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Major contribution of the RNA binding domain of NS1 in the pathogenicity and replication potential of an avian H7N1 influenza virus in chickens

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Academic year: 2020

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Figure

Fig. 1 Virus pathogenicity. Four-week old SPF chickens were inoculated with the three wild-type (wt) and mutant viruses
Fig. 2 Growth properties of the three viruses. (virus titres (EIDLeft panel) The three indicated viruses were inoculated in embryonated chicken eggs to determine50) and the HA titres in the allantoic fluids of individual eggs were recorded
Fig. 3 Accumulation of NS1 in virus-infected CLEC213 cells. Subconfluent CLEC213 cells in a 24-well plate were infected (0.5 PFU/cell) and lysedat the indicated times post-infection
Fig. 4 Subcellular distribution of NS1 in virus-infected CLEC213 cells. CLEC213 cells were mock-infected or infected with the indicated viruses(0.5 PFU/cell), then fixed and immunostained with an NS1-specific rabbit antiserum at 12 h or 24 h p.i
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