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Therapeutic efficiency of folated poly(ethylene glycol)-chitosan-graft-polyethylenimine-<em>Pdcd4</em> complexes in H-<em>ras</em>12V mice with liver cancer

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Figure 1 (A) Proposed reaction scheme for synthesis of FPCP. (B) Representative 1H nuclear magnetic resonance spectra for FPCP in D2O: δ = 6.8 ppm (=CH−, folic acid), δ = 3.6–3.1 ppm (−CH2CH2O−, PEG ethylene), δ = 2.8–2.2 ppm (−NHCH2CH2−, PEI ethylene), and δ = 2.0–1.9 ppm (−CH3, chitosan methylene).Abbreviations: NHS, N-hydroxysuccinimide; DCC, dicyclohexylcarbodiimide; EDC, N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydro chloride; PEI, polyethyl-enimine; FPCP, folated poly(ethylene glycol)-chitosan-graft-polyethylenimine; Fol, folate; PEG, polyethylene glycol.
Figure 2 Characterizations of FPCP-DNA complexes. (A) Agarose gel electrophoresis of FPCP-DNA complexes at various N/P ratios
Figure 3 Cytotoxicity of the FPCP copolymer at various concentrations in different cell lines
Figure 4 (A) Transfection efficiency of FPCP-DNA complexes in KB/F cells. (B) Effect of bafilomycin A1 on gene transfection
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