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Characterization of nonstructural protein 3 of a neurovirulent Japanese encephalitis virus strain isolated from a pig

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Figure

Figure 1 Cloning and expression of the NS3 gene. (A) NS3 gene was amplified from JEV-infected cells by RT-PCR and checked by agarosegel electrophoresis
Figure 2 Analysis of the specificity of the anti-NS3 antibody produced. (A) Western blot analysis of Flag-NS3 expressed in Neuro-2a cells.Cell lysates (20 μg) from Neuro-2a cells transfected with plasmid expressing Flag-NS3 were probed with the anti-NS3 an
Figure 3 Detection of the expression of NS3 by western blot analysisimmunoblotted with the anti-NS3 antibody and re-probed with anti-SH-JEV01 strain of JEV at MOI 5 and analyzed 12 h post-infection (hpi)
Figure 4 Analysis of the intracellular localization of NS3. Neuro-2a (A) and PIEC cells (B) were infected with the SH-JEV01 strain of JEV andimmunostained with anti-NS3 antibody (red) at the indicated times
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