typing methodology:

Scenario 3

ABO typing (with or without Rh)  g f e d c HLA  g f e d c None required prior to administration for this scenario  g f e d c Other (please specify)  Aliquot of thawed CBU  n m l k j Any material associated with the cord blood unit  n m l k j No identity testing performed at my center is required prior to administration for this scenario  n m l k j HLA­A  g f e d c HLA­B  g f e d c HLA­C  g f e d c HLA­DRB1  g f e d c HLA­DQB1  g f e d c HLA­DPB1  g f e d c No HLA identity testing performed at my center prior to administration for this scenario  g f e d c Serology for all classes of loci  n m l k j Serology for Class I loci, DNA­based for Class II loci  n m l k j DNA­based for all classes of loci  n m l k j RT­PCR  n m l k j No HLA identity testing performed at my center prior to administration for this scenario  n m l k j

22. Which tests are performed after administration to confirm identity of the CBU? (Select

all that apply.)

 

ABO typing only (with or without Rh)  g f e d c HLA typing only  g f e d c Both HLA and ABO typing  g f e d c Post­administration identity testing not performed in this scenario  g f e d c

For questions 23­28, assume that extended typing was performed on ancillary material but not on an attached segment 

prior to shipment; no further material is available. 

23. If this CBU was the best HLA match and dose for a patient, would your center accept

it?

Scenario 4

 

Yes  n m l k j No  n m l k j

24. Are any tests performed after receipt and prior to administration to confirm identity of

the CBU in this scenario?

25. Which tests are performed after receipt and prior to administration to confirm identity of

the CBU? (Select all that apply.)

26. For pre­administration HLA identity testing, please check all standard loci tested:

27. For pre­administration HLA identity testing, please indicate the typing methodology:

Scenario 4

Yes, an aliquot of the thawed CBU would be used  n m l k j Not for this scenario  n m l k j Other (please specify)  ABO typing (with or without Rh)  g f e d c HLA  g f e d c None required prior to administration for this scenario  g f e d c Other (please specify)  HLA­A  g f e d c HLA­B  g f e d c HLA­C  g f e d c HLA­DRB1  g f e d c HLA­DQB1  g f e d c HLA­DPB1  g f e d c No HLA identity testing performed prior to administration for this scenario  g f e d c Serology for all classes of loci  n m l k j Serology for Class I loci, DNA­based for Class II loci  n m l k j DNA­based for all classes of loci  n m l k j RT­PCR  n m l k j No HLA identity testing done prior to administration for this scenario  n m l k j

28. Which tests are performed after administration to confirm identity of the CBU? (Select

all that apply.)

 

ABO typing only (with or without Rh)  g f e d c HLA typing only  g f e d c Both HLA and ABO typing  g f e d c Post­administration identity testing not performed in this scenario  g f e d c

29. When does your center perform post­administration identity testing (HLA and/or ABO)?

30. When post­administration identity testing is performed, please indicate which type of

testing is performed:

Post­Administration Testing

 

For all CBUs  n m l k j When identity could not be performed pre­administration via an attached segment or bag wash  n m l k j When identity could not be confirmed pre­administration via ancillary material  n m l k j Never  n m l k j Other (please specify)  ABO only (with or without Rh)  n m l k j HLA by serology only  n m l k j HLA by DNA methods with or without serology  n m l k j HLA with RT­PCR  n m l k j ABO and/or HLA  n m l k j Post­administration identity testing is not performed  n m l k j

31. When do you thaw CBUs?

32. For double cord blood transplants, do you wait to thaw the second unit until after the

first unit has been administered?

33. In the table below, please indicate the preparative method(s) used at your center for a

given type of cord blood unit.

34. What is your washing and/or dilution method?

 

Thawing, Washing, and Dilution

Thaw and Infuse ­ No Processing Thaw and Dilute (for example,  Albumin/Dextran) Thaw and Wash Red Cell Replete Cord  Blood Unit g f e d c gfedc gfedc Dextran­Containing Cord  Blood Unit g f e d c gfedc gfedc RBC Deplete Cord Blood  Unit ­ Small Volume (less  than 30 mL) g f e d c gfedc gfedc RBC Deplete Cord Blood  Unit ­ Large Volume  (greater than or equal to 30  mL) g f e d c gfedc gfedc 5 5 6 6 Prior to administration in the processing facility  n m l k j At the time of infusion at the bedside  n m l k j Either, depending on the circumstances  n m l k j Always  n m l k j Most of the time  n m l k j Occasionally  n m l k j Never  n m l k j We do not perform double cord blood transplants.  n m l k j