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Efficient Expression of Genes in the Drosophila Germline Using a UAS Promoter Free of Interference by Hsp70 piRNAs

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Academic year: 2020

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Figure

Figure 1 Components of commonin place of the SV40 terminator and Hsp70 sequences. We created two new UASp vectors, pUASpGFPattB and pMRpGFP, based on pJFRC81andpMRtGFP, to directly compare the effect of P-element and Hsp70 sequences on transgene expression
Figure 2 Expression from UASt is greater than UASp in cells lacking Hsp70 piRNAs. (A–D) pMRtGFP (UAStGFP) and pMRpGFP (UASpGFP) integratedinto the same MiMIC site (Mi04106) and crossed to either a control without Gal4 to visualize UAS-GFP leakiness, Tub-Ga
Figure 3 Expression level ofsamples. (F) Average GFP pixel intensity in germ cells of the indicated stage and genotype

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