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Rad53 homologue forkhead-associated kinase A (FhkA) and Ca2+-binding protein 4a (CBP4a) are nucleolar proteins that differentially redistribute during mitosis in Dictyostelium

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Academic year: 2020

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Figure

Figure 1 Domain structure diagram of FhkA and CBP4a. (A) FhkA (Rad53 in yeast, Chk2 in humans) possesses several putative FHA domains(asterisk indicates verified domains [47,48])
Figure 2 Localization of FhkA and CBP4a. (A) Unlike for cellsprobed with preimmune serum, which displayed diffuse/weakfluorescence, anti-FhkA detected FhkA in untreated cells at the edge ofnucleolar patches as demonstrated by colocalization with DAPIcounte
Figure 3 Effect of AM-D on FhkA and CBP4a localization. (A)Four hour treatment also resulted in detection of CBP4a in the centrosomal region (Ct) while 8 hour treatment resulted in the presence ofcytoplasmic particles (CP)
Figure 5 FhkA redistributes throughout the cytoplasm duringmitosis. The pattern of microtubules (α-tubulin) was used todetermine the stage of mitosis and DAPI was used to identify thenucleus
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