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Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off target effects

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Figure

Fig. 1 F-A locus and target site selection.transfected with Cas9 only (without sgRNA)
Fig. 2 Process and characteristics of dCas9 binding in BFFs.with the guiding sequences of sgRNA45 (binding site.were localized around four on-target sites and the control
Fig. 3 Off-target sites of sgRNA 45 and 20 with the top 15 ChIP-seq binding densities
Fig. 4 Single Cas9n stimulated HDR at the F-A locus.framesrecombined with the genome through single Cas9-induced DSB ( a Experiential outline and schematic of the HDR process
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