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Modulation of limb bud chondrogenesis by retinoic acid and retinoic acid receptors

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Figure

Fig. 3. Relative levels of syn-thesis of procollagen type IIA
Fig. 4. Effectof antisenseformspM)oligonucleotides(AS) for individualiso.of RAAs(a, p
Fig. 6. Microscopyofsectionedcultures.AzuremicromassstainedwithII.(AIContra/'unsupplementedcul-ture
Fig. 9. AS-p2doesJ.lM) alone or in the presenceperiod.producedtionsion in proliferationcell multiplicationnot changecell prolifera-ratein controlor RA-treatedcultures.(AI 3H-thymidineincorporation.The cells wereexposedto 3H-thymidinefor 5 h beforehar-vestingon each day of culture.Each pointrep-resentsmean (z5D) of 4 micromass cultures.Proliferationrate peakson day 3 in controlcul-tures and then declines.RA inducesa depres-rate throughoutthe culture(81 Cell numberat the end of 4 day cul-ture period.The additionof A5-fJ2 (6 }1M) aloneno significantchangewhileRA (0.3of A5-fJ2 reducedInmicromasscultures.

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