2 0 0 5 – 2 0 0 6 P R O D U C T C ATA LO G
TrueClone:
Dear Valued Customer,
The completion of the sequencing of the human genome provides us with an enormous amount of data
related to individual genes and their transcripts. There remains, however, a large informational gap between
a gene’s sequence and its biological function, and an even greater gap between the gene’s function and its
complicated interactions with other genes. Unraveling and understanding gene functions and gene networks
will undoubtedly be a great challenge for some time to come.
In 1997, OriGene formulated a vision that the availability of a physical collection of all human genes will be
required to dissect gene function and genetic interactions. After over eight years of dedication, OriGene scientists
have isolated more than 24,000 distinct human full-length cDNA clones representing over 15,000 loci, all in a
unified pCMV expression ready vector. We have built the world’s largest searchable collection of cDNA clones,
the TrueClone Collection
TM. We are proud of the fact that thousands of innovative researchers worldwide have
been taking advantage of these ready-made clones to accelerate their research.
The value of the TrueClone collection is multifaceted:
1.
As a source of individual genes
. When an authentic human full-length cDNA clone is readily available,
scientists are alleviated of the tedious work of gene cloning and can better direct their time and resources
to more exciting discoveries downstream.
2.
As a collection of gene families
. For laboratories with relatively focused interests, the TrueClone collection
can be used as an inventory for scientists to pick-and-assemble their own sub-collections and conduct
functional assays. OriGene has pre-assembled several such sub-collections based on biological pathways
or gene families, including protein kinases, GPCR’s, nuclear hormone receptors, and cytochrome
P-450 genes.
3.
As a comprehensive collection
. Expression-ready TrueClones provide unprecedented opportunities for
scientists to functionally test thousands of human genes in a uniformed cell based assay. This approach,
which we call FlagArray (Full-Length cDNA Annotation of Gene functions), was recently employed by
scientists at the Genomics Institute of the Novartis Research Foundation. Using 20,000 cDNA clones,
including a large number of OriGene TrueClones, they successfully discovered and characterized several
novel modulators of the Wnt signaling pathway.
1OriGene is dedicated to its mission of isolating every single human full-length cDNA clone that represents a
functional transcript in the human genome. We are determined to make TrueClone the ultimate collection: where
every gene can be found, and that every scientist can trust. We understand this is a formidable task. To adhere to
the highest quality standards when isolating authentic clones is costly and time consuming; yet OriGene is
committed to this effort. This strong commitment, coupled with our diverse scientific expertise, has made the
TrueClone collection not only the most comprehensive available, but also the highest quality source. We welcome
all suggestions from our customers to 1) enhance our gene collection 2) provide more extensive downstream
applications and 3) improve our product support. We are especially interested in developing innovative
technologies that functionally assay the human genome in its entirety. If you have an idea, an invention or a
research program potentially involving large subsets of our collection of human full-length cDNA clones, please
do not hesitate to contact us. The perfect collection of human full-length cDNA clones can only be attained via input
and feedback from scientists like you. We look forward to earning your trust and to being your discovery partner.
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Sections
I.
TrueClone™—Ready-cloned non-PCR human
full-length cDNA in CMV vectors ...1
Human full-length genes...2
CloneSet: Gene-family based collection ...10
• Kinase CloneSet
• GPCR CloneSet
• Nuclear Hormone Receptor CloneSet
• Ion Channel CloneSet
• P450 CloneSet
II. HuSH™ ...45
Premade shRNA expression panels ...46
pRS vectors...61
III. Full-length cDNA cloning...63
Rapid-Screen™ arrayed cDNA libraries ...64
• Human cDNA Libraries
• Mouse cDNA Libraries
• Rat cDNA Libraries
Rapid-Screen™ Longest-Clone
SMservices ...72
• Human 12-Tissue Library Screen
• Mouse 6-Tissue Library Screen
Sure-RACE™ 5’-RACE Kit ...75
• Human 12-Tissue cDNA
• Mouse 12-Tissue cDNA
Yeast two-hybrid expression cDNA libraries ...81
• DupLEX-A™ yeast two-hybrid system kit
• Human Libraries
• Mouse Libraries
• Rat Libraries
• Breast Cancer Libraries
• Prostate Cancer Libraries
• Other Libraries
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IV. Gene expression ...85
Rapid-Scan™: PCR-based gene expression profiling panel...86
• Human Tissue Panel (24 Tissues)
• Human Brain Panel (12 Parts)
• Human Breast Cancer (24 Normals/Tumors)
• Mouse Tissue Panel (24 Tissues)
• Mouse Brain Panel (48 Parts/Stages)
• Drosophila Tissue Panel (12 Parts/Stages)
Real-Time-Scan™: Real-Time PCR-based gene expression profiling panel ...86
• Human Tissue Panel: (48 tissues) NEW
Northern Blot...95
• Human 12-Tissue RNA Blot I
• Human 12-Tissue RNA Blot II
• Human 6-tissue RNA Blot III
• Mouse 12-Tissue RNA Blot
• Rat 12-Tissue RNA Blot
• Rat 12 Brain-Part RNA Blot
Multiple-Choice™ cDNAs ...101
• Human Tissue cDNA
• Mouse Tissue cDNA
• Rat Tissue cDNA
Blue-Ribbon™ Poly A
+and Total RNAs...105
• Human Tissue RNA
• Mouse Tissue RNA
• Rat Tissue RNA
V. Miscellaneous molecular tools...109
Pre-validated antibodies ...110
• Kinase antibodies
• GPCR antibodies
Protein collections ...130
Quanti-Ladder ... 140
Broad range DNA size marker (200bp-10kb) with quantity reference
Millennium™ marker ...141
Broad range RNA size marker (0.5kb-9kb)
Rapid-Load...142
Innovative PCR compatible loading buffer
TrueClone™
Ready-cloned non-PCR human full-length cDNA in CMV vectors
Over 24,000 individual clones
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Ready-cloned non-PCR human full-length
cDNA in CMV vectors
All TrueClones are fully searchable using the search
engine on our website. Each clone is represented by
a unique catalog number of 6 digits with a prefix of
“TC”.
OriGene’s TrueClone Collection contains over 24,000
full-length cDNA clones whose nucleotide sequences
can be found in the public domain. They represent
about 85 percent of the transcript sequences that
have been annotated by NCBI.
OriGene’s mission is to clone every single human
full-length transcript. Our cloning effort is aimed at
expanding TrueClone coverage until our mission is
accomplished. Please visit our website often for the
most updated status. If your gene of interest is not
yet included in our collection, you are encouraged to
fill out a cloning request form and our scientists will
make every effort to clone your gene for you.
ADVANTAGES
The most comprehension collection:
over 24,000 (and growing)
cDNA clones available
Authentic clones: no PCR artifacts
Expression-Ready: All clones are
expression-optimized in a CMV vector
Quick delivery: Most clones will be
shipped within 48 hrs
Product Listing
OriGene’s 24,000 human full-length cDNA clones have individual catalog numbers beginning with TC100001
and growing. Each clone is priced to reflect the respective cloning effort needed to produce it. All of
OriGene’s clones are priced to provide value to our customers in the scientific community.
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Introduction:
Functional genomics, the development and
application of experimental approaches to assess
gene function, begins with a full-length copy of the
gene of interest. Historically, gene cloning was done
one gene at a time through painstaking efforts from
individual laboratories. Organizations such as NCBI,
DDBJ, and EMBL have been centralizing and
archiving gene sequence information, yet they
cannot provide the physical clones needed to allow
researchers to pursue the functional dissection of
these genes, many of which still code for
hypothetical proteins having no known functions.
OriGene Technologies is focused on its mission to
clone every single human gene transcript in the
human transcriptome. We believe that a complete set
of human cDNAs provides the key to unlocking the
fundamentals of human development, physiology
and disease. Since 1997, OriGene Technologies has
been diligent in isolating full-length human genes
from primary cDNA libraries. Utilizing
high-throughput cloning and proprietary Rapid-Screen™
technologies, OriGene has built its TrueClone
TMCollection — the world’s largest searchable set of
over 24,000 non-redundant full-length human cDNA
clones suitable for protein expression in mammalian
cells or
in vitro
through coupled translation assays.
All TrueClones are matched to annotated mRNA
reference sequences found in the public domain.
Beginning in 2001, OriGene was one of the first
com-panies to provide ready-cloned genes to the research
community. Our dependable service and dedicated
support have relieved many scientists of the tedious
work of traditional gene cloning. Today, purchasing a
clone has become the standard way of obtaining a
gene; it greatly shortens the gene-cloning timetable
and allows scientists to concentrate their efforts and
resources on exciting downstream applications.
Most importantly, the availability of a large number
of expression-ready human full-length cDNAs makes
it possible to perform global screening of the entire
human transcriptome. Such a systematic approach
has been pioneered by several groups and has
shown to be extremely effective
1-8. In one recent
success story, Novartis scientists utilized OriGene’s
TrueClone Collection to identify novel gene
regulators of the WNT signaling pathway
1. OriGene is
releasing several such gene array tools to provide
this enabling technology to researchers. The
FlagArray
TM(
F
ull-
L
ength cDNA
A
nnotation of
G
ene
function array) is one such tool.
1. Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis
Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1927-32. (Use of over 10,000 OriGene TrueClones)
2. High throughput functional genomics: identification of novel genes with tumor suppressor phenotypes.
Int J Cancer. 2005 Jan 20;113(3):434-9.(Use of over 17,000 clones)
3. High-throughput Functional Genomics Identifies Genes That Ameliorate Toxicity Due to Oxidative Stress in Neuronal HT-22 Cells: GFPT2 Protects Cells Against Peroxide
Mol Cell Proteomics. 2004 Aug;3(8):834-40.(Use of 5,000 clones, including OriGene's TrueClones)
4. Identification of p53 regulators by genome-wide functional analysis
Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3456-61. (Use of 20,000 clones)
5. Genome-scale functional profiling of the mammalian AP-1 signaling pathway.
Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12153-8.(Use of 20,000 clones) 6. Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells.
Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12147-52.(Use of 20,000 clones) 7. An integrated functional genomics screening program reveals a role for BMP-9 in glucose homeostasis.
Nat Biotechnol. 2003 Mar;21(3):294-301. (Use of 8,000 clones encoding secreted proteins)
8. TIP, a T-cell factor identified using high-throughput screening increases survival in a graft-versus-host disease model.
Nat Biotechnol. 2003 Mar;21(3):302-7. (Use of 8,000 ESTs encoding putative secreted proteins)
Product Description:
What is a TrueClone?
TrueClone is the world’s largest collection of
full-length human cDNA clones, and is available only
from OriGene. The TrueClone Collection has the
following key features:
1.
Comprehensive collection:
The TrueClone
col-lection currently contains over 24,000
non-redundant human cDNAs, each corresponding to
a unique transcript. It is by far the largest source
of human cDNA clones. OriGene has the
commitment and the technical strength to clone
every single human gene.
2.
Authentic clones:
More than 99% of the clones are
directly isolated from primary cDNA libraries with
no PCR-introduced artifacts. A very small number of
clones are generated by PCR and these are all fully
sequenced to match to their references. These PCR
clones are noted as such on the OriGene website.
3.
Expression ready:
All genes are directionally
cloned into an expression vector under control of
the CMV promoter, ready to be transfected into
mammalian cells for protein expression. The
uniform vector in all clones facilitates
high-throughput screening approaches.
4.
Easy search:
OriGene provides several search
engines on our website for convenient identification
of your gene of interest. Please refer to page 7 to see
more search information.
5.
Quick delivery:
Most TrueClones will be delivered
to you as dried, plasmid DNA within 48 hrs in 2-D
bar-coded tubes.
How is a full-length cDNA defined
in OriGene’s TrueClone collection?
Full-length is usually defined as containing the full
open reading frame (ORF) of a gene, but OriGene
TrueClones contain 5’- and 3’-UTRs as well. Currently
OriGene’s clones are defined as full-length when
they satisfy the following three specifications.
1. 5’-junction sequencing: The sequence aligns
upstream of the initiation ATG codon.
2. 3’-junction sequencing: The sequence aligns
downstream of the stop codon.
3. Size verification: Each insert is removed from the
vector by restriction digestion and the size is
esti-mated via gel electrophoresis. This size is matched
against the predicted size of the full-length clone.
OriGene makes all 5’ sequence data available
through its website to facilitate customer searches. In
addition, OriGene has sequenced the ORF of more
than 2,000 TrueClones, including most of the protein
kinases, and has made this information available on
our website. The number of ORF sequenced clones
will increase steadily in the coming years.
How is a non-PCR clone different from a
PCR-based clone? Can PCR be used in
TrueClone’s downstream applications?
PCR clones may contain mutations that cause
downstream experimental deviations. Non-PCR clones
reflect the true nature of mRNA gene transcripts.
It is a well-documented fact that the PCR process
introduces mutations due to the limited fidelity of Taq
or other PCR enzymes. When a gene is obtained by
the PCR process from a cDNA pool, especially when
the transcript is rare, the high replication cycle
causes the accumulation of mutations. However, this
mutation rate can pale in comparison to the
introduction of mutations due to primer synthesis
errors that can affect 14% of all such PCR clones
9.
Even when the final transcripts are sequenced, there
is no way to differentiate whether a mutation is due
to PCR or is simply a naturally occurring SNP. In
addition, very long genes and GC-rich genes can be
impossible to PCR amplify.
One key feature of TrueClones is that they are
authentic clones, isolated directly from cDNA
libraries constructed by a high-fidelity reverse
transcriptase. When using TrueClones, there is no
need to worry about PCR-introduced artifacts.
PCR can be utilized in TrueClone applications.
Starting with an authentic cDNA clone and knowing
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its sequence, molecular biologists can safely apply
PCR to amplify a segment of the clone, either the ORF
or a functional domain, for other applications. Using
larger starting amounts of the template DNA, PCR
can be performed with a very low cycle number,
reducing the chance of mutation. More importantly,
by having the sequence information of the template
DNA, it is easy to verify the sequence of the final
product and then to distinguish any PCR-introduced
mutations from naturally occurring SNPs.
How many genes are there in the human
genome? Which commercial source offers
the most complete set of human
transcript clones?
The most recent human genome build (#35) boasts a
large scale improvement over all previous builds and
is likely to become the working genome for quite
some time, providing a more stable gene reference
set. While the total number of gene loci is currently
believed to be 21,787 (2,188 predicted), the expected
number of transcripts including splice variants is
expected to be 1.54 times that, or 33,552.
The largest commercialized collection of cDNA
clones is OriGene’s TrueClone Collection: ~24,000
transcripts covering over 15,000 loci. This is more
than twice as many as those represented in the
mammalian gene collection. Our comprehensive
collection not only provides individual gene users
the best source to find their gene, but also provides
the finest system biology tool available to assess
drug target cross-reactivity, drug action and antigen
recognition. Providing the complete human
transcriptome as non-PCR clones is and remains
OriGene’s mission.
Fig. 1 illustrates the relative relationship between the
predicted human genome and current coverage
provided by OriGene’s TrueClone collection and MGC.
OriGene’sTrueClone collection not only contains more
human full-length cDNA clones than any other
collection, but it also contains more of the difficult and
long clones. Since most cDNA collections all overlap
Fig. 1
Comparison of the predicted number of human transcripts and the
coverage of OriGene TrueClone and MGC. OriGene has more full
length cDNA clones representing a greater number of transcripts
than any other source.
*Number of Transcripts including: Genes, Predicted Genes,
Pseudo-genes and Splice Variants
26,200 (RefSeq)
24,000 OriGene
>33,000 transcripts (21,787 loci)*
Human Genome Build 35
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in their coverage of highly abundant and small cDNA
clones (ORF size less than 3kb), we looked at 200
difficult to isolate clones in this druggable genome
that have an ORF greater than 4 kb.
The result of this analysis is as follows: OriGene has
about 120 of these 200 cDNAs. One of the larger
commercial reagent suppliers has about 27 of these
and the MGC collection has 17 cDNAs representing
these 200 genes. (Fig. 2)
What vectors are employed by TrueClones,
and how do they compare to other
expression vectors?
All of the cDNAs within the TrueClone collection are
cloned into a series of pCMV6 vectors, allowing for
uniform expression screening methods. The vector
plasmid was originally obtained from Dr. David
Russell’s laboratory
10,11. The full-length cDNA
frag-ments are directionally inserted downstream from a
eukaryotic transcriptional promoter capable of
driving heterologous gene expression in a variety of
mammalian cell lines and transgenic mice.
Vector Diagram
All three OriGene pCMV6 vectors were constructed
with the same features and multiple cloning site
(MCS), with the exception that pCMV-XL6 has an SP6
transcriptional promoter instead of T7. The basic
backbone of the vector series is presented in the
diagram to the left. (Fig. 3)
Extensive work has been done to engineer the vector
to achieve a high level of transgene expression.
When compared with the popular expression
plasmid, pCDNA3.1 (Invitrogen), pCMV-based
plasmids provide comparable if not higher levels of
transgene expression. (Fig. 4)
Key Functional Features of pCMV6 vectors:
•
Vector size:
4.7kb
•
Selection marker in E. coli:
Ampicillin-resistance
•
Selection marker in mammalian cells
: None;
requires co-transfection for selection; perfect for
transient transfections
•
Promoter for in vivo expression in mammalian
cells:
CMV promoter
10. Cloning, structure, and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.
J Biol Chem. 1989 May 15; 264(14):8222-9.
11. Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation.
J Biol Chem. 1989 Sep 25; 264(27):16249-55.
Comparison of transgene expression levels in pCMV6- and
pCDNA3.1-based plasmids. CAT gene was cloned downstream of
the promoters in the pCMV6 and pCDNA3.1 vectors. In three
independent experiments, the same quantity of plasmid DNA was
transfected into Hela cells and the CAT activity was scored.
T7 Promoter Sac I Not I EcoR I# Bgl II Kpn I EcoRV Hind III Sal I#* Xba I Not I Sma I # Cloning sites *Lost upon cloning f1 ori Amp resistance gene ColE1 SV40 ori PolyA signal cmv promoter pCMV6-XL4 4707 bp
Fig. 3
Fig. 4
MCSFig. 2
Druggable Genome extracted from information provided by Hopkins,
AL, and Groom, CR, The Druggable Genome,
Nat Rev Drug Discov.
2002 9:727-30. In our evaluation, the Druggable Genome consists of
over 3800 unique genes, of which 200 are larger than 4 kb in length.
The comparison above is based upon those large genes.
Fig. 5
Sample applications for individual TrueClone.
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•
Promoter for in vitro cell free system:
T7 (for
pCMV6-XL4 and pCMV6-XL5) and SP6 for (pCMV6-XL6)
•
Cloning sites:
EcoRI and SalI; while EcoRI is
preserved, SalI is destroyed upon cloning
•
Restriction sites for removing insert:
NotI. Two NotI
sites are flanking the vector’s cloning sites
•
Cell lines suitable for transfection:
COS, HEK293,
Hela, CHO, NIH3T3, Mouse L cell, etc.
•
Transcription termination and poly-adenylation
signals:
From human growth hormone (hGH) gene
The complete sequence of pCMV6-XL4 has been
de-posited in Genbank with accession number AF067196.
All vector sequences are available at www.origene.com.
What are the utilities of the TrueClone?
TrueClones can be utilized as:
• Individual clones
• Bundled clones
• A complete collection
Individual TrueClones enable a wide spectrum of
gene function studies, as shown in Fig. 5.
Bundles of TrueClone can be selected to perform
focused screening for ligand binding, protein
interaction, or pathway dissection. The bundle can be
constructed based on gene families, such as protein
kinases, GPCRs, nuclear hormone receptors, etc, or it
can be based on functional or cellular localization
similarities, such as secreted molecules, membrane
proteins, etc. OriGene has grouped some key gene
families into CloneSets™ to facilitate such needs.
The complete TrueClone collection can be used to
perform global screening. The comprehensive nature
of the TrueClone and its uniform expression vector
platform make it an ideal tool for a system biology
approach. Exciting new discoveries
12, 13have been
recently made using the TrueClone collection.
How do I search for a gene?
OriGene’s website www.origene.com provides a
wide choice of search criteria.
12. Identification of the Wnt signaling activator leucine-rich repeat in Flightless interaction protein 2 by a genome-wide functional analysis.
Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1927-32.
13. High-throughput Functional Genomics Identifies Genes That Ameliorate Toxicity Due to Oxidative Stress in Neuronal HT-22 Cells: GFPT2 Protects Cells Against Peroxide
Mol Cell Proteomics. 2004 Aug;3 (8):834-40.
Producing siRNA by
in vitro
transcription
and dicer digestion
Subclone into
viral vector for
virus production
(eg. Adenovirus,
Lentivirus, etc)
protein
expression
in cell-free
system
(eg. TNT)
Transient
in vivo
protein
expression
Subcloning into
other expression
vectors for tagged
expression
TrueClone
Knock-in
construct for
transgenic
experiment
Transfection of
mammalian cells
Antibody validation
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•
BLAST
by Nucleotide Sequence, or BLAST by
Protein Sequence
The most sensitive search method is via
BLAST. It generates the most definitive match
to your gene of interest.
•
Accession or Catalog Number
The most specific method is by NCBI
accession number. OriGene generally uses the
RefSeq reference number beginning with NM
or XM whenever it is available.
•
Protein Domain
An effective method to extract lists of genes
that share a common protein domain.
•
Keywords
Keywords are the most flexible but can also
fail to give the desired result. False negative
results are common and it is recommended
that a BLAST search be performed after a
negative keyword search.
•
Gene Family
You can view OriGene’s pre-bundled
Clone-Sets™ by gene family from this selection.
If you have difficulty searching or finding what you
are looking for, please call our Customer Care Team
at 888.267.4436 or write to Search Help at OriGene
Will OriGene provide a custom
cloning service whenever a search
result is negative?
OriGene is dedicated to clone every human
transcript and we can incorporate your needs in this
effort. When your search result is negative, you are
encouraged to fill out a “Customer Cloning Request
Form” so that our cloning team can put your clone
into our cloning queue. Given the large number of
such requests, we prioritize those customers who
make a commitment to purchase once the clone is
available. You can make such a commitment by
submitting a purchase order. To obtain a quotation,
please contact Customer Care at 1-888-267-4436.
Why are TrueClones priced differently?
Not all clones are created equally. Gene transcripts
differ greatly in many aspects, such as message
abundance, transcript length, GC content, and tissue
distribution. The respective cloning efforts needed to
produce them differ accordingly. OriGene’s
differential pricing reflects the relative difficulty in
obtaining these clones, while still providing value to
all clone users.
Some of the clones represent short and very abundant
transcripts, such as the beta-actin transcript. Such
clones can be obtained with relative ease by
high-throughput sequencing of a small number of clones in
the libraries. Most of the clones in MGC represent such
transcripts. The majority of the TrueClone collection
categorized within this group are priced at $195.
Large and rare clones are difficult to clone and
require individualized effort. Equipped with the
proprietary Rapid-Screen
TMtechnology, OriGene has
been able to obtain more elusive clones than any
other cloning team, and thus can provide a much
more comprehensive collection. We are continuing
to apply new technologies to clone more genes and
to expand our collection. We believe our offering
provides an excellent value to our customers.
What is provided when I order a gene?
1. OriGene provides purified plasmid DNA (200ng)
dried onto the bottom of a siliconized
poly-propylene tube. The tube has a 2-D bar code on the
bottom which ensures inventory integrity and
accurate shipping, and allows for potential
downstream integration into the end-users LIMS.
2. A copy of our TrueClone Application Guide with
information regarding DNA recovery and
down-stream applications.
3. A certificate of analysis stating your clone’s
specifications.
4. A vial of vector primer (vp1.5) for 5’ sequence
confirmation.
Will OriGene guarantee that a TrueClone
is identical to a published reference?
No. There are three factors that contribute to
differences:
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1.
Single Nucleotide Polymorphisms (SNP’s).
No two
collections will contain exactly the same gene
sequences, and this is true when comparing the
NCBI database references with OriGene’s
True-Clones. Given that polymorphisms exist in 0.1% of
the euchromatic genome, we can expect there to
be a 1 bp difference in each 1000 bp of a reference
on average. Obtained directly from human primary
cDNA libraries, TrueClones are the closest copies to
actual human mRNA transcripts that technology
currently allows, and therefore any such difference
in a TrueClone is likely to be a single nucleotide
polymorphism (SNP).
2.
Reference sequences are not always correct.
Some
references contain PCR-cloning errors and/or
sequencing errors. In fact, less than one-half of the
curated NCBI RefSeq database is currently placed
into the “reviewed, validated” category.
3.
Alternative splicing.
Most of OriGene’s clones are
not yet fully sequenced. We qualify our clones by
5’- and 3‘-end sequence matching and insert size
verification. It is possible that some TrueClones
contain a different splice pattern than the
reference.
OriGene guarantees that TrueClones are authentic
cDNA transcripts that encode full-length proteins. If
any of our clones are shown to contain a mutation/
insertion/deletion that disrupts the protein’s open
reading frame, every effort will be made to supply an
appropriate replacement clone from our very large
archive of full-length candidates.
For protein purification, do OriGene
TrueClones provide a fused tag to
the expressed protein?
Currently TrueClones are “raw” clones from libraries
supplied without any fused tag. Customers are
encouraged to perform the vector construction in
their own labs. To satisfy such needs for tagged
protein expression, OriGene has teamed up with
Promega to develop a new line of TrueClone
derivatives—the FlexClone series. Flex clones will
enable convenient shuffling of an ORF into a variety
of vectors, facilitating multiple applications,
including tagged protein expression.
Which cloning sites were used to
create TrueClones? Which restriction
sites can be used to remove the insert
from a TrueClone?
The cDNA prepared for each library construction was
engineered to have an EcoR1 site on the 5’ end and
an Xho1 site on the 3’ end. The fragments were
ligated into the pCMV vectors pre-cut with EcoR1 and
Sal1. The compatible ends of Xho1 and Sal1 created
a new site that cannot be cut with either.
To liberate the insert, use NotI. NotI sites were
engineered on both sides of the cloning sites.
Please refer to page 6 for more vector information.
What additional information is provided
on OriGene’s website when I search for
a clone?
OriGene is striving to be your one-stop gene cloning
resource. When you search for a gene on our site, we
provide:
• Direct links to public databases for more detailed
information about each gene
• Sequence specifications for the OriGene clone
• A 24-tissue expression profile: Relative
expression levels of this gene in 24-normal
tissues (data obtained by RT-PCR using OriGene’s
Rapid-Scan expression panels)
• All available products related to this gene,
including protein, antibody and pre-made shRNA
constructs
• The gene family, pathway and function summary
for your gene
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What is a CloneSet™? How many
CloneSets™ does OriGene have? What
are the utilities of a CloneSet™?
CloneSets™ are pre-bundled clones based on gene
families. Currently there are several such
Clone-Sets™ within OriGene’s TrueClone collection:
• Protein kinase
• GPCR (G-protein coupled receptors)
• NHR (Nuclear Hormone receptors)
• P450
• Ion Channel
OriGene has created, by far, the most
com-prehensive gene family collections available in the
marketplace, and is proud to offer these important
CloneSets™ to the research community. Such
categorization assists researchers with focused
scientific interest. CloneSets™ are available as a
single product item for focused functional screening.
OriGene CloneSets™ in development include:
• Phosphatases
• Protease
• Lipid kinases
• Phosphodiesterases
• Membrane proteins
• Secreted molecules
To purchase a complete CloneSet, please contact us
at 1.888.267.4436 for terms and quotation.
CloneSet™: Gene-family
based collection
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Kinase CloneSet
Cat. No. Accession No. Description
TC107659 AB000409 Homo sapiens mRNA for MNK1, complete cds TC107660 AB023216 Homo sapiens mRNA for KIAA0999 protein, partial cds TC107655 AB037781 Homo sapiens mRNA for KIAA1360 protein, partial cds TC107442 AB058693 Homo sapiens mRNA for KIAA1790 protein, partial cds
TC107661 AF308302 Homo sapiens serologically defined breast cancer antigen NY-BR-96 mRNA, complete cds TC105333 AK074305 Homo sapiens cDNA FLJ23725 fis, clone HEP14024
TC104797 AK092352 Homo sapiens cDNA FLJ35033 fis, clone OCBBF2016590, weakly similar to CELL SURFACE ANTIGEN 114/A10 PRECURSOR
TC124162 AY792511 Homo sapiens leucine-rich repeat kinase 2 (LRRK2) mRNA, complete cds
TC124163 AY792511 Homo sapiens leucine-rich repeat kinase 2 (LRRK2) mRNA, OriGene unique variant 1 TC124164 AY792511 Homo sapiens leucine-rich repeat kinase 2 (LRRK2) mRNA, OriGene unique variant 2 TC123778 BC002695 Homo sapiens, Similar to KIAA1048 protein, clone MGC:3535 IMAGE:3607681, complete cds TC107754 BC002695 Homo sapiens, Similar to KIAA1048 protein, clone MGC:3535 IMAGE:3607681, complete cds TC107753 BC038807 Homo sapiens, Similar to amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 7,
clone MGC:46456 IMAGE:5200755, complete cds
TC107656 BC039243 Homo sapiens, Similar to fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss syndrome), clone MGC:32962 IMAGE:5277896, com
TC107755 BC041668 Homo sapiens, Similar to receptor-interacting serine-threonine kinase 3, clone MGC:47689 IMAGE: 5767146, complete cds
TC107662 BC052239 Homo sapiens cDNA clone IMAGE:5092935, partial cds TC116664 NM_000020 Homo sapiens activin A receptor type II-like 1 (ACVRL1) TC115442 NM_000061 Homo sapiens Bruton agammaglobulinemia tyrosine kinase (BTK) TC112998 NM_000075 Homo sapiens cyclin-dependent kinase 4 (CDK4)
TC113109 NM_000075 Homo sapiens cyclin-dependent kinase 4 (CDK4), OriGene unique variant 1
TC111932 NM_000141 Homo sapiens fibroblast growth factor receptor 2 (bacteria-expressed kinase, keratinocyte growth factor receptor, craniofacial dysostosis 1, Crouzon syndrome, Pfeiffer syndrome, Jackson-Weiss syndrome) (FGFR2), transcript variant 1
TC109231 NM_000142 Homo sapiens fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) (FGFR3), transcript variant 1
TC109267 NM_000162 Homo sapiens glucokinase (hexokinase 4, maturity onset diabetes of the young 2) (GCK), transcript variant 1
TC120059 NM_000215 Homo sapiens Janus kinase 3 (a protein tyrosine kinase, leukocyte) (JAK3) TC120061 NM_000222 Homo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT) TC124046 NM_000245 Homo sapiens met proto-oncogene (hepatocyte growth factor receptor) (MET) TC119991 NM_000294 Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2)
TC119992 NM_000294 Homo sapiens phosphorylase kinase, gamma 2 (testis) (PHKG2), OriGene unique variant 1 TC119871 NM_000455 Homo sapiens serine/threonine kinase 11 (Peutz-Jeghers syndrome) (STK11)
TC119843 NM_000459 Homo sapiens TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous and mucosal) (TEK)
TC109227 NM_000604 Homo sapiens fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome) (FGFR1), OriGene unique variant 1
TC124152 NM_000875 Homo sapiens insulin-like growth factor 1 receptor (IGF1R)
TC119602 NM_000906 Homo sapiens natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) (NPR1)
TC107274 NM_001079 Homo sapiens zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1 TC119434 NM_001105 Homo sapiens activin A receptor, type I (ACVR1)
TC119435 NM_001106 Homo sapiens activin A receptor, type IIB (ACVR2B)
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Cat. No. Accession No. Description
TC108987 NM_001204 Homo sapiens bone morphogenetic protein receptor, type II (serine/threonine kinase) (BMPR2), transcript variant 1
TC119395 NM_001211 Homo sapiens BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B) TC108757 NM_001221 Homo sapiens calcium/calmodulin-dependent protein kinase (CaM kinase) II delta (CAMK2D),
transcript variant 3
TC119340 NM_001258 Homo sapiens cyclin-dependent kinase 3 (CDK3), OriGene unique variant 1 TC119343 NM_001260 Homo sapiens cyclin-dependent kinase 8 (CDK8)
TC119344 NM_001261 Homo sapiens cyclin-dependent kinase 9 (CDC2-related kinase) (CDK9)
TC119354 NM_001274 Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1), OriGene unique variant 1 TC119355 NM_001274 Homo sapiens CHK1 checkpoint homolog (S. pombe) (CHEK1)
TC119358 NM_001278 Homo sapiens conserved helix-loop-helix ubiquitous kinase (CHUK)
TC124169 NM_001315 Homo sapiens mitogen-activated protein kinase 14 (MAPK14), transcript variant 1 TC119302 NM_001319 Homo sapiens casein kinase 1, gamma 2 (CSNK1G2)
TC119321 NM_001348 Homo sapiens death-associated protein kinase 3 (DAPK3) TC119139 NM_001569 Homo sapiens interleukin-1 receptor-associated kinase 1 (IRAK1) TC110934 NM_001570 Homo sapiens interleukin-1 receptor-associated kinase 2 (IRAK2) TC119156 NM_001616 Homo sapiens activin A receptor, type II (ACVR2)
TC119158 NM_001619 Homo sapiens adrenergic, beta, receptor kinase 1 (ADRBK1)
TC119161 NM_001626 Homo sapiens v-akt murine thymoma viral oncogene homolog 2 (AKT2) TC119127 NM_001654 Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog (ARAF) TC119055 NM_001715 Homo sapiens B lymphoid tyrosine kinase (BLK), OriGene unique variant 1 TC119056 NM_001715 Homo sapiens B lymphoid tyrosine kinase (BLK)
TC119060 NM_001721 Homo sapiens BMX non-receptor tyrosine kinase (BMX)
TC110839 NM_001726 Homo sapiens bromodomain, testis-specific (BRDT), transcript variant 2 TC119077 NM_001744 Homo sapiens calcium/calmodulin-dependent protein kinase IV (CAMK4) TC111605 NM_001786 Homo sapiens cell division cycle 2, G1 to S and G2 to M (CDC2), transcript variant 1 TC119016 NM_001787 Homo sapiens cell division cycle 2-like 1 (PITSLRE proteins) (CDC2L1), transcript variant 1 TC109059 NM_001798 Homo sapiens cyclin-dependent kinase 2 (CDK2), OriGene unique variant 1
TC109060 NM_001798 Homo sapiens cyclin-dependent kinase 2 (CDK2), transcript variant 1
TC119024 NM_001799 Homo sapiens cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) (CDK7) TC118967 NM_001892 Homo sapiens casein kinase 1, alpha 1 (CSNK1A1)
TC118968 NM_001893 Homo sapiens casein kinase 1, delta (CSNK1D), OriGene unique variant 1 TC109127 NM_001895 Homo sapiens casein kinase 2, alpha 1 polypeptide (CSNK2A1), transcript variant 2 TC118969 NM_001896 Homo sapiens casein kinase 2, alpha prime polypeptide (CSNK2A2)
TC123974 NM_001954 Homo sapiens discoidin domain receptor family, member 1 (DDR1), transcript variant 2
TC118918 NM_001982 Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) (ERBB3), transcript variant 1
TC118883 NM_002005 Homo sapiens feline sarcoma oncogene (FES)
TC111632 NM_002011 Homo sapiens fibroblast growth factor receptor 4 (FGFR4), transcript variant 1 TC118894 NM_002020 Homo sapiens fms-related tyrosine kinase 4 (FLT4), transcript variant 2 TC118895 NM_002020 Homo sapiens fms-related tyrosine kinase 4 (FLT4), OriGene unique variant 1 TC118862 NM_002037 Homo sapiens FYN oncogene related to SRC, FGR, YES (FYN), transcript variant 1 TC108487 NM_002082 Homo sapiens G protein-coupled receptor kinase 6 (GRK6), transcript variant 2 TC118824 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B), OriGene unique variant 1 TC118825 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B), OriGene unique variant 2 TC118826 NM_002093 Homo sapiens glycogen synthase kinase 3 beta (GSK3B)
TC118838 NM_002110 Homo sapiens hemopoietic cell kinase (HCK)
TC118761 NM_002227 Homo sapiens Janus kinase 1 (a protein tyrosine kinase) (JAK1)
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Cat. No. Accession No. Description
TC118711 NM_002314 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1 TC123883 NM_002314 Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1
TC108490 NM_002350 Homo sapiens v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN), OriGene unique variant 1
TC108492 NM_002350 Homo sapiens v-yes-1 Yamaguchi sarcoma viral related oncogene homolog (LYN)
TC118702 NM_002376 Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3), OriGene unique variant 1 TC118703 NM_002376 Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3)
TC118669 NM_002401 Homo sapiens mitogen-activated protein kinase kinase kinase 3 (MAP3K3), transcript variant 2 TC110949 NM_002419 Homo sapiens mitogen-activated protein kinase kinase kinase 11 (MAP3K11)
TC118630 NM_002446 Homo sapiens mitogen-activated protein kinase kinase kinase 10 (MAP3K10) TC108482 NM_002497 Homo sapiens NIMA (never in mitosis gene a)-related kinase 2 (NEK2)
TC109479 NM_002498 Homo sapiens NIMA (never in mitosis gene a)-related kinase 3 (NEK3), OriGene unique variant 1 TC109481 NM_002498 Homo sapiens NIMA (never in mitosis gene a)-related kinase 3 (NEK3), transcript variant 1 TC118578 NM_002530 Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3)
TC118579 NM_002530 Homo sapiens neurotrophic tyrosine kinase, receptor, type 3 (NTRK3), OriGene unique variant 1 TC107950 NM_002576 Homo sapiens p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) (PAK1)
TC118562 NM_002577 Homo sapiens p21 (CDKN1A)-activated kinase 2 (PAK2) TC118563 NM_002578 Homo sapiens p21 (CDKN1A)-activated kinase 3 (PAK3) TC118532 NM_002595 Homo sapiens PCTAIRE protein kinase 2 (PCTK2)
TC118538 NM_002610 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 1 (PDK1), nuclear gene encoding mitochondrial protein, OriGene unique variant 1
TC118539 NM_002610 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 1 (PDK1), nuclear gene encoding mitochondrial protein
TC118540 NM_002611 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 2 (PDK2) TC118542 NM_002612 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 4 (PDK4)
TC118543 NM_002613 Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1), OriGene unique variant 1 TC118544 NM_002613 Homo sapiens 3-phosphoinositide dependent protein kinase-1 (PDPK1)
TC110975 NM_002648 Homo sapiens pim-1 oncogene (PIM1)
TC118448 NM_002730 Homo sapiens protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1 TC108498 NM_002731 Homo sapiens protein kinase, cAMP-dependent, catalytic, beta (PRKACB), OriGene unique variant 1 TC108499 NM_002731 Homo sapiens protein kinase, cAMP-dependent, catalytic, beta (PRKACB), transcript variant 2 TC118449 NM_002732 Homo sapiens protein kinase, cAMP-dependent, catalytic, gamma (PRKACG)
TC124082 NM_002737 Homo sapiens protein kinase C, alpha (PRKCA)
TC118454 NM_002738 Homo sapiens protein kinase C, beta 1 (PRKCB1), transcript variant 2 TC112717 NM_002739 Homo sapiens protein kinase C, gamma (PRKCG)
TC118455 NM_002740 Homo sapiens protein kinase C, iota (PRKCI)
TC118456 NM_002741 Homo sapiens protein kinase N1 (PKN1), transcript variant 2 TC118457 NM_002742 Homo sapiens protein kinase D1 (PRKD1)
TC118459 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ), OriGene unique variant 1 TC118460 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ), OriGene unique variant 2 TC118461 NM_002744 Homo sapiens protein kinase C, zeta (PRKCZ)
TC118463 NM_002747 Homo sapiens mitogen-activated protein kinase 4 (MAPK4) TC118465 NM_002748 Homo sapiens mitogen-activated protein kinase 6 (MAPK6)
TC109619 NM_002750 Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant 2 TC118466 NM_002751 Homo sapiens mitogen-activated protein kinase 11 (MAPK11), transcript variant 1 TC111675 NM_002752 Homo sapiens mitogen-activated protein kinase 9 (MAPK9), transcript variant 1 TC109625 NM_002753 Homo sapiens mitogen-activated protein kinase 10 (MAPK10), transcript variant 1 TC118423 NM_002754 Homo sapiens mitogen-activated protein kinase 13 (MAPK13)
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Cat. No. Accession No. Description
TC109629 NM_002757 Homo sapiens mitogen-activated protein kinase kinase 5 (MAP2K5), OriGene unique variant 1 TC124173 NM_002757 Homo sapiens mitogen-activated protein kinase kinase 5 (MAP2K5), transcript variant B TC118432 NM_002758 Homo sapiens mitogen-activated protein kinase kinase 6 (MAP2K6), transcript variant 1 TC118433 NM_002759 Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2) TC118434 NM_002760 Homo sapiens protein kinase, Y-linked (PRKY)
TC118391 NM_002821 Homo sapiens PTK7 protein tyrosine kinase 7 (PTK7), transcript variant PTK7-1 TC109662 NM_002822 Homo sapiens PTK9 protein tyrosine kinase 9 (PTK9), transcript variant 1 TC109663 NM_002822 Homo sapiens PTK9 protein tyrosine kinase 9 (PTK9), OriGene unique variant 1
TC118322 NM_002880 Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1), OriGene unique variant 1 TC118323 NM_002880 Homo sapiens v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1)
TC118278 NM_002929 Homo sapiens G protein-coupled receptor kinase 1 (GRK1)
TC118295 NM_002953 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 1 (RPS6KA1), transcript variant 1 TC112721 NM_002958 Homo sapiens RYK receptor-like tyrosine kinase (RYK), transcript variant 2
TC118305 NM_002969 Homo sapiens mitogen-activated protein kinase 12 (MAPK12)
TC118306 NM_002969 Homo sapiens mitogen-activated protein kinase 12 (MAPK12), OriGene unique variant 1 TC118239 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), OriGene unique variant 1 TC118240 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4)
TC118241 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), OriGene unique variant 2 TC118243 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), OriGene unique variant 3 TC118244 NM_003010 Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), OriGene unique variant 4 TC118183 NM_003137 Homo sapiens SFRS protein kinase 1 (SRPK1)
TC118137 NM_003157 Homo sapiens NIMA (never in mitosis gene a)-related kinase 4 (NEK4) TC108474 NM_003159 Homo sapiens cyclin-dependent kinase-like 5 (CDKL5), OriGene unique variant 1 TC118139 NM_003160 Homo sapiens aurora kinase C (AURKC)
TC118140 NM_003161 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1)
TC118141 NM_003161 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 1 (RPS6KB1), OriGene unique variant 1 TC118115 NM_003177 Homo sapiens spleen tyrosine kinase (SYK)
TC118119 NM_003188 Homo sapiens mitogen-activated protein kinase kinase kinase 7 (MAP3K7), transcript variant A TC118147 NM_003215 Homo sapiens tec protein tyrosine kinase (TEC)
TC118072 NM_003242 Homo sapiens transforming growth factor, beta receptor II (70/80kDa) (TGFBR2) TC118032 NM_003318 Homo sapiens TTK protein kinase (TTK)
TC118033 NM_003318 Homo sapiens TTK protein kinase (TTK), OriGene unique variant 1 TC118038 NM_003328 Homo sapiens TXK tyrosine kinase (TXK)
TC111048 NM_003331 Homo sapiens tyrosine kinase 2 (TYK2) TC117994 NM_003384 Homo sapiens vaccinia related kinase 1 (VRK1) TC117999 NM_003390 Homo sapiens WEE1 homolog (S. pombe) (WEE1)
TC117908 NM_003503 Homo sapiens CDC7 cell division cycle 7 (S. cerevisiae) (CDC7) TC117907 NM_003576 Homo sapiens serine/threonine kinase 24 (STE20 homolog, yeast) (STK24)
TC117867 NM_003582 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (DYRK3), transcript variant 1
TC109928 NM_003583 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript variant 1
TC117877 NM_003600 Homo sapiens serine/threonine kinase 6 (STK6), transcript variant 2
TC117892 NM_003618 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), OriGene unique variant 1
TC117893 NM_003618 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3) TC117843 NM_003656 Homo sapiens calcium/calmodulin-dependent protein kinase I (CAMK1)
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Cat. No. Accession No. Description
TC109943 NM_003668 Homo sapiens mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), transcript variant 1
TC109944 NM_003668 Homo sapiens mitogen-activated protein kinase-activated protein kinase 5 (MAPKAPK5), OriGene unique variant 1
TC109951 NM_003674 Homo sapiens cyclin-dependent kinase (CDC2-like) 10 (CDK10), OriGene unique variant 1 TC109952 NM_003674 Homo sapiens cyclin-dependent kinase (CDC2-like) 10 (CDK10), transcript variant 1 TC117862 NM_003684 Homo sapiens MAP kinase interacting serine/threonine kinase 1 (MKNK1)
TC117810 NM_003688 Homo sapiens calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK) TC108468 NM_003691 Homo sapiens serine/threonine kinase 16 (STK16), transcript variant 1
TC109966 NM_003718 Homo sapiens cell division cycle 2-like 5 (cholinesterase-related cell division controller) (CDC2L5), transcript variant 1
TC108466 NM_003804 Homo sapiens receptor (TNFRSF)-interacting serine-threonine kinase 1 (RIPK1) TC117734 NM_003821 Homo sapiens receptor-interacting serine-threonine kinase 2 (RIPK2)
TC117735 NM_003821 Homo sapiens receptor-interacting serine-threonine kinase 2 (RIPK2), OriGene unique variant 1 TC109987 NM_003831 Homo sapiens RIO kinase 3 (yeast) (RIOK3), transcript variant 1
TC106951 NM_003845 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), OriGene unique variant 1
TC106952 NM_003845 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4) TC117716 NM_003852 Homo sapiens transcriptional intermediary factor 1 (TIF1), transcript variant 2
TC107962 NM_003913 Homo sapiens PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), transcript variant 1 TC117648 NM_003942 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 4 (RPS6KA4), transcript variant 1 TC111095 NM_003948 Homo sapiens cyclin-dependent kinase-like 2 (CDC2-related kinase) (CDKL2)
TC117656 NM_003952 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2), OriGene unique variant 1 TC124062 NM_003952 Homo sapiens ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2), transcript variant 1 TC117658 NM_003954 Homo sapiens mitogen-activated protein kinase kinase kinase 14 (MAP3K14)
TC101068 NM_003957 Homo sapiens BR serine/threonine kinase 2 (BRSK2) TC117643 NM_003985 Homo sapiens tyrosine kinase, non-receptor, 1 (TNK1) TC109075 NM_003992 Homo sapiens CDC-like kinase 3 (CLK3), transcript variant phclk3 TC109076 NM_003992 Homo sapiens CDC-like kinase 3 (CLK3), OriGene unique variant 1hclk3 TC117645 NM_003993 Homo sapiens CDC-like kinase 2 (CLK2), transcript variant 1
TC117646 NM_003995 Homo sapiens natriuretic peptide receptor B/guanylate cyclase B (atrionatriuretic peptide receptor B) (NPR2) TC117573 NM_004071 Homo sapiens CDC-like kinase 1 (CLK1), OriGene unique variant 1
TC117574 NM_004071 Homo sapiens CDC-like kinase 1 (CLK1)
TC117577 NM_004073 Homo sapiens polo-like kinase 3 (Drosophila) (PLK3)
TC109209 NM_004103 Homo sapiens PTK2B protein tyrosine kinase 2 beta (PTK2B), transcript variant 2 TC124086 NM_004119 Homo sapiens fms-related tyrosine kinase 3 (FLT3)
TC109998 NM_004197 Homo sapiens serine/threonine kinase 19 (STK19), transcript variant 1 TC109999 NM_004197 Homo sapiens serine/threonine kinase 19 (STK19), OriGene unique variant 1
TC117519 NM_004203 Homo sapiens membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase (PKMYT1), transcript variant 1
TC117533 NM_004226 Homo sapiens serine/threonine kinase 17b (apoptosis-inducing) (STK17B) TC108895 NM_004302 Homo sapiens activin A receptor, type IB (ACVR1B), transcript variant 1 TC117431 NM_004327 Homo sapiens breakpoint cluster region (BCR), transcript variant 1 TC117433 NM_004329 Homo sapiens bone morphogenetic protein receptor, type IA (BMPR1A) TC117437 NM_004333 Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF)
TC110841 NM_004336 Homo sapiens BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) (BUB1) TC117389 NM_004383 Homo sapiens c-src tyrosine kinase (CSK)
TC108512 NM_004384 Homo sapiens casein kinase 1, gamma 3 (CSNK1G3) TC117404 NM_004409 Homo sapiens dystrophia myotonica-protein kinase (DMPK)
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Cat. No. Accession No. Description
TC117346 NM_004431 Homo sapiens EPH receptor A2 (EPHA2) TC121913 NM_004431 Homo sapiens EPH receptor A2 (EPHA2) TC117354 NM_004438 Homo sapiens EPH receptor A4 (EPHA4)
TC111626 NM_004439 Homo sapiens EPH receptor A5 (EPHA5), transcript variant 1 TC108415 NM_004440 Homo sapiens EPH receptor A7 (EPHA7)
TC117355 NM_004441 Homo sapiens EPH receptor B1 (EPHB1) TC117356 NM_004443 Homo sapiens EPH receptor B3 (EPHB3) TC117357 NM_004444 Homo sapiens EPH receptor B4 (EPHB4) TC117358 NM_004445 Homo sapiens EPH receptor B6 (EPHB6)
TC125233 NM_004448 Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1
TC117313 NM_004517 Homo sapiens integrin-linked kinase (ILK)
TC117279 NM_004560 Homo sapiens receptor tyrosine kinase-like orphan receptor 2 (ROR2)
TC117289 NM_004579 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 2 (MAP4K2)
TC111005 NM_004586 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3), OriGene unique variant 1 TC111006 NM_004586 Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 3 (RPS6KA3)
TC108520 NM_004612 Homo sapiens transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kDa) (TGFBR1)
TC117238 NM_004635 Homo sapiens mitogen-activated protein kinase-activated protein kinase 3 (MAPKAPK3) TC111727 NM_004672 Homo sapiens mitogen-activated protein kinase kinase kinase 6 (MAP3K6)
TC117200 NM_004690 Homo sapiens LATS, large tumor suppressor, homolog 1 (Drosophila) (LATS1)”
TC117223 NM_004714 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1B (DYRK1B), transcript variant a
TC117227 NM_004721 Homo sapiens mitogen-activated protein kinase kinase kinase 13 (MAP3K13) TC108525 NM_004734 Homo sapiens doublecortin and CaM kinase-like 1 (DCAMKL1)
TC117159 NM_004759 Homo sapiens mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), transcript variant 1
TC117160 NM_004760 Homo sapiens serine/threonine kinase 17a (apoptosis-inducing) (STK17A) TC117141 NM_004783 Homo sapiens TAO kinase 2 (TAOK2)
TC117132 NM_004836 Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3) TC117098 NM_004850 Homo sapiens Rho-associated, coiled-coil containing protein kinase 2 (ROCK2) TC117039 NM_004935 Homo sapiens cyclin-dependent kinase 5 (CDK5)
TC110866 NM_004938 Homo sapiens death-associated protein kinase 1 (DAPK1)
TC110899 NM_004958 Homo sapiens FK506 binding protein 12-rapamycin associated protein 1 (FRAP1) TC117052 NM_004963 Homo sapiens guanylate cyclase 2C (heat stable enterotoxin receptor) (GUCY2C) TC117059 NM_004972 Homo sapiens Janus kinase 2 (a protein tyrosine kinase) (JAK2)
TC117005 NM_005012 Homo sapiens receptor tyrosine kinase-like orphan receptor 1 (ROR1) TC110978 NM_005030 Homo sapiens polo-like kinase 1 (Drosophila) (PLK1)
TC116959 NM_005044 Homo sapiens protein kinase, X-linked (PRKX) TC116928 NM_005104 Homo sapiens bromodomain containing 2 (BRD2) TC111128 NM_005109 Homo sapiens oxidative-stress responsive 1 (OXSR1)
TC116914 NM_005157 Homo sapiens v-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1), transcript variant a TC116916 NM_005160 Homo sapiens adrenergic, beta, receptor kinase 2 (ADRBK2)
TC116917 NM_005160 Homo sapiens adrenergic, beta, receptor kinase 2 (ADRBK2), OriGene unique variant 1 TC116883 NM_005163 Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1)
TC112674 NM_005204 Homo sapiens mitogen-activated protein kinase kinase kinase 8 (MAP3K8)
TC116882 NM_005211 Homo sapiens colony stimulating factor 1 receptor, formerly McDonough feline sarcoma viral (v-fms) oncogene homolog (CSF1R)
TC110878 NM_005228 Homo sapiens epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) (EGFR), transcript variant 1
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Cat. No. Accession No. Description
TC116861 NM_005232 Homo sapiens EPH receptor A1 (EPHA1)
TC109197 NM_005233 Homo sapiens EPH receptor A3 (EPHA3), transcript variant 1
TC116863 NM_005235 Homo sapiens v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) (ERBB4), OriGene unique variant 1
TC116869 NM_005246 Homo sapiens fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) (FER) TC116870 NM_005248 Homo sapiens Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR) TC116876 NM_005255 Homo sapiens cyclin G associated kinase (GAK)
TC116815 NM_005307 Homo sapiens G protein-coupled receptor kinase 4 (GRK4), OriGene unique variant 1 TC110910 NM_005308 Homo sapiens G protein-coupled receptor kinase 5 (GRK5)
TC116769 NM_005356 Homo sapiens lymphocyte-specific protein tyrosine kinase (LCK), OriGene unique variant 1 TC116770 NM_005356 Homo sapiens lymphocyte-specific protein tyrosine kinase (LCK)
TC110971 NM_005391 Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 3 (PDK3) TC116792 NM_005400 Homo sapiens protein kinase C, epsilon (PRKCE)
TC116752 NM_005417 Homo sapiens v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC), transcript variant 1
TC116728 NM_005424 Homo sapiens tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1) TC116734 NM_005433 Homo sapiens v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 (YES1)
TC110105 NM_005465 Homo sapiens v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma) (AKT3), transcript variant 1
TC116679 NM_005546 Homo sapiens IL2-inducible T-cell kinase (ITK)
TC116659 NM_005569 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a TC116680 NM_005592 Homo sapiens muscle, skeletal, receptor tyrosine kinase (MUSK) TC116628 NM_005607 Homo sapiens PTK2 protein tyrosine kinase 2 (PTK2), transcript variant 2 TC116597 NM_005627 Homo sapiens serum/glucocorticoid regulated kinase (SGK)
TC116599 NM_005627 Homo sapiens serum/glucocorticoid regulated kinase (SGK), OriGene unique variant 1 TC124179 NM_005734 Homo sapiens homeodomain interacting protein kinase 3 (HIPK3)
TC116515 NM_005762 Homo sapiens tripartite motif-containing 28 (TRIM28)
TC116528 NM_005781 Homo sapiens tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 1 TC116506 NM_005813 Homo sapiens protein kinase D3 (PRKD3)
TC116464 NM_005881 Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK) TC116439 NM_005906 Homo sapiens male germ cell-associated kinase (MAK)
TC116450 NM_005923 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5) TC116404 NM_005975 Homo sapiens PTK6 protein tyrosine kinase 6 (PTK6)
TC124087 NM_005975 Homo sapiens PTK6 protein tyrosine kinase 6 (PTK6) TC116411 NM_005990 Homo sapiens serine/threonine kinase 10 (STK10)
TC116371 NM_006035 Homo sapiens CDC42 binding protein kinase beta (DMPK-like) (CDC42BPB) TC116294 NM_006182 Homo sapiens discoidin domain receptor family, member 2 (DDR2) TC111664 NM_006201 Homo sapiens PCTAIRE protein kinase 1 (PCTK1), transcript variant 1
TC116256 NM_006206 Homo sapiens platelet-derived growth factor receptor, alpha polypeptide (PDGFRA) TC116261 NM_006213 Homo sapiens phosphorylase kinase, gamma 1 (muscle) (PHKG1)
TC116246 NM_006251 Homo sapiens protein kinase, AMP-activated, alpha 1 catalytic subunit (PRKAA1), transcript variant 1 TC116247 NM_006252 Homo sapiens protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2)
TC116249 NM_006254 Homo sapiens protein kinase C, delta (PRKCD), transcript variant 1 TC116250 NM_006255 Homo sapiens protein kinase C, eta (PRKCH)
TC108568 NM_006256 Homo sapiens protein kinase N2 (PKN2) TC116251 NM_006257 Homo sapiens protein kinase C, theta (PRKCQ)
TC116252 NM_006258 Homo sapiens protein kinase, cGMP-dependent, type I (PRKG1) TC116254 NM_006259 Homo sapiens protein kinase, cGMP-dependent, type II (PRKG2) TC116188 NM_006281 Homo sapiens serine/threonine kinase 3 (STE20 homolog, yeast) (STK3)
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Cat. No. Accession No. Description
TC116189 NM_006282 Homo sapiens serine/threonine kinase 4 (STK4) TC116192 NM_006285 Homo sapiens testis-specific kinase 1 (TESK1) TC108283 NM_006293 Homo sapiens TYRO3 protein tyrosine kinase (TYRO3) TC116202 NM_006296 Homo sapiens vaccinia related kinase 2 (VRK2)
TC116203 NM_006296 Homo sapiens vaccinia related kinase 2 (VRK2), OriGene unique variant 1
TC116209 NM_006301 Homo sapiens mitogen-activated protein kinase kinase kinase 12 (MAP3K12), OriGene unique variant 1 TC116132 NM_006343 Homo sapiens c-mer proto-oncogene tyrosine kinase (MERTK)
TC111155 NM_006374 Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast) (STK25)
TC111156 NM_006374 Homo sapiens serine/threonine kinase 25 (STE20 homolog, yeast) (STK25), OriGene unique variant 1 TC109929 NM_006482 Homo sapiens dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (DYRK2), transcript
variant 2
TC124175 NM_006549 Homo sapiens calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), transcript variant 1
TC116033 NM_006549 Homo sapiens calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), OriGene unique variant 1
TC116034 NM_006549 Homo sapiens calcium/calmodulin-dependent protein kinase kinase 2, beta (CAMKK2), OriGene unique variant 2
TC110237 NM_006575 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 5 (MAP4K5), transcript variant 1 TC115980 NM_006609 Homo sapiens mitogen-activated protein kinase kinase kinase 2 (MAP3K2)
TC115988 NM_006622 Homo sapiens polo-like kinase 2 (Drosophila) (PLK2) TC115926 NM_006712 Homo sapiens FAST kinase (FASTK), transcript variant 1 TC101252 NM_006742 Homo sapiens protein serine kinase H1 (PSKH1) TC115810 NM_006852 Homo sapiens tousled-like kinase 2 (TLK2)
TC115811 NM_006852 Homo sapiens tousled-like kinase 2 (TLK2), OriGene unique variant 1 TC115812 NM_006852 Homo sapiens tousled-like kinase 2 (TLK2), OriGene unique variant 2 TC115825 NM_006875 Homo sapiens pim-2 oncogene (PIM2)
TC110233 NM_007079 Homo sapiens protein tyrosine phosphatase type IVA, member 3 (PTP4A3), transcript variant 2 TC115660 NM_007170 Homo sapiens testis-specific kinase 2 (TESK2)
TC115668 NM_007181 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1)
TC115669 NM_007181 Homo sapiens mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1), OriGene unique variant 1
TC110240 NM_007194 Homo sapiens CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1 TC115680 NM_007199 Homo sapiens interleukin-1 receptor-associated kinase 3 (IRAK3)
TC115629 NM_007271 Homo sapiens serine/threonine kinase 38 (STK38)
TC115604 NM_007284 Homo sapiens PTK9L protein tyrosine kinase 9-like (A6-related protein) (PTK9L)
TC115594 NM_007314 Homo sapiens v-abl Abelson murine leukemia viral oncogene homolog 2 (arg, Abelson-related gene) (ABL2), transcript variant b
TC115567 NM_007371 Homo sapiens bromodomain containing 3 (BRD3)
TC111954 NM_012119 Homo sapiens cell cycle related kinase (CCRK), OriGene unique variant 1 TC124172 NM_012119 Homo sapiens cell cycle related kinase (CCRK), transcript variant 2 TC108453 NM_012290 Homo sapiens tousled-like kinase 1 (TLK1)
TC115413 NM_012395 Homo sapiens PFTAIRE protein kinase 1 (PFTK1), OriGene unique variant 1 TC115380 NM_012424 Homo sapiens ribosomal protein S6 kinase, 52kDa, polypeptide 1 (RPS6KC1) TC115335 NM_013233 Homo sapiens serine threonine kinase 39 (STE20/SPS1 homolog, yeast) (STK39) TC111251 NM_013254 Homo sapiens TANK-binding kinase 1 (TBK1)
TC115312 NM_013257 Homo sapiens serum/glucocorticoid regulated kinase-like (SGKL), transcript variant 1 TC108619 NM_013355 Homo sapiens protein kinase N3 (PKN3)
TC115246 NM_013392 Homo sapiens nuclear receptor binding protein (NRBP)
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