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Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells

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Figure

FIG 1 Isolation and characterization of TESA exovesicles. (A) Crude TESA isolated from infected Vero cellculture supernatant was concentrated by ultrafiltration, and a supernatant (lane 1) and EV pellets fromtwo different batches (lanes 2 and 3) were isolat
FIG 2 Biological function of protein in TESA EVs. Purified TESA EVs from at least two biological replicatedwere analyzed by nano-LC–MS/MS, and proteins were identified by searching the T
FIG 3 Immunoreactivity of TESA with Chagasic sera. (A) Western blots containing whole-cell extracts oftigotes (lane 1),sera from Colombian uninfected controls or Chagasic patients with various stages of cardiopathologies as previously detailed(54)
TABLE 1 Most abundant immunoaffinity-purified proteins from TESA extracellular vesiclesa
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