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O -GlcNAcylation of ATG4B positively regulates autophagy by increasing its hydroxylase activity

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Figure

Figure 1: Treatment with PugNAc, an OGA inhibitor activates autophagy. fluorescence microscopy (B)
Figure 2: OGT directly binds to ATG4B in SH-SY5Y cells. A. SH-SY5Y cells were transiently co-transfected with BiFC plasmids [(pFlag/OGT–VN173 (OGT-VN) and pMYC/ATG–VC155 (ATGx-VC)]
Figure 4D, change of culture media form low to high
Figure 4: Low glucose condition promotes O-GlcNAcylation of ATG4B. A. SH-SY5Y/HA-ATG4B cells were cultured in normal (4.5 mg/ml glucose) or low glucose condition (Low glu, 1 mg/ml glucose)
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